Polymeric nanocapsules and nanospheres for encapsulation and long sustained release of hydrophobic cyanine-type photosensitizer

Bazylińska, Urszula; Lewińska, Agnieszka; Lamch, Łukasz; Wilk, Kazimiera A.

doi:10.1016/j.colsurfa.2013.02.023

Cited by 76 publications

(61 citation statements)

References 28 publications

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“…Furthermore, IR-780 encapsulated by nanoemulsions covered by four PE bilayers (nanocapsules 2) photobleaches slightly lower than when enclosed in nanocapsules with thinner coat (nanocapsules 1) (see Table I). The observed phenomenon confirmed the earlier findings, 33,39 indicating that both the nanoparticles' oil core and PE shell thickness may significantly enhance the photostability of the hydrophobic photosensitizer. Other important in vitro features of the encapsulated IR-780 such as reactive oxygen species (ROS) and release characteristics are supplemented in the supplementary material (Figs.…”

Section: -8supporting

confidence: 80%

“…39 All the irradiation experiments were performed using an OPTEL Fibre Illuminator (Opole, Poland) equipped with a band pass glass filter (SCHOTT GLASWERKE GmbH) to isolate the 760-800 nm spectral interval. The rate of photobleaching was evaluated by measuring the cyanine absorption spectrum in the 500-800 nm range upon exposure of 3 ml solutions containing 4 lM IR-780 or its encapsulated form to the lamp operating at the energy dose of 100 mW/cm 2 .…”

Section: Encapsulation and Photobleaching Of Ir-780 Cyaninementioning

confidence: 99%

“…¼ 805 nm) wavelengths and high molecular extinction coefficient (e ¼ 27.4 Â 10 4 M À1 cm À1 ) has been adopted so far as a non-targeting contrast agent in clinical and experimental NIR imaging and as a promising second generation photosensitizer in PDT. [36][37][38][39] However, IR-780 as many other hydrophobic cyanines can be disposed to light-induced decomposition (i.e., photobleaching) both in vitro and in vivo resulting in the loss of absorbance, fluorescence, and photoactivity, and thus possibly limiting its use in PDT. 40 In order to increase the potential of hydrophobic cyanines towards improvement of drug efficacy, its stability, and better intracellular penetration, they need to be encapsulated in nanocarriers to avoid any unexpected problems with photoactivity and to improve their dissolution performance by means of encapsulation via template-mediated processes.…”

Section: Introductionmentioning

confidence: 99%

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Microfluidic platform for photodynamic therapy cytotoxicity analysis of nanoencapsulated indocyanine-type photosensitizers

et al. 2016

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The application of nanotechnology is important to improve research and development of alternative anticancer therapies. In order to accelerate research related to cancer diagnosis and to improve the effectiveness of cancer treatment, various nanomaterials are being tested. The main objective of this work was basic research focused on examination of the mechanism and effectiveness of the introduction of nanoencapsulated photosensitizers to human carcinoma (A549) and normal cells . Newly encapsulated hydrophobic indocyanine-type photosensitizer (i.e., IR-780) was subjected to in vitro studies to determine its release characteristics on a molecular level. The photosensitizers were delivered to carcinoma and normal cells cultured under model conditions using multiwell plates and with the use of the specially designed hybrid (poly(dimethylsiloxane) (PDMS)/ glass) microfluidic system. The specific geometry of our microsystem allows for the examination of intercellular interactions between cells cultured in the microchambers connected with microchannels of precisely defined length. Our microsystem allows investigating various therapeutic procedures (e.g., photodynamic therapy) on monoculture, coculture, and mixed culture, simultaneously, which is very difficult to perform using standard multiwell plates. In addition, we tested the cellular internalization of nanoparticles (differing in size, surface properties) in carcinoma and normal lung cells. We proved that cellular uptake of nanocapsules loaded with cyanine IR-780 in carcinoma cells was more significant than in normal cells. We demonstrated non cytotoxic effect of newly synthesized nanocapsules built with polyelectrolytes (PEs) of opposite surface charges: polyanion-polysodium-4-styrenesulphonate and polycation-poly(diallyldimethyl-ammonium) chloride loaded with cyanine IR-780 on human lung carcinoma and normal cell lines. However, the differences observed in the photocytotoxic effect between two types of tested nanocapsules can result from the type of last PE layer and their different surface charge. V C 2016 AIP Publishing LLC. [http://dx

