Polygoni Multiflori Radix derived anthraquinones alter bile acid disposition in sandwich-cultured rat hepatocytes

Li, Kang; Si, Luqin; Rao, Jingyi; Li, Dan; Wu, Ya; Wu, Sanlan; Wu, Minghui; He, Sijie; Zhu, Weifeng; Wu, Yuping; Xu, Jiaqiang; Gao, Li; Huang, Jiangeng

doi:10.1016/j.tiv.2017.01.022

Cited by 27 publications

(22 citation statements)

References 44 publications

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“…In sandwich-cultured rat hepatocytes, chrysophanol (0, 1, 5, 25 and 50 lM) damaged liver cells by significantly inhibiting bile salt export pump and multidrug resistance-associated protein 2 to accumulate endogenous bile acids in the liver. [78] This may be related to P450 1A2, P450 2B6 and P450 3A4 in hepatocytes. These enzymes converted chrysophanol to aloe-emodin in rat and human liver microsomal incubations.…”

Section: Toxicitymentioning

confidence: 96%

“…Ohwi and Ligusticum chuanxiong hort) gave chrysophanol a higher AUC 0-t , AUC 0-∞ , t 1/2 , MRT 0-∞ and C max compared to Rheum palmatum L. group rats. [97] In addition, after Radix et Rhizoma Rhei was steamed, the content of chrysophanol increased from 0.29% to 0.39%; AUC, Rat hepatocytes Make BA accumulate in the liver 1-50 lM In vitro [78] two Salmonella strains, TA 2637 and TA 1537 Mutagenicity 1-100 lg In vitro [75] Liver cell CYP3A Toxic metabolites of naphthalene and anthraquinone bioactivation pathways 100 lg/ml In vitro [80] Liver microsomes Cause liver and kidney damage 100 lg/ml In vitro [101] Resonance light scattering spectrum Damaged DNA Unknown In vitro [73]…”

Section: Mouse Osteoblast Unknownmentioning

confidence: 99%

“…Similarly, in the primary rat hepatocytes and HepG2 cell cytotoxicity experiments, the toxicity of chrysophanol was the lowest of the five species (rhein, emodin, aloe‐emodin, physcion and chrysophanol). In sandwich‐cultured rat hepatocytes, chrysophanol (0, 1, 5, 25 and 50 μ m ) damaged liver cells by significantly inhibiting bile salt export pump and multidrug resistance‐associated protein 2 to accumulate endogenous bile acids in the liver . This may be related to P450 1A2, P450 2B6 and P450 3A4 in hepatocytes.…”

Section: Pharmacologymentioning

confidence: 99%

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Chrysophanol: a review of its pharmacology, toxicity and pharmacokinetics

Xie

Tang

Song

et al. 2019

Journal of Pharmacy and Pharmacology

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Objective Chrysophanol is a natural anthraquinone, also known as chrysophanic acid and 1,8‐dihydroxy‐3‐methyl‐anthraquinone. It has been widely used in the food and pharmaceutical fields. This review is intended to provide a comprehensive overview of the pharmacology, toxicity and pharmacokinetic researches of chrysophanol. Key finding Information on chrysophanol was collected from the Internet database PubMed, Elsevier, ResearchGate, Web of Science, Wiley Online Library and Europe PM using a combination of keywords including ‘pharmacology’, ‘toxicology’ and ‘pharmacokinetics’. The literature we collected included from January 2010 to June 2019. Chrysophanol has a wide spectrum of pharmacological effects, including anticancer, antioxidation, neuroprotection, antibacterial and antiviral, and regulating blood lipids. However, chrysophanol has obvious hepatotoxicity and nephrotoxicity, and pharmacokinetics indicate that the use of chrysophanol in combination with other drugs can reduce toxicity and enhance efficacy. Summary Chrysophanol can be used in many diseases. Future research directions include how the concentration of chrysophanol affects pharmacological effects and toxicity; the mechanism of synergy between chrysophanol and other drugs.

