Polylysine-bilirubin conjugates maintain functional islets and promote M2 macrophage polarization

Zhao, Ying‐Zheng; Huang, Zhiwei; Zhai, Yuanyuan; Shi, Yannan; Du, Chu-Chu; Zhai, Jiaoyuan; Xu, He‐Lin; Xiao, Jian; Kou, Longfa; Yao, Qing

doi:10.1016/j.actbio.2020.12.047

Cited by 27 publications

(21 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Characterization and Cytotoxicity of CS-BR CS-BR was synthesized via the EDC/NHS-based carboxyl-ammonia condensation reaction (Figure 1A), as previously reported. 17 The obtained CS-BR was confirmed by 1 H-NMR analysis. The 1 H-NMR spectra of CS exhibited a peak at 5.1 ppm due to anomeric proton (H1), the signals between 3.2 ppm and 3.7 ppm corresponding to protons H3-H6 of the polysaccharide ring, and the signal at 2.9 ppm is due to the H2 proton of the amine group (H-amine).…”

Section: Resultsmentioning

confidence: 70%

“…Thus, the therapeutic concentration of BR is relatively narrow, and researchers must pay attention to the adverse effects of BR during disease treatments. We have screened for the optimal BR concentration (20 μM) in islet cells 17 and then used a relatively conservative dose (5 mg/kg) to treat acute kidney injury. 19 In this study, we also selected a low concentration of bilirubin for GU to allow BR to exert curative effects without causing significant toxic effects to the body.…”

Section: Resultsmentioning

confidence: 99%

“… 16 In addition, previous studies in our laboratory showed that BR could modulate macrophages toward the M2-type and enhance the therapeutic effect of islet transplantation in diabetes mellitus. 17 However, BR is physiologically insoluble in water, which greatly limits its application in research. Thus, researchers have developed various pharmaceutical preparations for BR that have enhanced water solubility and optimized pharmacokinetics.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Protective Effects of Chitosan-Bilirubin Nanoparticles Against Ethanol-Induced Gastric Ulcers

Huang¹,

Shi²,

Wang³

et al. 2021

IJN

Self Cite

View full text Add to dashboard Cite

Gastric ulcers (GU) are a disease of the gastrointestinal tract that can be caused by excessive alcohol consumption and heavy use of nonsteroidal anti-inflammatory drugs. GU manifests predominantly as pathological damage, such as extensive inflammatory erosion and superficial bleeding of the gastric mucosa. Oxidative stress damage and the inflammatory response are now considered important predisposing factors for GU, suggesting that antioxidant and anti-inflammatory drugs could be treatments for GU. Nanoparticle drug carriers offer many advantages over conventional drugs, such as improved drug efficiency, increased drug stability, and increased half-life. Methods: We designed chitosan-bilirubin conjugate (CS-BR) nanoparticles and assessed the anti-inflammatory and antioxidant abilities of CS-BR in gastric epithelial cells. Then, we evaluated the intragastric retention time and the anti-ulcer effects of CS-BR in vivo. Results:The in vitro data showed that CS-BR nanoparticles protect gastric epithelial cells against oxidative/inflammatory injury. The in vivo study demonstrated that CS-BR nanoparticles accumulate permanently in the stomach and exert powerful antioxidant and antiinflammatory effects against GU. Conclusion:This study applied bilirubin to the treatment of GU and confirmed that CS-BR nanoparticles are effective at alleviating acute GU in an experimental model. The findings provide innovative ideas for prophylaxis against or treatment of GU.

show abstract

Section: Resultsmentioning

confidence: 70%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Protective Effects of Chitosan-Bilirubin Nanoparticles Against Ethanol-Induced Gastric Ulcers

