Poly(ethylene glycol) as stabilizer and emulsifying agent: a novel stabilization approach preventing aggregation and inactivation of proteins upon encapsulation in bioerodible polyester microspheres

Optimized stability retention of a monoclonal antibody in the PLGA nanoparticles

“…Although PEG is a hydrophilic polymer used as stabilizing agent, our results did not suggest any effects on stability and activity. It was reported that PEG prevented structural perturbation of protein because of preventing protein adsorption on the O/W interface (Castellanos et al, 2003).…”

Biodegradable Poly(D,L-lactic-co-glycolic acid)-Based Micro/Nanoparticles for Sustained Release of Protein Drugs - A Review

“…To protect protein from denaturation during formation of W/O emulsion, S/O/W method has been developed. This is because, proteins in the solid state are believed to maintain their bioactivity by drastically reducing conformational change in comparison to the large structural change found in the dissolved state [56]. In the S/O/W method, dehydrated protein powders are dispersed in the polymer solution to form the primary emulsion.…”

New Insight into the Role of Polyethylene Glycol Acting as Protein Release Modifier in Lipidic Implants

“…The major cause of protein denaturation occurring during this type of preparation techniques is the exposure of the macromolecules to the aqueousorganic interfaces. As also PEG 400 adsorbs to these interfaces, it competes with the proteins and, thus, protects them from degradation (22,23).…”