PRODUÇÃO DE BIOTENSOATIVO UTILIZANDO Pseudomonas aeruginosa (P.A.) E RESÍDUO AGROINDUSTRIAL (MANIPUEIRA) COMO SUBSTRATO

Abstract

BIOTENSOATIVE PRODUCTION USING Pseudomonas aeruginosa (P.A.) AND AGROINDUSTRY WASTE (CASSAVA WASTEWATER) AS SUBSTRACT ABSTRACTBiosurfactants are amphoteric molecules that act preferentially on the interface of fluids of different polarities such as water and oil, and they may be synthesized by micro-organisms. Using a factorial design 2 4-1 (half fraction) 10 assays were performed using Pseudomonas aeruginosa (PA) AP029/GVII-A as microorganism in rotary incubator (shaker), in which we analyzed the influence of factors (temperature, agitation, rate of aeration and concentration of culture medium) at two different levels for the synthesis of biosurfactant. Samples were collected throughout the cultivation until we completed 132h of fermentation. We tried the best result following the production through consumption of substrate, dry matter, reduction of surface tension (ring method) and emulsification index. The results showed that the cassava wastewater is a very assimilable substrate for the production of biosurfactant, reaching 91% of consumption by the micro-organism under study. The cultivation temperature was found to be one of the leading factors in the synthesis of the metabolite, followed by aeration rate and also due to the agitation.The best results showed a reduction of 30% in surface tension (% RTS) for the medium, reaching values of 30 mN/m, 3.0 g / L of biomass and emulsification index greater than 65%.

Produção de biossurfactante por Chromobacterium violaceum ATCC 12472 utilizando milhocina e óleo de milho pós-fritura como nutrientes

“…2). Rocha et al (2006;2007) obtiveram resultados semelhantes de 71,79% de emulsificação para óleo de soja e inferiores de 16,50% de querosene, respectivamente, utilizando Pseudomonas aeruginosa em meio de suco de caju; enquanto Bezerra et al (2012) encontraram valores em torno de 64,29% utilizando querosene comercial com o mesmo micro-organismo. Barbosa e Paz (2007) utilizaram a C. violaceum e encontraram valores inferiores (43,30%) de emulsificação no período de 60 horas após 2 minutos utilizando óleo de pequi.…”

<b>Biosurfactants produced by <i>Microbacterium</i> sp., isolated from aquatic macrophytes in hydrocarbon-contaminated area in the Rio Negro, Manaus, Amazonas

“…To evaluate the biosurfactant production over time, a production curve was obtained (Figure 1) following method described by Bezerra et al (2012). The production curve was plotted from the stationary phase, starting within 48 hours and remaining constant for up to 96 hours, this procedure was also followed by Pinto, Martins, and Costa, (2009) when using different microorganisms in mixed cultures of Corynebacterium aquaticum and Bacillus sp.…”

“…are the most representative (Bezerra, Holanda, Amorim, Macedo, & Santos, 2012;Decesaro, Rigon, Thomé, & Colla, 2013).…”

Adsorption of copper, zinc and lead on biosurfactant produced from cassava wastewater

“…Biosurfactant production in industrial scale is usually limited by the high cost of culture media, associated with inefficient downstream methods and to relatively low incomes of the product (Henkel et al, 2012). The use of agroindustrial wastes as substrates for microbial production has been suggested as an alternative to make the process commercially feasible (Colla and Costa, 2003;Barros et al, 2007;Bezerra et al, 2012;Henkel et al, 2012) and enables, as well, the utilization of million tons of environmentally dangerous residues in biotechnological processes (Cortés-Camargo et al, 2016).…”

“…The high concentration of remaining carbohydrates and several important micronutrients can cause a serious problem of environmental pollution if the effluent is not properly treated before disposition (Damasceno et al, 2003). On the other hand, this is also an interesting characteristic in a culture medium, since there might be no need for supplementation to biosurfactant production Kummer et al 111 (Barros et al, 2007;Bezerra et al, 2012). The application of surfactants in order to remediate soils contaminated with hydrocarbons and/or heavy metals has been widely studied and the biosurfactants are an attractive option because of their biodegradability and lower toxicity (Song et al, 2008;Wan et al, 2011).…”