Polyhydroxybutyrate production using agro-industrial residue as substrate by Bacillus sphaericus NCIM 5149

Abstract

The aim of this work was to study the production of polyhydroxybutyrate (PHB) using agro-industrial residues as the carbon source. Seven substrates, viz., wheat bran, potato starch, sesame oil cake, groundnut oil cake, cassava powder, jackfruit seed powder and corn flour were hydrolyzed using commercial enzymes and the hydrolyzates assessed for selecting the best substrate for PHB production. Jackfruit seed powder gave the maximum production of PHB under submerged fermentation using Bacillus sphaericus (19%) at the initial pH of 7.5.

Agrowaste-based Polyhydroxyalkanoate (PHA) production using hydrolytic potential of Bacillus thuringiensis IAM 12077

“…This could be attributed to these substrates being rich in protein, or other nutrients as well that triggered the growth phase instead of nutrient depletion. Similar observations were made by Van-Thuoc et al (2007) and Ramadas et al (2009) wherein wheat bran and cassava bagasse supported good growth but low PHB accumulation. There was a positive correlation between the amount of reducing sugar released [data not shown] by acid hydrolysis of the substrates with the PHB yield obtained (r = 0.70538).…”

“…Rusendi and John (1995) used waste potato starch hydrolyzate for the production of PHB and reported a yield of 77% of the biomass dry weight. Ramadas et al (2009) …”

Production of biopolyester poly(3-hydroxybutyrate) by Bacillus cereus RCL 02, a leaf endophyte of Ricinus communis L.

“…Development of newer fermentation strategies along with use of nonconventional renewable carbon sources including different plant oils [17,18], molasses [19], oil cake extracts [20], crude glycerol [21] and agricultural wastes [22] have been explored to reduce the cost of PHAs production and making the process economically viable.…”

Modelling antagonic effect of lactic acid eacteria supernatants on some pathogenic bacteria

“…Different inoculum size varying from 1 to 5 ml was tested for the effect of inoculum size on supernatants production (Ramadas, et al, 2009) and three different species of Lactobacillus were obtained from the American Type Culture Collection (ATCC) and from the Department of Bioprocess Engineering and Biotechnology (DEBB), Federal University of Paraná. These strains were tested and used as antimicrobial producing starters.…”