A fluorescent biosensor for protein detection based on poly(thymine)-templated copper nanoparticles and terminal protection of small molecule-linked DNA

Wang, Haibo; Zhang, Hongding; Chen, Ying; Liu, Yanming

doi:10.1016/j.bios.2015.07.021

Cited by 122 publications

(19 citation statements)

References 38 publications

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“…Regarding the transduction principles, biosensors can be classified into three main class as optic [17,18,19], electrochemical [20,21,22], and piezoelectric [23,24,25,26]. Over the previous two decades, biosensors have turned out to be important to detect distinctive analytes, for example, explosives [27,28], proteins [29,30], nucleic acids [31,32], cancer biomarkers [33,34], bacteria [35,36], viruses [37,38], and toxins [39,40] in food processing [41], environmental monitoring [42], clinical diagnostics [43,44] and the fight against bioterrorism [45].…”

Section: Introductionmentioning

confidence: 99%

An Alternative Medical Diagnosis Method: Biosensors for Virus Detection

et al. 2019

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Infectious diseases still pose an omnipresent threat to global and public health, especially in many countries and rural areas of cities. Underlying reasons of such serious maladies can be summarized as the paucity of appropriate analysis methods and subsequent treatment strategies due to the limited access of centralized and equipped health care facilities for diagnosis. Biosensors hold great impact to turn our current analytical methods into diagnostic strategies by restructuring their sensing module for the detection of biomolecules, especially nano-sized objects such as protein biomarkers and viruses. Unquestionably, current sensing platforms require continuous updates to address growing challenges in the diagnosis of viruses as viruses change quickly and spread largely from person-to-person, indicating the urgency of early diagnosis. Some of the challenges can be classified in biological barriers (specificity, low number of targets, and biological matrices) and technological limitations (detection limit, linear dynamic range, stability, and reliability), as well as economical aspects that limit their implementation into resource-scarce settings. In this review, the principle and types of biosensors and their applications in the diagnosis of distinct infectious diseases were comprehensively explained. The deployment of current biosensors into resource-scarce settings is further discussed for virus detection by elaborating the pros and cons of existing methods as a conclusion and future perspective.

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Section: Introductionmentioning

confidence: 99%

An Alternative Medical Diagnosis Method: Biosensors for Virus Detection

et al. 2019

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“…SA is used in many aspects, such as biochemical sensors and nano biotechnology [18]. Therefore, we demonstrated this strategy by using a small molecule biotin and its receptor protein SA as a model example [19]. We immobilized a DNA with a designed structure and sequence on the surface of the gold electrode, and we named it M1-Biotin DNA.…”

Section: Introductionmentioning

confidence: 99%

A DNA Electrochemical Sensor via Terminal Protection of Small-Molecule-Linked DNA for Highly Sensitive Protein Detection

et al. 2021

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The qualitative and quantitative determination of marker protein is of great significance in the life sciences and in medicine. Here, we developed an electrochemical DNA biosensor for protein detection based on DNA self-assembly and the terminal protecting effects of small-molecule-linked DNA. This strategy is demonstrated using the small molecule biotin and its receptor protein streptavidin (SA). We immobilized DNA with a designed structure and sequence on the surface of the gold electrode, and we named it M1-Biotin DNA. M1-Biotin DNA selectively combines with SA to generate M1-Biotin-SA DNA and protects M1-Biotin DNA from digestion by EXO III; therefore, M1-Biotin DNA remains intact on the electrode surface. M1-Biotin-SA DNA was modified with methylene blue (MB); the MB reporter molecule is located near the surface of the gold electrode, which generates a substantial electrochemical signal during the detection of SA. Through this strategy, we can exploit the presence or absence of an electrochemical signal to provide qualitative target protein determination as well as the strength of the electrochemical signal to quantitatively analyze the target protein concentration. This strategy has been proven to be used for the quantitative analysis of the interaction between biotin and streptavidin (SA). Under optimal conditions, the detection limit of the proposed biosensor is as low as 18.8 pM, and the linear range is from 0.5 nM to 5 μM, showing high sensitivity. The detection ability of this DNA biosensor in complex serum samples has also been studied. At the same time, we detected the folate receptor (FR) to confirm that this strategy can be used to detect other proteins. Therefore, this electrochemical DNA biosensor provides a sensitive, low-cost, and fast target protein detection platform, which may provide a reliable and powerful tool for early disease diagnosis.

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“…In this experiment, DNA was used as the template to synthesize nanoparticles. DNA sequence can be designed, has good biocompatibility and unique biological recognition, and are often widely used as synthetic template [16][17][18][19][20]; specific sequences of DNA template can adjust the optical properties of metal nanoparticles and electronic structure of the DNA sequence, and the combination of nanotechnology in every field has a widespread application prospect and research value.…”

Section: Introductionmentioning

confidence: 99%

AS1411-Templated Fluorescent Cu Nanomaterial’s Synthesis and Its Application to Detecting Melamine

Wang

2020

Journal of Chemistry

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Herein, we report a novel approach to AS1411-templated formation of fluorescent copper nanomaterials and their application to melamine detection. Fluorescent copper nanomaterials were formed at room temperature by using AS1411 as a template and ascorbic acid as reductant. However, the fluorescence intensity decreased obviously in the presence of melamine. Under the optimized conditions, the quenching fluorescence intensities of copper nanomaterials showed a good linear relationship with the concentration of melamine in the range of 50 μmol/L–120 μmol/L, and the correlation coefficient was 0.9823. In addition, the method was successfully applied in the detection of melamine in milk samples. This method was cost-effective and convenient without any labels or complicated operations. Thus, this work successfully develops the capping AS1411 scaffolds of copper nanomaterials detection of melamine.

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A fluorescent biosensor for protein detection based on poly(thymine)-templated copper nanoparticles and terminal protection of small molecule-linked DNA

Cited by 122 publications

References 38 publications

An Alternative Medical Diagnosis Method: Biosensors for Virus Detection

An Alternative Medical Diagnosis Method: Biosensors for Virus Detection

A DNA Electrochemical Sensor via Terminal Protection of Small-Molecule-Linked DNA for Highly Sensitive Protein Detection

AS1411-Templated Fluorescent Cu Nanomaterial’s Synthesis and Its Application to Detecting Melamine

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