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Section: Toxicitymentioning

confidence: 96%

Section: Mouse Osteoblast Unknownmentioning

confidence: 99%

Section: Pharmacologymentioning

confidence: 99%

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Chrysophanol: a review of its pharmacology, toxicity and pharmacokinetics

Xie

Tang

Song

et al. 2019

Journal of Pharmacy and Pharmacology

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“…A great number of active compounds could be extracted from radix polygoni multiflori, including anthraquinones, phenolic acids, flavonoids, stilbenes, etc. Anthraquinones exhibited antimicrobial, antioxidant, antifungal, anticancer, and antihuman cytomegalovirus activities according to the papers published before , but they were found to be hepatotoxic . In addition, phenolic acids and flavonoids exerted antioxidative activity in this famous health food .…”

Section: Introductionmentioning

confidence: 85%

Quantification of eight active ingredients in crude and processed radix polygoni multiflori applying miniaturized matrix solid‐phase dispersion microextraction followed by UHPLC

Chen

et al. 2018

J of Separation Science

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A rapid, efficient, and green sample preparation method has been developed to extract eight active ingredients (gallic acid, catechins, epicatechin, polydatin, 2,3,5,4'-tetrahydroxystilbene-2-O-β-d-glucoside, resveratrol, emodin, and physcion) in radix polygoni multiflori by miniaturized matrix solid-phase dispersion microextraction. Simple and sensitive ultra high performance liquid chromatography combined with ultraviolet detection has been applied to analyze the multiple compounds. The best results were obtained by adding 25 mg sample into 25 mg adsorbent and grinding for 2 min with disorganized silica as adsorbent and 1 mL 150 mM 1-dodecyl-3-methylimidazolium bromide as a green eluting solvent. Good linearity (r > 0.998) for each analyte was obtained by this method. The intra-day and inter-day precision (RSD) were both below 5.31%, and the recoveries of the analytes ranged from 93.3 to 100.0%. This simple miniaturized matrix solid-phase dispersion microextraction method for analyzing the compounds in radix polygoni multiflori needs a short time and requires little sample and reagent. Thus, this method is far more eco-friendly and efficient than traditional extraction methods (reflux and ultrasound-assisted extraction). The present investigation provided a promising method for the fast preparation and discrimination of chemical differences in crude and processed radix polygoni multiflori.

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“…Relevant ingredients in PMR had a direct link to specific liver damage. 34,41,45) Ruling out patients with idiosyncrasies, it is difficult to find chronic liver damage caused by PMR for ordinary patients. Researchers found that PMRP has a dosetime-toxicity dependent correlation.…”

Section: Discussionmentioning

confidence: 99%

A Serum Metabolomic Study on Rats Induced by Polygoni Multiflori Radix and Polygoni Multiflori Radix Preparata by Pattern Recognition and Pathways Analysis

Zhang

Huang

Gao

2020

Biological & Pharmaceutical Bulletin

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This study focused on the differential metabolomic effects between water extracts of Polygoni Multiflori Radix and Polygoni Multiflori Radix Preparata in rats. The extracts were subsequently administered for 28 d. Serum biochemical indicators were tested, hematoxylin-eosin staining and immunohistochemistry staining were used to detect histopathological changes in the livers. Ultra-performance LC/quadrupole timeof-flight mass spectrometry was used to detect the changes in endogenous metabolites. Finally, we performed detailed analysis of the changes in metabolic pathways. Hematoxylin-eosin staining and immunohistochemistry staining results indicated that the water extracts of Polygoni Multiflori Radix and Polygoni Multiflori Radix Preparata had mild liver injury effect. Fifty-two differential endogenous biomarkers were confirmed as potential biomarkers between Polygoni Multiflori Radix and Polygoni Multiflori Radix Preparata groups. In the positive ion mode, the biomarkers included 31 Phosphatidyl cholines (PCs), six lysoPCs, and ceramide. In the negative ion mode, 12 biomarkers were confirmed, including glycodeoxycholic acid, chenodeoxycholic acid, and deoxycholic acid, etc. In Hydrophilic Interaction Liquid Chromatography (HILIC) mode, nine biomarkers were confirmed, including niacinamide, L-palmitoylcarnitine, and butyrylcarnitine, etc. Using MetaboAnalyst 4.0, six related metabolic pathways, including taurine and hypotaurine metabolism, sphingolipid metabolism, glycerophospholipid metabolism, nicotinate and nicotinamide metabolism, arginine and proline metabolism, and tryptophan metabolism and primary bile synthesis, were confirmed as the most differential pathways between the Polygoni Multiflori Radix and Polygoni Multiflori Radix Preparata groups.

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Polygoni Multiflori Radix derived anthraquinones alter bile acid disposition in sandwich-cultured rat hepatocytes

Cited by 27 publications

References 44 publications

Chrysophanol: a review of its pharmacology, toxicity and pharmacokinetics

Chrysophanol: a review of its pharmacology, toxicity and pharmacokinetics

Quantification of eight active ingredients in crude and processed radix polygoni multiflori applying miniaturized matrix solid‐phase dispersion microextraction followed by UHPLC

A Serum Metabolomic Study on Rats Induced by Polygoni Multiflori Radix and Polygoni Multiflori Radix Preparata by Pattern Recognition and Pathways Analysis

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