Cassava wastewater as a substrate for the simultaneous production of rhamnolipids and polyhydroxyalkanoates by Pseudomonas aeruginosa

Abstract

Glycerol, cassava wastewater (CW), waste cooking oil and CW with waste frying oils were evaluated as alternative low-cost carbon substrates for the production of rhamnolipids and polyhydroxyalkanoates (PHAs) by various Pseudomonas aeruginosa strains. The polymers and surfactants produced were characterized by gas chromatography-mass spectrophotometry (MS) and by high-performance liquid chromatography-MS, and their composition was found to vary with the carbon source and the strain used in the fermentation. The best overall production of rhamnolipids and PHAs was obtained with CW with frying oil as the carbon source, with PHA production corresponding to 39% of the cell dry weight and rhamnolipid production being 660 mg l(-1). Under these conditions, the surface tension of the culture decreased to 30 mN m(-1), and the critical micelle concentration was 26.5 mg l(-1). It would appear that CW with frying oil has the highest potential as an alternative substrate, and its use may contribute to a reduction in the overall environmental impact generated by discarding such residues.

Production of medium-chain-length polyhydroxyalkanoate by Pseudomonas oleovorans grown in sugary cassava extract supplemented with andiroba oil

“…Co-polymers with short-and medium-chain-length monomeric units were produced mainly when the sugary cassava extract was supplemented with andiroba oil. In studies using other strains of Pseudomonas and mixtures of lipids and carbohydrates as carbon sources, Ashby et al (2001) and Costa et al (2009) did not detect production of these copolymers. On the other hand, Srivastava & Tripathi (2013) observed production of PHAs with short-and medium-chain-length in a study using Alcaligenes sp.…”

“…oleovorans shows the specific ability to synthesize mediumchain-length PHAs (monomers with 6 to 16 carbon atoms in the main chain) from fatty acids (β-oxidation) (Fiedler et al, 2002), and sugars (de novo biosynthesis) (Huisman et al, 1989). That ability has encouraged the use of different combinations of carbohydrates and lipids to produce biopolymers with particular properties and function; such combinations include the glucose/lipid mixture (Ashby et al, 2001), hydrolyzed corn oil (Shang et al, 2008), cassava wastewater (Costa et al, 2009), whey and starch sources (Steinbüchel, 2010), and palm oil (Sudesh et al, 2011).…”

“…Ramadas et al (2009) and Aremu et al (2010) produced PHAs from enzymatically and thermally hydrolyzed cassava starch. Using cassava wastewater, Nitschke & Pastore (2006) produced biosurfactants, and Costa et al (2009) produced PHAs and rhamnolipids.…”

Improvement of L(+)-lactic acid production from cassava wastewater by Lactobacillus rhamnosus B 103

“…13 Cassava wastewater has been used to produce a surfactin by Bacillus subtilis, 14 citric acid by Aspergillusniger, 15 Polyhydroxyalkanoates (PHAs) and rhamnolipids by Pseudomonas aeruginosa. 16 Some studies in the literature report the use of cassava bagasse in the production of lactic acid, 17 -21 but no papers were found on lactic acid production using cassava wastewater as a substrate.…”

PRODUÇÃO DE BIOTENSOATIVO UTILIZANDO Pseudomonas aeruginosa (P.A.) E RESÍDUO AGROINDUSTRIAL (MANIPUEIRA) COMO SUBSTRATO

“…A manipueira é o efluente resultante da industrialização da mandioca, decorrente da prensagem da mandioca ralada e lavada no processo da obtenção da farinha (Costa et al, 2009), possuindo um alto teor de matéria orgânica e um glicosídio chamado linamarina que é facilmente hidrolisado a cianeto, composto altamente tóxico ao metabolismo, sendo na maioria das vezes descartada in natura nos cursos d'água acarretando um grande problema ambiental (Costa et al, 2009;Cordeiro, 2006). Apesar de altamente poluente, a manipueira pode ser empregada como matéria-prima para outros processos industriais, por apresentar altas concentrações de carboidratos, nitrogênio e sais minerais.…”