Polymeric nanofibrous scaffolds laden with cell-derived extracellular matrix for bone regeneration

Junka, Radoslaw; Yu, Xiaojun

doi:10.1016/j.msec.2020.110981

Cited by 25 publications

(15 citation statements)

References 67 publications

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“…Osteocalcin is involved in the recruitment, differentiation and maturation of osteoclasts [ 32 ]. Scaffolds prepared from the decellularized extracellular matrix of porcine cancellous bone increased the expression of osteocalcin and supported MSC differentiation [ 33 ]. BMP2 is an effective osteoinductive cytokine that plays a vital role during bone regeneration and repair.…”

Section: Discussionmentioning

confidence: 99%

Supercritical Carbon Dioxide Decellularized Bone Matrix Seeded with Adipose-Derived Mesenchymal Stem Cells Accelerated Bone Regeneration

et al. 2021

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Large bone fractures with segmental defects are a vital phase to accelerate bone integration. The present study examined the role of supercritical carbon dioxide (scCO2) decellularized bone matrix (scDBM) seeded with allogeneic adipose-derived mesenchymal stem cells (ADSC) as bio-scaffold for bone regeneration. Bio-scaffold produced by seeding ADSC to scDBM was evaluated by scanning electron microscopy (SEM). Rat segmental femoral defect model was used as a non-union model to investigate the callus formation in vivo. Histological analysis and osteotomy gap closure in the defect area were analyzed at 12 and 24 weeks post-surgery. Immunohistochemical expression of Ki-67, BMP-2 and osteocalcin was evaluated to assess the ability of new bone formation scDBM. ADSC was found to attach firmly to scDBM bioscaffold as evidenced from SEM images in a dose-dependent manner. Callus formation was observed using X-ray bone imaging in the group with scDBM seeded with 2 × 106 and 5 × 106 ASCs group at the same time-periods. H&E staining revealed ASCs accelerated bone formation. IHC staining depicted the expression of Ki-67, BMP-2, and osteocalcin was elevated in scDBM seeded with 5 × 106 ASCs group at 12 weeks after surgery, relative to other experimental groups. To conclude, scDBM is an excellent scaffold that enhanced the attachment and recruitment of mesenchymal stem cells. scDBM seeded with ASCs accelerated new bone formation.

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Section: Discussionmentioning

confidence: 99%

Supercritical Carbon Dioxide Decellularized Bone Matrix Seeded with Adipose-Derived Mesenchymal Stem Cells Accelerated Bone Regeneration

et al. 2021

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“…The opportunity to fabricate complex composite constructions mimicking bone structure and promoting endogenous cells to recover bone tissue laid the foundation to the ubiquitous study of synthetic polymer matrices enriched with dECM biochemical cues. The study [ 67 ] discusses the effect of cell-derived dECM incorporation into PCL-based solutions on the biochemical properties of bi-layered nanofibrous scaffolds with osteogenic and vascular cues obtained via electrospinning. According to bioactivity investigation in osteoblast cultures, dECM-containing scaffolds exhibited sufficient cell proliferation, Alizarin Red staining, and osteopontin-positive extracellular deposits.…”

Section: Modern Polymer-based Fibrous Compositesmentioning

confidence: 99%

Fibrous Polymer-Based Composites Obtained by Electrospinning for Bone Tissue Engineering

2021

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Currently, the significantly developing fields of tissue engineering related to the fabrication of polymer-based materials that possess microenvironments suitable to provide cell attachment and promote cell differentiation and proliferation involve various materials and approaches. Biomimicking approach in tissue engineering is aimed at the development of a highly biocompatible and bioactive material that would most accurately imitate the structural features of the native extracellular matrix consisting of specially arranged fibrous constructions. For this reason, the present research is devoted to the discussion of promising fibrous materials for bone tissue regeneration obtained by electrospinning techniques. In this brief review, we focus on the recently presented natural and synthetic polymers, as well as their combinations with each other and with bioactive inorganic incorporations in order to form composite electrospun scaffolds. The application of several electrospinning techniques in relation to a number of polymers is touched upon. Additionally, the efficiency of nanofibrous composite materials intended for use in bone tissue engineering is discussed based on biological activity and physiochemical characteristics.

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“…Furthermore, they found that the bone-dECM particle-integrin interaction induced by the cell adhesive proteins regulated the intracellular processes and enhanced the cell adhesion/migration. Junka et al [61] used sequential electrospinning with PCL solution containing either osteoblast or endothelial cells-derived dECM to fabricate two-layer scaffolds. The nanofibrous scaffolds incorporated with angiogenic and osteogenic cues could stimulate bone growth.…”

Section: Ecm Simulationmentioning

confidence: 99%

Fibers by Electrospinning and Their Emerging Applications in Bone Tissue Engineering

et al. 2021

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Bone tissue engineering (BTE) is an optimized approach for bone regeneration to overcome the disadvantages of lacking donors. Biocompatibility, biodegradability, simulation of extracellular matrix (ECM), and excellent mechanical properties are essential characteristics of BTE scaffold, sometimes including drug loading capacity. Electrospinning is a simple technique to prepare fibrous scaffolds because of its efficiency, adaptability, and flexible preparation of electrospinning solution. Recent studies about electrospinning in BTE are summarized in this review. First, we summarized various types of polymers used in electrospinning and methods of electrospinning in recent work. Then, we divided them into three parts according to their main role in BTE, (1) ECM simulation, (2) mechanical support, and (3) drug delivery system.

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Polymeric nanofibrous scaffolds laden with cell-derived extracellular matrix for bone regeneration

Cited by 25 publications

References 67 publications

Supercritical Carbon Dioxide Decellularized Bone Matrix Seeded with Adipose-Derived Mesenchymal Stem Cells Accelerated Bone Regeneration

Supercritical Carbon Dioxide Decellularized Bone Matrix Seeded with Adipose-Derived Mesenchymal Stem Cells Accelerated Bone Regeneration

Fibrous Polymer-Based Composites Obtained by Electrospinning for Bone Tissue Engineering

Fibers by Electrospinning and Their Emerging Applications in Bone Tissue Engineering

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