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Section: -8supporting

confidence: 80%

Section: Encapsulation and Photobleaching Of Ir-780 Cyaninementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Microfluidic platform for photodynamic therapy cytotoxicity analysis of nanoencapsulated indocyanine-type photosensitizers

et al. 2016

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“…Authors confirm that the higher the hydrophobicity of the compound, the slower the release process. The release of hydrophobic IR-780 iodide dyes encapsulated in NS and NC prepared with biodegradable polymer no water soluble poly(D,L-lactide) (PLA) or polycaprolactone (PCL) was evaluated by Bazylińska et al [20]. They observed slower release rates in NC and confirmed that hydrophobic dye can be more effective when enclosed in the oleic core of a NC than when enclosed only in the polymeric matrix of a NS.…”

Section: International Journal Of Polymer Sciencementioning

confidence: 67%

Method Validation for Progesterone Determination in Poly(methyl methacrylate) Nanoparticles Synthesized via Miniemulsion Polymerization

Fogolari

Felippe

Leimann

et al. 2017

International Journal of Polymer Science

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Exogenous progesterone has several applications in human health and in veterinary medicine, especially in fixed-time artificial insemination protocol. Progesterone nanoencapsulation in biocompatible polymers, such as poly(methyl methacrylate) (PMMA), is an alternative to substitute silicone-based release device traditionally used for estrus control. Progesterone concentration inside the nanoparticles must be precisely known; for that reason, a validation methodology must be applied to ensure reliable results, suitable for nanoparticles application. In this work, an UV-Vis spectrophotometric method was validated for the determination of progesterone in PMMA nanoparticles synthesized by miniemulsion polymerization. Chloroform was used as solvent, showing selectivity to the encapsulated drug and the components of the polymeric matrix did not influence progesterone recovery. Detection and quantitation limits (DL and QL) obtained were 0.32 and 0.96 mg⋅L −1 , respectively, and precision tests (between different analysts and equipment) indicated acceptable Relative Standard Deviations (RSD < 5%). Miniemulsion polymerization reactions were carried out producing two different morphologies: nanospheres (NS) and nanocapsules (NC), with average intensity diameters (Dz) of 150-200 nm and 240-300 nm, respectively. Polymerization gravimetric conversions obtained for both cases were higher than 95% and encapsulation efficiencies greater than 69% and 90% for the nanospheres and nanocapsules, respectively.

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“…Polymeric nanocapsules were fabricated by a direct solvent displacement (also called nanoprecipitation) according to the procedure reported by our group [28]. Briefly, an organic solution consisting of PLA (5 mg/ml), palm oil (0.1 mg/mL) and Cou-6 (0.1 mg/mL) or/and Col (0.2 mg/mL) dissolved in THF was prepared.…”

Section: Preparation Of Nanoparticles -Empty and Loaded With Theranosmentioning

confidence: 99%

New diamidequat-type surfactants in fabrication of long-sustained theranostic nanocapsules: Colloidal stability, drug delivery and bioimaging

Bazylińska

Zieliński

Kulbacka

et al. 2016

Colloids and Surfaces B: Biointerfaces

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Polymeric nanocapsules and nanospheres for encapsulation and long sustained release of hydrophobic cyanine-type photosensitizer

Cited by 76 publications

References 28 publications

Microfluidic platform for photodynamic therapy cytotoxicity analysis of nanoencapsulated indocyanine-type photosensitizers

Microfluidic platform for photodynamic therapy cytotoxicity analysis of nanoencapsulated indocyanine-type photosensitizers

Method Validation for Progesterone Determination in Poly(methyl methacrylate) Nanoparticles Synthesized via Miniemulsion Polymerization

New diamidequat-type surfactants in fabrication of long-sustained theranostic nanocapsules: Colloidal stability, drug delivery and bioimaging

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