Agrowaste-based Polyhydroxyalkanoate (PHA) production using hydrolytic potential of Bacillus thuringiensis IAM 12077

Gowda, Vaishnavi; Shivakumar, Srividya

doi:10.1590/s1516-89132014000100009

Cited by 103 publications

(45 citation statements)

References 16 publications

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“…Similarity, B. subtilis and B. thuringiensis were able to produce about 60-65 wt% of P(3HB) from industrial food waste and starch (Gowda and Shivakumar, 2014). Glycerol is a preferred carbon source for PHA production by B. megaterium (Gómez Cardozo et al, 2016).…”

Section: Biosynthesis Of Pha Using Different Agro-industry By-productsmentioning

confidence: 90%

Bioconversion of novel and renewable agro-industry by-products into a biodegradable poly(3-hydroxybutyrate) by marine Bacillus megaterium UMTKB-1 strain

Yatim¹,

Syafiq²,

Huong³

et al. 2017

bta

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Agro-industry by-products are abundant in various valuable compounds. Some of these raw materials are considered as a cheaper carbon source for polyhydroxyalkanoate (PHA) production when compared with pure substrates. It is however often a costly affair for industries to recover the residual carbon components. In this study, poly(3-hydroxybutyrate) [P(3HB)] was produced using a marine Bacillus megaterium UMTKB-1 strain from sweetwater, a by-product from cane sugar refining process. The bioconversion was initiated in shaken-flasks and fermenter experiments. Applications of seawater and sweetwater mixture as well as sweetwater only as PHA culture media were investigated. The produced polymer was characterized using Gas Chromatography (GC) and Gel Permeation Chromatography (GPC). P(3HB) accumulation from tested carbon sources ranged from 3 to 49 wt%. The strain could accumulate P(3HB) in saline conditions when minimal salts medium was replaced with seawater and sweetwater. The P(3HB) content was between 7 and 27 wt%. This strain was also able to grow and accumulate up to 14 wt% P(3HB) when sweetwater was the sole PHA biosynthesis medium. The weights of P(3HB) produced was in the range 3-12 × 10 5 with polydispersity index values ranging from 2.7 to 3.8. The agroindustry by-products have proven to be potential carbon feedstocks for P(3HB) production. The tested strain was able to grow and accumulate P(3HB) in a novel carbon substrate medium, the sweetwater. This by-product could be used as a raw material for P(3HB) production without any pretreatment.

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Section: Biosynthesis Of Pha Using Different Agro-industry By-productsmentioning

confidence: 90%

Bioconversion of novel and renewable agro-industry by-products into a biodegradable poly(3-hydroxybutyrate) by marine Bacillus megaterium UMTKB-1 strain

Yatim¹,

Syafiq²,

Huong³

et al. 2017

bta

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“…[342,[351][352][353] Rice straw hydrolysate can also be used by using Bacillusf irmus for P(3HB)p roduction, [355] whereas rice bran hydrolysate can be fermented by S. meliloti into P(3HB-co-3HV) copolyesters. Bagasse (i.e.,t he fibrousm atter of sugarcane that remains after molasses extraction) hydrolysate, containing mainly xylose, can be used to obtain scl-PHAs, mostly P(3HB), by using B. cepacia, Burkholderia sacchari, C. necator, Saccharophagus degradans,o rB.…”

Section: Carbohydratesmentioning

confidence: 99%

“…Some significant starchy waste streamsf rom food production can be used by PHA-producing microorganisms. Starch hydrolysates can be used to produce PHAs (mostly P(3HB)) by using Massilia strains, [341] Bacillust huringiensis, [342] B. cereus, [343] Alcaligenes eutrophus, [344] Haloferax mediterranei, [345] Cupriavidus strains, [321,346] Azotobacter chroococcum, [347] and Bacillus tequilensis. [348] P(3HBco-3HV) copolyesters with about 10 mol %4 HV can be obtained from H. mediterranei by using corn starch as ac arbon source.…”

Section: Carbohydratesmentioning

confidence: 99%

Biotic and Abiotic Synthesis of Renewable Aliphatic Polyesters from Short Building Blocks Obtained from Biotechnology

2018

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Biobased polymers have seen their attractiveness increase in recent decades thanks to the significant development of biorefineries to allow access to a wide variety of biobased building blocks. Polyesters are one of the best examples of the development of biobased polymers because most of them now have their monomers produced from renewable resources and are biodegradable. Currently, these polyesters are mainly produced by using traditional chemical catalysts and harsh conditions, but recently greener pathways with nontoxic enzymes as biocatalysts and mild conditions have shown great potential. Bacterial polyesters, such as poly(hydroxyalkanoate)s (PHA), are the best example of the biotic production of high molar mass polymers. PHAs display a wide variety of macromolecular architectures, which allow a large range of applications. The present contribution aims to provide an overview of recent progress in studies on biobased polyesters, especially those made from short building blocks, synthesized through step-growth polymerization. In addition, some important technical aspects of their syntheses through biotic or abiotic pathways have been detailed.

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“…Agro and food wastes can also be used for PHA production, eg, rice husk, wheat bran, mango peel, potato peel, bagasse, and straw (87).…”

Section: Biobased Pcmentioning

confidence: 99%

Bioplastics

Lackner

2015

Kirk-Othmer Encyclopedia of Chemical Technology

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Bioplastics are biobased polymers with two sustainability concepts: biodegradability and renewability. On the one hand, bioplastics that biodegrade to CO 2 and H 2 O in the environment can be produced, eg, avoiding litter and damage to marine organisms. On the other hand, renewable feedstocks instead of petroleum can be used, for instance, corn, sugarcane, and algae, reducing dependence on crude oil and reducing the impact on the climate. Currently, bioplastics have a market share of ∼1%, yet they experience annual growth rates in excess of 20–30%. This article highlights some key aspects associated with bioplastics, the performance of which can be tailored to meet that of petrochemical polymers or to offer new properties, eg, by blending and additivation. Important bioplastics are TPS (thermoplastic starch), PLA (polylactic acid), PHAs (polyhydroxyalkanoates), and bio‐PE, bio‐PP, and bio‐PET, which contain at least some renewable carbon.

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Agrowaste-based Polyhydroxyalkanoate (PHA) production using hydrolytic potential of Bacillus thuringiensis IAM 12077

Abstract: The study identified the innate enzymatic potential (amylase) of the PHB producing strain B.thuringiensis IAM 12077 and explored the same for cost-effective production of PHB using agrowastes, eliminating the need for pretreatment (acid hydrolysis and/or commercial enzyme

Cited by 103 publications

References 16 publications

Bioconversion of novel and renewable agro-industry by-products into a biodegradable poly(3-hydroxybutyrate) by marine Bacillus megaterium UMTKB-1 strain

Bioconversion of novel and renewable agro-industry by-products into a biodegradable poly(3-hydroxybutyrate) by marine Bacillus megaterium UMTKB-1 strain

Biotic and Abiotic Synthesis of Renewable Aliphatic Polyesters from Short Building Blocks Obtained from Biotechnology

Bioplastics

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