Huang¹,

Shi²,

Wang³

et al. 2021

IJN

Self Cite

View full text Add to dashboard Cite

show abstract

“…These results were associated with significant reduction in inflammatory cytokines, including interleukin 1 β (IL-1β), tumor necrosis factor-α (TNF-α), MCP-1, and NO, suggesting additional anti-inflammatory properties of bilirubin. Furthermore, bilirubin also increased vascularization, as evidenced by increased blood vessel formation in ε-polylysine-bilirubin conjugate (PLL-BR) encapsulated islet cells transplanted into diabetic mice compared to control [ 90 ]. Unfortunately, its clinical applications have been limited due to its insolubility and short half-life.…”

Section: Antioxidative Treatments During Islet Cell Infusionmentioning

confidence: 99%

Anti-Oxidative Therapy in Islet Cell Transplantation

et al. 2022

View full text Add to dashboard Cite

Islet cell transplantation has become a favorable therapeutic approach in the treatment of Type 1 Diabetes due to the lower surgical risks and potential complications compared to conventional pancreas transplantation. Despite significant improvements in islet cell transplantation outcomes, several limitations hamper long-term graft survival due to tremendous damage and loss of islet cells during the islet cell transplantation process. Oxidative stress has been identified as an omnipresent stressor that negatively affects both the viability and function of isolated islets. Furthermore, it has been established that at baseline, pancreatic β cells exhibit reduced antioxidative capacity, rendering them even more susceptible to oxidative stress during metabolic stress. Thus, identifying antioxidants capable of conferring protection against oxidative stressors present throughout the islet transplantation process is a valuable approach to improving the overall outcomes of islet cell transplantation. In this review we discuss the potential application of antioxidative therapy during each step of islet cell transplantation.

show abstract

“…In addition, Zhao et al. reported a ε-polylysine-Bi conjugate (PLL-BR) to embedding the islets to study its function on macrophage modulation activity during islet transplantation [ 103 ]. The PLL-BR was able to target and cluster to islets and exhibit excellent antioxidants and anti-inflammatory properties, and it was the first time to demonstrate that Bi and its derivatives could efficaciously accelerate the polarization of M2 macrophage and ameliorate the immune microenvironment to maintain functional islets.…”

Section: Ros-scavenging Nanomaterials That Promote Oxidative Damage Repair and Mechanisms Governing This Repairmentioning

confidence: 99%

Reactive-oxygen-species-scavenging nanomaterials for resolving inflammation

Huang

Zheng

et al. 2021

Materials Today Bio

View full text Add to dashboard Cite

Reactive oxygen species (ROS) mediate multiple physiological functions; however, the over-accumulation of ROS causes premature aging and/or death and is associated with various inflammatory conditions. Nevertheless, there are limited clinical treatment options that are currently available. The good news is that owing to the considerable advances in nanoscience, multiple types of nanomaterials with unique ROS-scavenging abilities that influence the temporospatial dynamic behaviors of ROS in biological systems have been developed. This has led to the emergence of next-generation nanomaterial-controlled strategies aimed at ameliorating ROS-related inflammatory conditions. Accordingly, herein we reviewed recent progress in research on nanotherapy based on ROS scavenging. The underlying mechanisms of the employed nanomaterials are emphasized. Furthermore, important issues in developing cross-disciplinary nanomedicine-based strategies for ROS-based inflammatory conditions are discussed. Our review of this increasing interdisciplinary field will benefit ongoing studies and clinical applications of nanomedicine based on ROS scavenging.

show abstract

Polylysine-bilirubin conjugates maintain functional islets and promote M2 macrophage polarization

Cited by 27 publications

References 43 publications

Protective Effects of Chitosan-Bilirubin Nanoparticles Against Ethanol-Induced Gastric Ulcers

Protective Effects of Chitosan-Bilirubin Nanoparticles Against Ethanol-Induced Gastric Ulcers

Anti-Oxidative Therapy in Islet Cell Transplantation

Reactive-oxygen-species-scavenging nanomaterials for resolving inflammation

Contact Info

Product

Resources

About