Poly(thymine)‐Templated Selective Formation of Fluorescent Copper Nanoparticles

Abstract

Am T‐Stück angesetzt: Poly‐T‐Einzelstrang‐DNA (blau, siehe Schema) dient als Templat für die Bildung fluoreszierender Kupfernanopartikel (CuNPs, rote Kugeln). Größe und Fluoreszenz der CuNPs sind über die Länge der Poly‐T‐Sequenz einstellbar. Andere Einzelstrang‐DNAs (grün) sind keine geeigneten Template für CuNPs und können daher zum Aufbau von Nanostrukturen mit wechselnden metallierten und nichtmetallierten Bereichen genutzt werden.

Applications of DNA Nanotechnology in Synthesis and Assembly of Inorganic Nanomaterials

“…Qing and co-workers reported a sequence-specific way to achieve the selective metallization on ssDNA. [56] The size and fluorescence of the CuNPs can be tuned just by the degree of poly-thymine (T); the size and fluorescence of these CuNPs could be tuned by the length of poly T. Liu et al [57] demonstrated that the thymine base plays a dominant role in the formation of red-emissive fluorescent CuNPs through comparing and analyzing the results with different structure-types of DNA molecules as templates as well. Song and his co-workers [58] have also researched on this aspect.…”

A fluorescent biosensor for protein detection based on poly(thymine)-templated copper nanoparticles and terminal protection of small molecule-linked DNA

“…Very recently, Qing et al found that single-stranded poly(thymine) (poly T) could also be employed as a promising template for the formation of fluorescent Cu NPs (Qing et al, 2013a). The Cu NPs formation templated by poly T was due to high affinity between thymine and Cu 2 þ , and the thymine-complexed Cu 2 þ was chemically reduced to Cu 0 by the reducing agent along the shape of the poly-T scaffold.…”

A label-free and enzyme-free ultra-sensitive transcription factors biosensor using DNA-templated copper nanoparticles as fluorescent indicator and hairpin DNA cascade reaction as signal amplifier

“…Among these biomolecules, oligonucleotide represents particularly good template candidate due to the unique nanosized structure, excellent programmable properties, and rich functional groups including heterocyclic nitrogen atoms, amino groups, and phosphate groups, which can bind with specific metal ions and provide nucleation sites for metallic nanoparticles. Nucleic acid-templated fluorescent metal nanoparticles have been intensely investigated in the past decade, such as gold nanoclusters (Au NCs) (Kennedy et al, 2012;Teng et al, 2015), silver nanoclusters (Ag NCs) (Richards et al, 2008;Enkin et al, 2014), and copper nanoparticles (Cu NPs) (Rotaru et al, 2010;Qing et al, 2013a). These DNA templated inorganic nanoparticles have the potential to be a label-free fluorescent indicator with mild reaction conditions and high efficiencies due to the fact that DNA are usually involved in the whole reaction system and strongly correlated to the target analytes.…”