Twenty seven hydrocarbon degrading bacterial isolates were isolated from five hydrocarbon contaminated sites. The study revealed a high efficiency of bacteria adapted to the biodegradation of hydrocarbons (petroleum) isolated from soil contaminated with oil residues. The isolates were examined for their hydrocarbon degradation in media supplemented with crude oil at five different concentrations 2% 3% 5%, 7% and 10% incubated for 5 different time intervals 5, 10, 15, 20, and 30 days. The results indicated that all the isolates possessed potential to degrade the wide variety of hydrocarbons. The most efficient among them was SD1 which degraded most tested hydrocarbon (98%) showing maximum growth at 3.3 gm/l of biomass concentration and 15 days incubation. SD1 isolate was identified on the basis of morphological and biochemical characteristics and confirmed with 16s rRNA sequencing. GCMS Analysis showed significant differences in the composition of hydrocarbons in Crude oil. It could be concluded that native flora of hydrocarbon contaminated site adapt to the environmental condition and could be implicated to remove hydrocarbons.
The research aimed to obtain new bacterial isolate producing cellulase enzyme, and ultrasonic effect on enzyme activity. Three bacterial isolates were isolated from different agricultural wastes samples. Their effectiveness in cellulolytic was detected by measurement of the clear zone diameter around bacterial growth. The enzyme was extracted after the growtht of local bacterial isolate which has highest enzyme activity. The enzyme was exposed to ultrasound intensity of 40 KH, low, medium and high power, and exposure time (10, 20, 30, 40, 50, and 60) min, enzymatic activity was measured after each treatment compared with control. It was found at low level that an increase in the cellulase activity with increased ultrasonic exposure time, the higher activity at 60(min). The effect of ultrasonic treatment at mid-level, showed higher activity at 20 (min), while at high level the activity decrease with exposure time when compared with control. Protein concentration was estimated, there was no significant difference on protein content after ultrasonic treatment in samples at (10, 20, 30, 40) min while decrease in protein content in samples at (50, 60) min, compared with with control.
Silver nanoparticles were synthesized using different aqueous concentrations of silver nitrate (0.5,1, 2, 3) with olive leaf extract as reductive factor. The reaction showed a strong change in color from yellow to dark brown. Antimicrobial activity against pathogenic bacteria like Staph aureus, E. coli was studied. Spectral properties were studied by using UV- visible spectrophotometer, all concentrations shown peaks at (435)nm. The nanosilver particles tested by Dynamic light scattering (DLS) so the size particles were about (80-126)nm, zeta potential values were (-17 - -23)m V. Biological silver nanoparticles gained interest in recent past owing to its simple preparation, low cost, ecofriendly and their products more stability.
Disposal of domestic wastes, such as waste cooking oil (WCO), without pretreatment, contributes to the deterioration of the environment and creating severs problems. Synthetic plastic poses threat to the environment, because it is non-biodegradable are now accumulate in the environment at great millions of tons per year.Bioplastic are polymers such as Poly -3-hydroxybutyrate (PHB), which has gained importance since it can be easily degrade in nature. The present study was aimed to isolate a promising microbial producer of (PHB), and optimization experiment to evaluate the best environmental and physiological factors that lead to maximum (PHB) production.Seventy Pseudomonas species were isolated from different contaminated soil and water samples. The isolates thereafter were screened for (PHB) production using Sudan black and Nile blue as indicators stains to investigate the bacterial isolates for PHB production. It was found that 50 of them were capable of producing PHB.The isolate Pseudomonas aeruginosa Dw 7 exhibit higher production of PHB, therefore it's selected for further studies. The isolate DW7 is definitively identified as Pseudomonas aeruginosa Dw7 after complete 16S rRNA gene sequences method. Highest accumulation of PHB and DCW were at 30°C, recorded 0.65 g/L and 1.78 g/l respectively with deletion of surface tension to 28mN/m with yield of PHB ranged between (35.6 and 36.5 %). Different WCO were tested as the sole carbon sources. The highest cell growth was observed on the polymer samples produced from corn oil, followed by the polymer produced using sunflower oil. The isolate yielded a relatively good dry weight 1.78 g/l with 0.72 of PHB yielding 40%
Some pesticides are readily degraded by microorganisms; others have proven to be recalcitrant. A diverse group of bacteria, including members of the genera Alcaligenes, Flavobacterium, Pseudomonas and Rhodococcus, metabolize pesticides. Microbial degradation depends not only on the presence of microbes with the appropriate degradative enzymes, but also on a wide range of environmental parameters. In this research two bacterial isolates defined consortium with Propachlor biodegradation capacity, were isolated from soil samples from pesticides contaminated site. These consortia: Streptomyces sp.Y7andRhodococcus sp.S3 produced a significant increment of the specific degradation activity compared to the individual culture with 20 mg L-1 of Propachlor as the only carbon source, consortium of Streptomyces spY7 and Rhodococcus spS3 were able to grow with different concentration of Propachlor. The microorganisms present in the special growth medium provided with Propachlor as a sole carbon source were found to possess the ability to degrade 84% of it after 48 h. of incubation at 30°C with shaking at 120 rpm and optimum pH was7 and 35 mg L-1of pesticide was the favorable concentration. Therefore the selected bacterial isolates could be considered one of the most promising bacterial groups for COP biodegradation in contaminated environment.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.