A label-free and enzyme-free ultra-sensitive transcription factors biosensor using DNA-templated copper nanoparticles as fluorescent indicator and hairpin DNA cascade reaction as signal amplifier

Luo, Sha; Zhang, Xiaojun; Wang, Guangfeng

doi:10.1016/j.bios.2016.03.066

Cited by 36 publications

(6 citation statements)

References 53 publications

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“…• Hybridization Chain Reaction (HCR)-Non-enzymatic mechanism based on successive hairpin hybridization. Applied to mRNA imaging, signal amplification and photoelectrochemical detection (Wu et al, 2015;Sha, Zhang, and Wang 2016;Chu et al, 2019).…”

Section: Blooming Contenders To the "Gold Standard" Titlementioning

confidence: 99%

Isothermal Amplification of Nucleic Acids: The Race for the Next “Gold Standard”

Veigas²,

2021

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Nucleic acid amplification technologies (NAATs) have become fundamental tools in molecular diagnostics, due to their ability to detect small amounts of target molecules. Since its development, Polymerase Chain Reaction (PCR) has been the most exploited method, being stablished as the “gold standard” technique for DNA amplification. However, the requirement for different working temperatures leads to the need of a thermocycler machine or complex thermal apparatus, which have been preventing its application in novel integrated devices for single workflow and high throughput analysis. Conversely, isothermal amplification methods have been gaining attention, especially for point-of-care diagnosis and applications. These non-PCR based methods have been developed by mimicking the in vivo amplification mechanisms, while performing the amplification with high sensitivity, selectivity and allowing for high-throughput analysis. These favorable capabilities have pushed forward the implementation and commercialization of several platforms that exploit isothermal amplification methods, mostly against virus, bacteria and other pathogens in water, food, environmental and clinical samples. Nevertheless, the future of isothermal amplification methods is still dependent on achieving technical maturity and broader commercialization of enzymes and reagents.

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Section: Blooming Contenders To the "Gold Standard" Titlementioning

confidence: 99%

Isothermal Amplification of Nucleic Acids: The Race for the Next “Gold Standard”

Veigas²,

2021

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“…An alternative non-covalent labeling strategy based on nanomaterials with appropriate optical properties can also be used. Fluorescence-based assays for the detection of nucleic acidbinding proteins can be rationally constructed using emissive nanomaterials, such as metallic nanoparticles or conjugated polymers [56,57]. The main advantage of the mentioned non-covalent labeling strategies is the facile modular assembly of the probes from readily available components.…”

Section: Fluorescence-based Methods For Studying Nucleic Acid-protein...mentioning

confidence: 99%

Environmentally sensitive fluorescent nucleoside analogues as probes for nucleic acid – protein interactions: molecular design and biosensing applications

Dziuba¹

2022

Methods Appl. Fluoresc.

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Fluorescent nucleoside analogues (FNAs) are indispensable in studying the interactions of nucleic acids with nucleic acid-binding proteins. By replacing one of the poorly emissive natural nucleosides, FNAs enable real-time optical monitoring of the binding interactions in solutions, under physiologically relevant conditions, with high sensitivity. Besides that, FNAs are widely used to probe conformational dynamics of biomolecular complexes using time-resolved fluorescence methods. Because of that, FNAs are tools of high utility for fundamental biological research, with potential applications in molecular diagnostics and drug discovery. Here I review the structural and physical factors that can be used for the conversion of the molecular binding events into a detectable fluorescence output. Typical environmentally sensitive FNAs, their properties and applications, and future challenges in the field are discussed.

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“…Apart from AuNCs and AgNCs, copper nanoclusters (CuNCs) have also been extensively used as promising fluorescence probes for detecting various targets, such as proteins, 156 single nucleotide polymorphisms (SNPs), 157 miRNAs, 158 cancer cells, 159 and so on. Jiang et al recently reported on a sensitive fluorescent biosensor for the identification of SNPs on the basis of DNA-mediated in situ synthesis of CuNC, similar to AgNCs.…”

Section: Nanoparticles As Fluorophoresmentioning

confidence: 99%

“…To overcome this problem, Xu et al 158 proposed a concatemeric dsDNA−CuNCs strategy by introducing the rolling circle replication technique in CuNP synthesis, which exhibited 4 orders of magnitude improvement in sensitivity. In addition, Sha and colleagues 156 integrated the in situ synthesis of AT-rich dsDNA−CuNCs as fluorescence reporters for hairpin DNA cascade signal amplification reaction to facilitate ultrasensitive quantification of transcription factor of nuclear factor-kappa B1 (p50). Moreover, through the introduction of enzyme-mediated substrate hydrolysis, target-induced in situ formation of DNA−CuNCs was also successfully employed for a variety of enzymes such as telomere, 160 DNA methyltransferase, 161 and nuclease.…”

Section: Nanoparticles As Fluorophoresmentioning

confidence: 99%

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Nanotechnology-Enhanced No-Wash Biosensors for in Vitro Diagnostics of Cancer

et al. 2017

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In vitro biosensors have been an integral component for early diagnosis of cancer in the clinic. Among them, no-wash biosensors, which only depend on the simple mixing of the signal generating probes and the sample solution without additional washing and separation steps, have been found to be particularly attractive. The outstanding advantages of facile, convenient, and rapid response of no-wash biosensors are especially suitable for point-of-care testing (POCT). One fast-growing field of no-wash biosensor design involves the usage of nanomaterials as signal amplification carriers or direct signal generating elements. The analytical capacity of no-wash biosensors with respect to sensitivity or limit of detection, specificity, stability, and multiplexing detection capacity is largely improved because of their large surface area, excellent optical, electrical, catalytic, and magnetic properties. This review provides a comprehensive overview of various nanomaterial-enhanced no-wash biosensing technologies and focuses on the analysis of the underlying mechanism of these technologies applied for the early detection of cancer biomarkers ranging from small molecules to proteins, and even whole cancerous cells. Representative examples are selected to demonstrate the proof-of-concept with promising applications for in vitro diagnostics of cancer. Finally, a brief discussion of common unresolved issues and a perspective outlook on the field are provided.

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A label-free and enzyme-free ultra-sensitive transcription factors biosensor using DNA-templated copper nanoparticles as fluorescent indicator and hairpin DNA cascade reaction as signal amplifier

Cited by 36 publications

References 53 publications

Isothermal Amplification of Nucleic Acids: The Race for the Next “Gold Standard”

Isothermal Amplification of Nucleic Acids: The Race for the Next “Gold Standard”

Environmentally sensitive fluorescent nucleoside analogues as probes for nucleic acid – protein interactions: molecular design and biosensing applications

Nanotechnology-Enhanced No-Wash Biosensors for in Vitro Diagnostics of Cancer

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