Poly(Hydroxybutyrate) and Norbixin as Biomaterials In Biological Applications

Abstract

This review presents the survey of performance of biomaterials prepared using norbixina and poly(hydroxybutyrate) as biomaterials in biological applications. Biomaterials can replace and/or stimulate biological systems that no longer have their functions, such as the restoration of tissue functions. In the field of biomaterials, norbixin is an antioxidant dicarboxylic carotenoid found in Bixa orellana L. and poly(hydroxybutyrate) (PHB) is a natural polymer that has characteristics of biodegradability and biocompatibility. There is a shortage of studies involving such constituents together as biomembranes in tissue healing with repair of cutaneous wounds or bone regeneration, although both present promising research that suggests its effectiveness as a guide for tissue regeneration.

Effect of norbixin-based poly(hydroxybutyrate) membranes on the tendon repair process after tenotomy in rats

“…Considering the previously reported therapeutic properties 12,16 of PHB and norbixin, which comprised the membrane used in the present study, and the rate of Achilles tendon rupture that reduces the function of the ankle joint, we decided to evaluate the efficacy of this biomaterial with respect to the inflammatory response and organization of collagen fibers at different stages during the healing process after tenotomy. According to Alves et al 21 and Monte et al 15 , biocompatible membranes are widely applied in health care to promote tissue repair; the main biological principle of this type of treatment is guided regeneration in which a membrane should favor the maintenance of stimulating factors for tissue repair, function as a cell scaffold for proliferation, and prevent the invasion of soft tissues in the injured area, thus preventing heterotrophic cell inhibition, to be considered ideal.…”

“…Studies using norbixin-based PHB membranes, which are considered easy to manufacture and associated with low cost, have shown promising results in animal models in addition to meeting adequacy and safety standards [12][13][14][15][16] . Monte et al 15 evaluated the mutagenic potential of PHB membranes through a micronucleus test and comet assay in rats using bone marrow.…”

“…New biomaterials such as biological membranes have been developed to promote tissue repair. Accordingly, several scientific studies have been carried out to analyze their interactions with biological tissues in animals and the results were promising 10,11 This has led to great progress in the total or partial repair of organs or tissues, such as in the case of norbixin-based poly(hydroxybutyrate) (PHB) membranes 12 .…”

“…Moreover, it is a biodegradable thermoplastic. Since it is biocompatible, it is easily absorbed by the human body and can thus be used to produce biological membranes to guide and stimulate repair and regeneration 12,13 .…”

“…The compounds extracted from Bixa orellana L. are widely used in many pharmaceutical, food, and cosmetic industries for their antibacterial, antifungal, and anti-inflammatory activities. Further, due to its antioxidant, antimicrobial, and antitumor properties, norbixin has great potential for use in biodegradable and biocompatible polymer membranes such as PHB 12,16,17 . Thus, considering the gap in the literature on the interaction of this biomaterial with biological tissues, the objective of this study was to evaluate the effect of norbixin-based PHB membranes on the Achilles tendon repair process after tenotomy in terms of the progression of the inflammatory process and the induction of type I and III collagen production.…”