Modelling antagonic effect of lactic acid eacteria supernatants on some pathogenic bacteria

Portella, Augustus Caeser Franke; Karp, Susan Grace; Scheidt, Gessiel Newton; Woiciechwski, Adenise Lorenci; Parada, José; Soccol, Carlos Ricardo

doi:10.1590/s1516-89132009000700004

Cited by 14 publications

(7 citation statements)

References 36 publications

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“…In addition, broad‐spectrum antibiotics are well known to prevent the growth of other competing species and consequently alter community composition (Slattery et al ., 2001; Hibbing et al ., 2010; Tyc et al ., 2014). Some strains produce bacteriocins, which are growth‐inhibitory agents active only against closely related strains (Hirsch, 1979; Riley and Gordon, 1999; Gardner et al ., 2004; Portella et al ., 2009; Dobson et al ., 2012). Similarly to broad‐spectrum antibiotics, bacteriocins can strongly inhibit the growth of sensitive strains, change strain community composition, and additionally influence competition for host colonization (Schwinghamer and Brockwell, 1978; Hirsch, 1979; Triplett and Barta, 1987; Wilson et al ., 1998; Oresnik et al ., 1999; Riley and Gordon, 1999; Gardner et al ., 2004; Gillor et al ., 2009).…”

Section: Introductionmentioning

confidence: 99%

Genetic variation is associated with differences in facilitative and competitive interactions in the Rhizobium leguminosarum species complex

Fields

Moffat

Harrison

et al. 2021

Environmental Microbiology

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Competitive and facilitative interactions influence bacterial community composition, diversity and functioning. However, the role of genetic diversity for determining interactions between coexisting strains of the same, or closely related, species remains poorly understood. Here, we investigated the type (facilitative/inhibitory) and potential underlying mechanisms of pairwise interactions between 24 genetically diverse bacterial strains belonging to three genospecies (gsA,C,E) of the Rhizobium leguminosarum species complex. Interactions were determined indirectly, based on secreted compounds in cell-free supernatants, and directly, as growth inhibition in cocultures. We found supernatants mediated both facilitative and inhibitory interactions that varied greatly between strains and genospecies. Overall, gsE strains indirectly suppressed growth of gsA strains, while their own growth was facilitated by other genospecies' supernatants. Similar genospecies-level patterns were observed in direct competition, where gsA showed the highest susceptibility and gsE the highest inhibition capacity. At the genetic level, increased gsA susceptibility was associated with a non-random distribution of quorum sensing and secondary metabolite genes across genospecies. Together, our results suggest that genetic variation is associated with facilitative and competitive interactions, which could be important ecological mechanisms explaining R. leguminosarum diversity.

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Section: Introductionmentioning

confidence: 99%

Genetic variation is associated with differences in facilitative and competitive interactions in the Rhizobium leguminosarum species complex

Fields

Moffat

Harrison

et al. 2021

Environmental Microbiology

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“…The results of inhibitory percentage were calculated using the following equation (Portella et al . ):

I true(normal% true) = \frac{O D 620 normalc normalo normaln normalt normalr normalo normall - O D 620 normals normala normalm normalp normall normale}{O D 620 normalc normalo normaln normalt normalr normalo normall} \times 100

…”

Section: Methodsmentioning

confidence: 99%

“…This method is adapted to assay inhibitory activity in liquid Broth. The inhibitory percentage was calculated by the equation described by Portella et al (Portella et al 2009). L. pentosus TSHS was cultivated in MRS broth for 24 h at 37C.…”

Section: Bacteriocin Assaymentioning

confidence: 99%

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A Sequential Statistical Approach Towards an Optimized Production of Bacteriocin byLactobacillus pentosusTSHS

