New Insight into the Role of Polyethylene Glycol Acting as Protein Release Modifier in Lipidic Implants

Abstract

For the first time it is shown that the release of pharmaceutical proteins can be controlled by an in-situ precipitation within inert matrices.

Basic Chromatographic Properties of Polyethylene Glycol-type, Polymer-based Monolithic Columns

“…There was the plot of phenol (3) on the line. Further parallel shift of line shown as shadow area (d) covered the plots of m-, p-phthalic acids (6,7), m-, p-hydroxybenzoic acids (9,10), and m-, p-benzene diols (12,13). Retentions of these solutes having two substituted carboxyl groups and/or phenolic hydroxyl groups added further contributions of carboxyl groups and phenolic hydroxyl groups to the retentions of benzoic acid and phenol.…”

“…For example, PEG was applied as a precipitating agent for crystallization of proteins, 1 a filter for osmotic ultrafiltration, 2 DNA introduction into cell 3 and cell fusion reagent, 4 and in other researches in biology. [5][6][7] PEG was also useful as separation media for protein analysis 8,9 because PEG does not specifically interact with biomaterials. Based on these results, we have applied PEG-based polymers for new affinity resins to improve the searching target protein.…”

Implants as Sustained Release Delivery Devices for Vaccine Antigens

“…The extracted protein is commonly run on a polyacrylamide gel and protein integrity is analyzed by comparison to a standard (Mohl and Winter 2004 ;Herrmann et al 2007a ). Additionally occurring bands, or disappearance of bands may be a hint that protein integrity has been compromised.…”

Release pathways of interferon α2a molecules from lipid twin screw extrudates revealed by single molecule fluorescence microscopy

“…IFNα was lyophilized as described by Herrmann and Mohl [28]. In brief, HP-β-CD and IFNα were mixed in a ratio of 3:1 (m/m) and spiked with a dilution of freshly labeled fluo-IFNα.…”