Effect of norbixin-based poly(hydroxybutyrate) membranes on the tendon repair process after tenotomy in rats

Abstract

Purpose: To determine the efficacy of norbixin-based poly(hydroxybutyrate) (PHB) membranes for Achilles tendon repair. Methods: Thirty rats were submitted to total tenotomy surgery of the right Achilles tendon and divided into two groups (control and membrane; n = 15 each), which were further subdivided into three subgroups (days 7, 14, and 21; n = 5 each). Samples were analyzed histologically. Results: Histological analysis showed a significant reduction in inflammatory infiltrates on days 7, 14 (p < 0.0001 for both), and 21 (p = 0.0004) in the membrane group compared to that in the control group. There was also a significant decrease in the number of fibroblasts in the control group on days 7, 14 (p < 0.0001), and 21 (p = 0.0032). Further, an increase in type I collagen deposition was observed in the membrane group compared to that in the control group on days 7 (p = 0.0133) and 14 (p = 0.0107). Conclusion: Treatment with norbixin-based PHB membranes reduces the inflammatory response, increases fibroblast proliferation, and improves collagen production in the tendon repair region, especially between days 7 and 14.Effect of norbixin-based poly(hydroxybutyrate) membranes on the tendon repair process after tenotomy in rats Nascimento LDS et al. Acta Cir Bras. 2019;34(11):e201901101Effect of norbixin-based poly(hydroxybutyrate) membranes on the tendon repair process after tenotomy in rats Nascimento LDS et al. Cir Bras. 2019;34(11):e201901101 ActaEffect of norbixin-based poly(hydroxybutyrate) membranes on the tendon repair process after tenotomy in rats Nascimento LDS et al.

In vivo evaluation of bone repair guided with biological membrane based on polyhydroxybutyrate and norbixin

“…Then, in both groups, the wound margins were sutured (4-0 silk thread) for perfect coaptation. The animals received 50 mg/kg of dipyrone per gavage every 12 hr as analgesia during the entire treatment according to the protocol adopted by Alves et al 9 and Nascimento et al 17 The animals were sacrificed at 15 and 30 days after injury induction by sodium thionembutal overdose (150 mg/kg). 9,17,18 Bone tissue samples were removed from the groups, which were kept in liquid nitrogen.…”

“…[12][13][14][15][16] PHB can be associated with several biomaterials to enhance its biological properties among its most diverse applications, such as hydroxypatite, collagen, and other natural active ingredients, such as norbixin, aiming at stimulating the healing of skin wounds and bone repair, for example. [17][18][19][20][21] Norbixin, an apocarotenoid extracted from annatto (Bixa orellana L.), a plant native to Tropical America, has a great potential to be used in biodegradable and biocompatible polymeric membranes. This pigment is known to have antioxidant and antitumor properties that can support the bone repair process with a protective effect on tissue data, with potential limitation to oxidative stress and consequent abbreviation of the inflammatory process.…”

“…These associated constituents have been previously characterized physically and chemically, as well as evaluated for both genotoxicity and mutagenicity in biological systems in vivo and biocompatibility in the healing of skin wounds and tendon injuries in other related studies. 17,18,20,21 Given this perspective, taking into account the interest in understanding the interaction of biological membranes in the bone repair process, the study is justified by the development of therapeutic agents in the area of biotechnology in health and the application of new materials in the bone regeneration process. This research aims to verify the effectiveness of the PHB and norbixin membrane as scaffold in bone defects induced in the tibia of rats.…”

Effect of low intensity photobiomodulation associated with norbixin-based poly (hydroxybutyrate) membrane on post-tenotomy tendon repair. In vivo study

“…The entire surgical procedure was performed as described by Nascimento et al 19 . Initially the rats received subcutaneous injection of 0.04 mL/g of atropine for sedation and then were anesthetized intramuscularly using 10% ketamine and 2% xylazine at a dose of 0.1 mL for each 100g of body weight.…”

“…These biomaterials have biocompatible characteristics and are biodegradable, allowing total or partial regeneration of biological tissue. Within this context, a norbixin-based poly(hydroxybutyrate) (PHB) membrane was produced for use in the repair of various tissues, including tendons 19 . The PHB is a bioactive and biomimetic polymer that may originate from natural sources or be synthesized by bacteria from compounds such as butyric acid 20 – 22 .…”