Motahari

Mirdamadi

Rad

2016

Journal of Food Processing and Preservation

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Bacteriocins, antimicrobial compounds, display applications from food preservation to medicine. The aim of this study was the statistical optimization of a bacteriocin substance production from traditional cheese bacterium taxonomically affiliated as Lactobacillus pentosus TSHS. The effects of variables were first investigated using the Placket–Burman design. Central composite design was then used to compute incubation time and NaCl concentration optimum. Response surface methodology (RSM) revealed that maximum bacteriocin production was achieved at low NaCl concentration (0.13 mol/L) and an incubation time of 47 h. The validation experiments were implemented under optimal conditions with five replicates and the bacteriocin inhibitory activity was increased by three times. The research led to parameters that maximized pentocin activity, which can be expressed as a quadratic function of incubation time and NaCl concentration. The application of RSM resulted in an enhancement in pentocin production cost effective medium. Practical Applications Bacteriocins produced by lactic acid bacteria have been found to inhibit the growth of many foodborne microorganisms, and hence, can be used as natural preservatives to increase shelf life of food. High bacteriocin production is required for application in food industry. However, bacteriocin production does not always correlate with growth rate of bacteriocin producing strain. This study has developed appropriate conditions to give optimum yield of bacteriocin. So, it revealed the possibility of bacteriocin application as biopreservatives according to industrial point of view.

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“…Alokomi et al (2000) have proved the property of lactic acid produced by Lactobacillus to increase the sensitivity of pathogens to anti-microbial substances. Other authors are of a similar opinion, stating that anti-microbial effect against gram-positive and gramnegative microorganisms by biologically active substances from lactobacteria is conditioned not only by lactic acid and hydrogen peroxide (Nomoto, 2005;Portella et al, 2009). At the same time, the synergic effect of lactic acid and protein substances is also considered possible.…”

Section: Introductionmentioning

confidence: 94%

Evaluation of anti-microbial activity of filtrates of Lactobacillus rhamnosus and Saccharomyces boulardii against antibiotic-resistant gram-negative bacteria

Isayenko

Knysh

Kotsar

et al. 2019

Regul. Mech. Biosyst.

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The article presents the results of the first study on the influence of biologically active substances Lactobacillus rhamnosus GG ATCC 53103 and Saccharomyces boulardii, obtained according to the author`s method, on growth of gram-negative bacteria with broad medical resistance: Pseudomonas aeruginosa PR, Klebsiella pneumoniae PR, Lelliottia amnigena (Enterobacter amnigenus) PR using the spectrophotometric method. Disintegrates of L. rhamnosus GG and S. boulardii were obtained using low-frequency ultrasound processing of suspension of probiotic strains, and metabolites – through cultivation of lactobacteria and saccharomycetes in disintegrates of probiotic microorganisms. To samples of test-cultures with studied filtrates of disintegrates or metabolites we added growth medium and cultivated them (period of monitoring was 5- and 24-hours). Results of the studies were expressed as the percentage of inhibition of increment in polyresistant gram-negative bacteria under the impact of biologically active substances of probiotic microorganisms. Five-hour incubation of test-strains with the studied samples of lactobacteria led to inhibition of their growth properties by 85.6–96.7%. Growth of bacteria under the impact of substances of saccharomycetes was inhibted by 45.1–92.5%. Twenty-four hour exposure of the test-cultures in filtrates of L. rhamnosus GG and S. boulardii caused 100% inhibition of P. aeruginosa and L. amnigena polyresistant strains. Temporal interval of cultivation directly proportionally affected the extent of inhibition of growth of microorganisms: we determined direct correlation dependence within 0.789–0.991. Maximum inhibition of increment of the studied pathogens was observed under the influence of metabolites of lactobacteria, obtained by cultivating primary producers in their disintegrate. We determined a high level of anti-microbial activity of metabolites from L. rhamnosus GG and S. boulardii obtained by cultivation of probiotics in disintegrates against bacteria resistant to a broad range of preparations, which allows us to consider these substances as promising for development of anti-microbial preparations of a new generation against etiologically significant antibiotic-resistant gram-negative microorganisms.

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Modelling antagonic effect of lactic acid eacteria supernatants on some pathogenic bacteria

Cited by 14 publications

References 36 publications

Genetic variation is associated with differences in facilitative and competitive interactions in the Rhizobium leguminosarum species complex

Genetic variation is associated with differences in facilitative and competitive interactions in the Rhizobium leguminosarum species complex

A Sequential Statistical Approach Towards an Optimized Production of Bacteriocin byLactobacillus pentosusTSHS

Evaluation of anti-microbial activity of filtrates of Lactobacillus rhamnosus and Saccharomyces boulardii against antibiotic-resistant gram-negative bacteria

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