Production of medium-chain-length polyhydroxyalkanoates by activated sludge enriched under periodic feeding with nonanoic acid

Lee, Sun Hee; Kim, Jae Hee; Mishra, Dhaneshwar; Ni, Yu-Yang; Rhee, Young Ha

doi:10.1016/j.biortech.2011.03.025

Cited by 25 publications

(23 citation statements)

References 32 publications

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“…It has recently been reported that MCL-PHA-accumulating organisms can be enriched by periodic feeding of nonanoic acid in a SBR (Lee et al, 2011). In this study, several microorganisms that were predominant in the bacterial community under these dynamic conditions were isolated and screened for MCL-PHA production.…”

Section: Resultsmentioning

confidence: 99%

“…A PHA-producing bacterial strain, P-5, was isolated from a waste-activated sludge sample that was enriched by periodic feeding with nonanoic acid in a sequencing batch reactor (SBR) (Lee et al, 2011). The phenotypic characteristics of the isolate were analyzed using VITEK ® 2 Compact (bioMérieux, France).…”

Section: Isolation and Identification Of A Pha-producing Microorganismmentioning

confidence: 99%

“…PHAs were extracted from lyophilized cells by hot chloroform extraction using a Soxhlet apparatus and purified by precipitation with methanol (Lee et al, 2011). The relative amounts of PHA monomer units were calculated from the area of the peaks of the methyl-esters of each monomer unit on gas chromatograms of the methanolyzed samples.…”

Section: Isolation and Analysis Of Phasmentioning

confidence: 99%

“…The phbC-F and phbC-R primers (Lee et al, 2011) and the I-179L and I-179R primers (Solaiman et al, 2000) were used to amplify the phbC and phaC genes, respectively. PCR was performed using two sets of primers to clone complete genes encoding PhaC1 and PhaC2 from P. aeruginosa P-5 genomic DNA.…”

Section: Molecular Analysis Of Pha Synthase Genesmentioning

confidence: 99%

“…This might be attributable to the difference in carbon availability of the P-5 isolate for nonanoic acid and valeric acid. Among various alkanoic acids, nonanoic acid and octanoic acid are well-known carbon substrates for the efficient production of PHAs by many pseudomonads (Kim et al, 2007;Lee et al, 2011). However, the consumption rate of valeric acid was much lower than that of nonanoic acid, which resulted in no noticeable increase in DCW or PHA content after the depletion of nonanoic acid.…”

Section: Effect Of Valerate As the 3hv Precursormentioning

confidence: 99%

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Biosynthesis of Copolyesters Consisting of 3-Hydroxyvalerate and Medium-chain-length 3-hydroxyalkanoates by the Pseudomonas aeruginosa P-5 Strain

Woo¹,

Kim²,

Ni³

et al. 2012

The Korean Journal of Microbiology

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A bacterial strain capable of synthesizing polyhydroxyalkanoates (PHAs) with an unusual pattern of monomer units was isolated from activated sludge using the enrichment culture technique. The organism, identified as Pseudomonas aeruginosa P-5, produced polyesters consisting of 3-hydroxyvalerate and medium-chain-length (MCL) 3-hydroxyalkanoate monomer units when C-odd alkanoic acids such as nonanoic acid and heptanoic acid were fed as the sole carbon source. Solvent fractionation experiments using chloroform and hexane revealed that the 3-hydroxyalkanoate monomer units in these polyesters were copolymerized. The molar concentration of 3-hydroxyvalerate in the polyesters produced were significantly elevated up to 26 mol% by adding 1.0 g/L valeric acid as the cosubstrate. These copolyesters were sticky with low degrees of crystallinity. The PHA synthase genes were cloned, and the deduced amino acid sequences were determined. P. aeruginosa P-5 possessed genes encoding MCL-PHA synthases (PhaC1 and PhaC2) but lacked the short-chain-length PHA synthase gene, suggesting that the MCL-PHA synthases from P. aeruginosa P-5 are uniquely active for polymerizing (R)-3-hydroxyvaleryl-CoA as well as MCL (R)-3-hydroxyacyl-CoAs.

