Optimized stability retention of a monoclonal antibody in the PLGA nanoparticles

Son, Sejin; Lee, William R.; Joung, Yoon Ki; Kwon, Myung Hee; Kim, Yong‐Sung; Парк, Ки Донг

doi:10.1016/j.ijpharm.2008.09.061

Cited by 40 publications

(14 citation statements)

References 36 publications

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“…28, 32 For example, G4-RHO-FA-OH (100 μL, 1 mg/mL in ddH 2 O) was added to 20 mg of either PEG5K-PLA23K, PEG5K-PLA30K, or PEG5K-PLA45K in 1 mL of DCM, and the mixture was sonicated for 1 min using a Misonix XL Ultrasonic Processor (100% duty cycle, 475 W, 1/8″ tip, QSonica, LLC, Newtown, CT). Two milliliters of 3% aqueous PVA solution was then added to the mixture, followed by additional sonication for 1 min.…”

Section: Methodsmentioning

confidence: 99%

Temporal Control over Cellular Targeting through Hybridization of Folate-targeted Dendrimers and PEG-PLA Nanoparticles

Sunoqrot¹,

Bae²,

Pearson³

et al. 2012

Biomacromolecules

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Polymeric nanoparticles (NPs) and dendrimers are two major classes of nanomaterials that have demonstrated great potential for targeted drug delivery. However, their targeting efficacy has not yet met clinical needs largely because of a lack of control over their targeting kinetics, which often results in rapid clearance and off-target drug delivery. To address this issue, we have designed a novel hybrid NP (nanohybrid) platform that allows targeting kinetics to be effectively controlled through hybridization of targeted dendrimers with polymeric NPs. Folate (FA)-targeted generation 4 poly(amidoamine) dendrimers were encapsulated into poly(ethylene glycol)-b-poly(D,L-lactide) (PEG-PLA) NPs using a double emulsion method, forming nanohybrids with a uniform size (~100 nm in diameter) at high encapsulation efficiencies (69–85%). Targeted dendrimers encapsulated within the NPs selectively interacted with FA receptor (FR)-overexpressing KB cells upon release in a temporally controlled manner. The targeting kinetics of the nanohybrids were modulated using three different molecular weights (MW) of the PLA block (23, 30, and 45 kDa). The release rates of the dendrimers from the nanohybrids were inversely proportional to the MW of the PLA block, which dictated their binding and internalization kinetics with KB cells. Our results provide evidence that selective cellular interactions can be kinetically controlled by the nanohybrid design, which can potentially enhance targeting efficacy of nanocarriers.

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Section: Methodsmentioning

confidence: 99%

Temporal Control over Cellular Targeting through Hybridization of Folate-targeted Dendrimers and PEG-PLA Nanoparticles

Sunoqrot¹,

Bae²,

Pearson³

et al. 2012

Biomacromolecules

View full text Add to dashboard Cite

show abstract

“…Stability problems are major issues in drug products/nanomedicines incorporated with biotechnological products [26]. Most nano medicines incorporated with biotechnological products need precisely defined storage conditions, which mainly depend upon the biotechnological product (owing to their stability problems) [12].…”

Section: Pharmaceutical Stability Testingmentioning

confidence: 99%