Effect of low intensity photobiomodulation associated with norbixin-based poly (hydroxybutyrate) membrane on post-tenotomy tendon repair. In vivo study

Abstract

Conception, design, intellectual and scientific content of the study; acquisition and interpretation of data; technical procedures; manuscript preparation and writing. II PhD, Collaborator, Postgraduate Program in Biomedical Engineering of the Research and Development Institute, UNIVAP, Sao Jose dos Campos-SP, Brazil. Conception, design, intellectual and scientific content of the study; interpretation of data; critical revision; final approval.

In vivo evaluation of bone repair guided with biological membrane based on polyhydroxybutyrate and norbixin

“…Sousa et al 20,26 reported that the PHB/norbixin membrane proved to be nongenotoxic and biocompatible in the treatment of skin wounds induced in mice, acting as a scaffold in accelerating the healing process and shortening the initial inflammatory process. Likewise, Nascimento et al 17,18 evaluated the in vivo membrane response based on PHB and norbixin associated or not with photobiomodulation therapy with LED in the repair of tenotomized calcaneus tendon and observed that the treatment associated with LED led to a significantly more effective control of the inflammatory process than using isolated LEDs. The use of the isolated membrane optimized the production of collagen in 7 and 14 days of experiment.…”

“…The animals received 50 mg/kg of dipyrone per gavage every 12 hr as analgesia during the entire treatment according to the protocol adopted by Alves et al 9 and Nascimento et al 17 The animals were sacrificed at 15 and 30 days after injury induction by sodium thionembutal overdose (150 mg/kg). 9,17,18 Bone tissue samples were removed from the groups, which were kept in liquid nitrogen. Then, a sample from each group underwent SEM analysis.…”

“…PHB can be associated with several biomaterials to enhance its biological properties among its most diverse applications, such as hydroxypatite, collagen, and other natural active ingredients, such as norbixin, aiming at stimulating the healing of skin wounds and bone repair, for example 17‐21 …”

“…[12][13][14][15][16] PHB can be associated with several biomaterials to enhance its biological properties among its most diverse applications, such as hydroxypatite, collagen, and other natural active ingredients, such as norbixin, aiming at stimulating the healing of skin wounds and bone repair, for example. [17][18][19][20][21] Norbixin, an apocarotenoid extracted from annatto (Bixa orellana L.), a plant native to Tropical America, has a great potential to be used in biodegradable and biocompatible polymeric membranes. This pigment is known to have antioxidant and antitumor properties that can support the bone repair process with a protective effect on tissue data, with potential limitation to oxidative stress and consequent abbreviation of the inflammatory process.…”

“…These associated constituents have been previously characterized physically and chemically, as well as evaluated for both genotoxicity and mutagenicity in biological systems in vivo and biocompatibility in the healing of skin wounds and tendon injuries in other related studies. 17,18,20,21 Given this perspective, taking into account the interest in understanding the interaction of biological membranes in the bone repair process, the study is justified by the development of therapeutic agents in the area of biotechnology in health and the application of new materials in the bone regeneration process. This research aims to verify the effectiveness of the PHB and norbixin membrane as scaffold in bone defects induced in the tibia of rats.…”