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Section: Resultsmentioning

confidence: 99%

Section: Isolation and Identification Of A Pha-producing Microorganismmentioning

confidence: 99%

Section: Isolation and Analysis Of Phasmentioning

confidence: 99%

Section: Molecular Analysis Of Pha Synthase Genesmentioning

confidence: 99%

Section: Effect Of Valerate As the 3hv Precursormentioning

confidence: 99%

See 3 more Smart Citations

Biosynthesis of Copolyesters Consisting of 3-Hydroxyvalerate and Medium-chain-length 3-hydroxyalkanoates by the Pseudomonas aeruginosa P-5 Strain

Woo¹,

Kim²,

Ni³

et al. 2012

The Korean Journal of Microbiology

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Production of medium‐chain‐length PHA in octanoate‐fed enrichments dominated by Sphaerotilus sp.

Vermeer

Depaz

Berg

et al. 2022

Biotech & Bioengineering

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Medium-chain-length polyhydroxyalkanoate (mcl-PHA) production by using microbial enrichments is a promising but largely unexplored approach to obtain elastomeric biomaterials from secondary resources. In this study, several enrichment strategies were tested to select a community with a high mcl-PHA storage capacity when feeding octanoate. On the basis of analysis of the metabolic pathways, the hypothesis was formulated that mcl-PHA production is more favorable under oxygen-limited conditions than short-chain-length PHA (scl-PHA). This hypothesis was confirmed by bioreactor experiments showing that oxygen limitation during the PHA accumulation experiments resulted in a higher fraction of mcl-PHA over scl-PHA (i.e., a PHA content

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Semi‐continuous non‐sterile production of medium chain‐length polyhydroxyalkanoates from fatty acids

Blunt,

Lagassé,

Harvey

et al. 2024

Can J Chem Eng

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Medium chain‐length polyhydroxyalkanoates (mcl‐PHAs) are efficiently synthesized from fatty acids. It was hypothesized that under non‐axenic conditions, the anti‐microbial properties of fatty acids would reduce growth of microbial competitors and allow Pseudomonas putida LS46 to dominate the population resulting in non‐sterile mcl‐PHA production. Non‐sterile batch and fed‐batch cultures produced similar final mcl‐PHA content, monomer composition, and productivity during 24–27 h cultivations when compared to sterile control cultures for initial octanoic acid concentrations ranging from 5 to 40 mM at an initial pH of 6.5. In the absence of a P. putida inoculum, growth was eventually observed in non‐sterile medium after a lag period of up to 120 h, the length of which was dependent on the octanoic acid concentration. The efficacy of prolonged non‐sterile cultivation was tested using a sequential batch reactor (SBR). After 28 days (672 h), 1.62 g L−1 of total biomass was observed, of which the mcl‐PHA content was 47%. This resulted in a PHA titer of 0.77 g L−1, and a volumetric PHA productivity of 32 mg L−1 h−1. The polymer composition remained stable throughout at 87.0 ± 2.7% C8 monomers, and short‐chain length (scl‐) monomers were not detected. This study is the first of its kind to report that purely mcl‐PHAs were produced after prolonged periods in a non‐sterile environment and demonstrate that medium chain‐length fatty acids exert a strong selective pressure toward organisms that synthesize mcl‐PHA. This suggests an opportunity for mcl‐PHA production in open continuous cultivation, which could reduce both fixed and operating costs.

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Production of medium-chain-length polyhydroxyalkanoates by activated sludge enriched under periodic feeding with nonanoic acid

Cited by 25 publications

References 32 publications

Biosynthesis of Copolyesters Consisting of 3-Hydroxyvalerate and Medium-chain-length 3-hydroxyalkanoates by the Pseudomonas aeruginosa P-5 Strain

Biosynthesis of Copolyesters Consisting of 3-Hydroxyvalerate and Medium-chain-length 3-hydroxyalkanoates by the Pseudomonas aeruginosa P-5 Strain

Production of medium‐chain‐length PHA in octanoate‐fed enrichments dominated by Sphaerotilus sp.

Semi‐continuous non‐sterile production of medium chain‐length polyhydroxyalkanoates from fatty acids

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