Method Validation for Progesterone Determination in Poly(methyl methacrylate) Nanoparticles Synthesized via Miniemulsion Polymerization

Abstract

Exogenous progesterone has several applications in human health and in veterinary medicine, especially in fixed-time artificial insemination protocol. Progesterone nanoencapsulation in biocompatible polymers, such as poly(methyl methacrylate) (PMMA), is an alternative to substitute silicone-based release device traditionally used for estrus control. Progesterone concentration inside the nanoparticles must be precisely known; for that reason, a validation methodology must be applied to ensure reliable results, suitable for nanoparticles application. In this work, an UV-Vis spectrophotometric method was validated for the determination of progesterone in PMMA nanoparticles synthesized by miniemulsion polymerization. Chloroform was used as solvent, showing selectivity to the encapsulated drug and the components of the polymeric matrix did not influence progesterone recovery. Detection and quantitation limits (DL and QL) obtained were 0.32 and 0.96 mg⋅L −1 , respectively, and precision tests (between different analysts and equipment) indicated acceptable Relative Standard Deviations (RSD < 5%). Miniemulsion polymerization reactions were carried out producing two different morphologies: nanospheres (NS) and nanocapsules (NC), with average intensity diameters (Dz) of 150-200 nm and 240-300 nm, respectively. Polymerization gravimetric conversions obtained for both cases were higher than 95% and encapsulation efficiencies greater than 69% and 90% for the nanospheres and nanocapsules, respectively.

Single step encapsulation process of ivermectin in biocompatible polymer using a supercritical antisolvent system process

“…For the SEM analysis, the samples were evenly distributed on metalized tape and inserted into the equipment at a potential of 15 kV under low vacuum 20 . For the latter, the samples were diluted (0.05% v/v), placed on copper grids (Formvar/Carbon Support Film, 100 mesh, 3.05 mm in diameter, TAAB), and coated with cathodic fluid spray to prevent sample degradation due to the electron beam 24 . TGA and DTG analyses were performed in a Netzsch STA 409PC (Netzsch Corporation, Germany).…”

State-of-the-Art and Prospective of Nanotechnologies for Smart Reproductive Management of Farm Animals

“…The main matrix of these devices is composed of silicon polymers, which need to be loaded with high progesterone concentrations to release enough hormones to the vaginal mucosa. Progesterone levels into the device remain high after discard and currently, inserts based on polyethylene vinyl acetate (EVA) copolymers polyisoprene and polymethyl-methacrylate are being developed for reducing progesterone charge, thus diminishing the costs and emissions of hormones to the environment [2,42]. However, similarly to silicone-based devices, these materials are not biodegradable.…”

“…Oliveira and coworkers [15] developed and tested biodegradable and biocompatible nanomaterials (specifically nanofibrous mats of polylactic acid; PLA) loaded with progesterone by solution blow spinning technique, with promising results for controlled progesterone delivery. Similarly, Fogolari and coworkers [42] used a method of miniemulsion polymerization for producing two forms of progesterone-conjugated nanocapsules: nanospheres (NS) and nanocapsules (NC), using polymethyl-methacrylate, a biocompatible polymer, instead of silicone-based release devices. The encapsulation efficiencies of NS and NC were greater than 69% and 90%, with average sizes of 150-200 nm and 240-300 nm, respectively, which supports the usefulness of this method for progesterone binding.…”

Dissolution Improvement of Progesterone and Testosterone via Impregnation on Mesoporous Silica Using Supercritical Carbon Dioxide

“…Both TST and PRG are classified as class II according to the Biopharmaceutics Classification System (BCS) and class IIb as per the Developability Classification System (DCS) [70]. Until now, there have been several studies to improve the dissolution of PRG and TST, including the formation of cocrystals [71], cyclodextrin complexation [72][73][74][75][76], polymeric nanoparticles [77,78], micro-/mesoporous materials [79][80][81], and micronisation via rapid expansion of supercritical solution [82]. The supercritical impregnation process is a simple technique with several advantages over conventional methods, including (i) homogenous distribution of active substance within the solid matrix, (ii) environmentally friendly technique for the reduction of waste and the use of toxic organic solvents, (iii) shorter processing times as there is no requirement for an additional drying step, and (iv) any excess drug active can be recycled by avoiding cross-contamination [83].…”