Poly 3-(hydroxyalkanoates) produced from oily substrates by Pseudomonas aeruginosa 47T2 (NCBIM 40044): Effect of nutrients and incubation temperature on polymer composition

Haba, E.; Vidal-Mas, Jaume; Bassas, M.; Espuny, M. J.; Llorens, Joan; Manresa, Ángeles

doi:10.1016/j.bej.2006.11.021

Cited by 83 publications

(52 citation statements)

References 37 publications

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“…Using hydrophilic substrates, such as glycerol and CW, the PHA yield varied between 1.2 and 22.2% of the CDW, which is in agreement with results reported in the literature in which higher yields of PHA were obtained with hydrophobic substrates, such as WCO and other oily wastes, than with hydrophilic substrates. In some cases, the use of hydrophobic substances resulted in the production of up to 54.6% PHA of the CDW [9,12,20]. Using CWO, we obtained a 39% CDW yield with strain L2-1.…”

Section: Resultsmentioning

confidence: 92%

“…The shift to shorter chain length monomers and the increase in the proportion of unsaturated monomers observed in medium containing WCO compared to those containing water-soluble carbon sources are in agreement with the results reported by others. Haba et al [12] and Marsudi et al [20], working with other P. aeruginosa strains grown in vegetable oils or oily wastes, observed PHA compositions of C 10:0 (43-48%), C 12:0 (11-15%) and C 8:0 (10-38%), with a relatively high proportion of unsaturated monomers (5-15%). The origin of these unsaturated monomers was studied by Tan et al [30] in P. putida PGA1, using saponiWed palm kernel oil (SPKO) (a mixture of saturated and unsaturated fatty acids), myristic acid (a saturated fatty acid), lauric acid (a saturated fatty acid) and oleic acid (an unsaturated fatty acid).…”

Section: Characterization Of Pha Monomersmentioning

confidence: 96%

“…With the aim of improving the economics of the production process and obtaining new compounds with diVerent physical and chemical properties, various agroindustrial by-products or wastes have been evaluated as substrates for the production of rhamnolipids and PHAs. In particular, waste cooking oil (WCO) [12], molasses [25], vegetable oils and residues from vegetable oil reWneries [21] have been shown to be excellent substrates for rhamnolipids and PHAs production. Cassava wastewater (CW) is a carbohydrate-rich residue generated in large amounts during the production of cassava Xour.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Cassava wastewater as a substrate for the simultaneous production of rhamnolipids and polyhydroxyalkanoates by Pseudomonas aeruginosa

Costa

Lépine

Milot

et al. 2009

J Ind Microbiol Biotechnol

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Glycerol, cassava wastewater (CW), waste cooking oil and CW with waste frying oils were evaluated as alternative low-cost carbon substrates for the production of rhamnolipids and polyhydroxyalkanoates (PHAs) by various Pseudomonas aeruginosa strains. The polymers and surfactants produced were characterized by gas chromatography-mass spectrophotometry (MS) and by high-performance liquid chromatography-MS, and their composition was found to vary with the carbon source and the strain used in the fermentation. The best overall production of rhamnolipids and PHAs was obtained with CW with frying oil as the carbon source, with PHA production corresponding to 39% of the cell dry weight and rhamnolipid production being 660 mg l(-1). Under these conditions, the surface tension of the culture decreased to 30 mN m(-1), and the critical micelle concentration was 26.5 mg l(-1). It would appear that CW with frying oil has the highest potential as an alternative substrate, and its use may contribute to a reduction in the overall environmental impact generated by discarding such residues.

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Section: Resultsmentioning

confidence: 92%

Section: Characterization Of Pha Monomersmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Cassava wastewater as a substrate for the simultaneous production of rhamnolipids and polyhydroxyalkanoates by Pseudomonas aeruginosa

Costa

Lépine

Milot

et al. 2009

J Ind Microbiol Biotechnol

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“…P. aeruginosa is a common bacterium that can be isolated from many natural and artificial environments, probably due to its capacity to grow in low nutrient environments (Legnani et al, 1999). This organism is a representative bacterium capable of producing MCL-PHAs when alkanoic acids or oils are provided as a carbon substrate (Chan et al, 2006;Haba et al, 2007). In the present study, the predominance of P. aeruginosa strains is considered to be related to their high cell growth rate with their high PHA storage capacity during the enrichment.…”

Section: Dgge Analyses Of the Microbial Communitymentioning

confidence: 95%

Production of medium-chain-length polyhydroxyalkanoates by activated sludge enriched under periodic feeding with nonanoic acid

Lee

Kim

Mishra

et al. 2011

Bioresource Technology

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“…aeruginosa has been reported for the production of biosurfactant when growing in water immiscible substrate (Hori et al, 2002;Haba et al, 2007). Studies were carried out to obtain the production of mcl-PHB from Pseudomonas species.…”

Section: Effect Of Incubation Time On Phb Productionmentioning

confidence: 99%

Bioplastic (Poly -3-Hydroxybutyrate) Production by Local Pseudomonas Aeruginosa Isolates Utilizing Waste Cooking Oil

Gatea¹

2017

WJPR

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Disposal of domestic wastes, such as waste cooking oil (WCO), without pretreatment, contributes to the deterioration of the environment and creating severs problems. Synthetic plastic poses threat to the environment, because it is non-biodegradable are now accumulate in the environment at great millions of tons per year.Bioplastic are polymers such as Poly -3-hydroxybutyrate (PHB), which has gained importance since it can be easily degrade in nature. The present study was aimed to isolate a promising microbial producer of (PHB), and optimization experiment to evaluate the best environmental and physiological factors that lead to maximum (PHB) production.Seventy Pseudomonas species were isolated from different contaminated soil and water samples. The isolates thereafter were screened for (PHB) production using Sudan black and Nile blue as indicators stains to investigate the bacterial isolates for PHB production. It was found that 50 of them were capable of producing PHB.The isolate Pseudomonas aeruginosa Dw 7 exhibit higher production of PHB, therefore it's selected for further studies. The isolate DW7 is definitively identified as Pseudomonas aeruginosa Dw7 after complete 16S rRNA gene sequences method. Highest accumulation of PHB and DCW were at 30°C, recorded 0.65 g/L and 1.78 g/l respectively with deletion of surface tension to 28mN/m with yield of PHB ranged between (35.6 and 36.5 %). Different WCO were tested as the sole carbon sources. The highest cell growth was observed on the polymer samples produced from corn oil, followed by the polymer produced using sunflower oil. The isolate yielded a relatively good dry weight 1.78 g/l with 0.72 of PHB yielding 40%

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Poly 3-(hydroxyalkanoates) produced from oily substrates by Pseudomonas aeruginosa 47T2 (NCBIM 40044): Effect of nutrients and incubation temperature on polymer composition

Cited by 83 publications

References 37 publications

Cassava wastewater as a substrate for the simultaneous production of rhamnolipids and polyhydroxyalkanoates by Pseudomonas aeruginosa

Cassava wastewater as a substrate for the simultaneous production of rhamnolipids and polyhydroxyalkanoates by Pseudomonas aeruginosa

Production of medium-chain-length polyhydroxyalkanoates by activated sludge enriched under periodic feeding with nonanoic acid

Bioplastic (Poly -3-Hydroxybutyrate) Production by Local Pseudomonas Aeruginosa Isolates Utilizing Waste Cooking Oil

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