Pollen morphology of Rutaceae from Brazilian forest fragments

Mir

et al. 2018

Pollen used to track structural and functional evolution in plants as well as to investigate the problems relative to plant classification. Pollen characters including ornamentation, shape, apertural pattern, pollen symmetry, colpus length, width, and margins used to detect the similarities and dissimilarities between genera and also species of the same genus. In this study pollen features of 20 monocot species belonging to 15 genera of the Amaryllidaceae, Asparagaceae, Iridaceae, Ixioliriaceae, Liliaceae, and Xanthorrhoeaceae were studied using scanning electron microscopy (SEM) and light microscopy (LM). In this study two species that is Zephyranthes citrina and Tulbaghia violacea were reported for the first time from Pakistan. Pollen grains were visualized with LM. Non-acetolyzed and acetolyzed pollen were examined using SEM. A taxonomic key was developed to highlight the variation in pollen features in order to make their systematic application for correct species identification.

Section: Methodsmentioning

confidence: 99%

Identification of monocot flora using pollen features through scanning electron microscopy

Bahadur

Mir

et al. 2018

“…The pollen shape is based on the ratio between equatorial and polar diameter, the shape, and size of pollens were determined using the method of (Punt, Hoen, Blackmore, Nilsson, & Le Thomas, 2007). For data analysis, the SPSS software was used to find out the minimum and maximum values, means (x), standard error (s), and standard deviation (SX), Confidence of interval (CI) and Coefficient of variation (CV) followed the method of (Dutra & Gasparino, 2017) by using (SPSS 16.0) software (Table 2) 2.3 | P/E index P/E ratio were determined based on the polar to equatorial diameter of the pollen by using the formula of Bahadur et al (2018), where P = polar diameter; E = equatorial diameter.…”

Section: Pollen Analysis Using Lmmentioning

confidence: 99%

Palyno‐anatomical studies of monocot taxa and its taxonomic implications using light and scanning electron microscopy

Bahadur

Zafar

et al. 2018

Palyno‐anatomical study of monocots taxa using Light and Scanning Electron Microscopy (SEM) was first time conducted with a view to evaluating their taxonomic significance. Studied plants were collected from different eco‐climatic zones of Pakistan ranges from tropical, sub‐tropical, and moist habitats. The aim of this study is to use palyno‐anatomical features for the correct identification, systematic comparison, and investigation to elucidate the taxonomic significance of these features, which are useful to taxonomists for identifying monocot taxa. A signification variation was observed in quantitative and qualitative characters by using the standard protocol of light microscopy (LM) and SEM. Epidermal cell length varied from maximum in Allium griffthianum (480 ± 35.9) μm at the adaxial surface to minimum in Canna indica (33.6 ± 8.53) μm on abaxial surface. Maximum exine thickness was observed in Canna indica (4.46) μm and minimum in Allium grifthianum (0.8) μm. Variation was observed in shape and exine ornamentation of the pollen, shape of the epidermal cell, number, size, and type of stomata, guard cell shape, and anticlinal wall pattern. Based on these palyno‐anatomical features a taxonomic key was developed, which help in the discrimination of studied taxa. In conclusion, LM and SEM pollen and epidermal morphology is explanatory, significant, and can be of special interest for the plant taxonomist in the correct identification of monocots taxa.

“…Moreover, we verified that UPGMA and PCA based on spore qualitative and quantitative morphological traits can help in the discrimination of species and genera in Thelypteridaceae. A similar analysis has been carried out with the pollen morphology of other vascular plant genera and families (Dutra & Gasparino, ; Marinho, Bove, Mendonça, & Gonçalves Lourenço Esteves, ).…”

Section: Discussionmentioning

confidence: 93%

Taxonomic importance of spore morphology in Thelypteridaceae from Northern Pakistan

Shah

Zafar

et al. 2019

Spore morphology of Thelypteridaceae species growing in Malakand Division, Northern Pakistan, was studied using both light microscopy and scanning electron microscopy. The taxa are Christella dentata and Glaphyropteridopsis erubescens in the subfamily Thelypteridoideae, and Phegopteris connectilis, Pseudophegopteris pyrrhorhachis, and Pseudophegopteris levingei in the subfamily Phegopteridoideae. The studied species exhibit differences in spore size, exospore thickness, color, and ornamentation. Spores of the studied species are monolete and medium‐sized, and shape is ellipsoidal in both polar and equatorial views. The average measurement of the polar diameter ranges from 27 μm to 31 μm, whereas in the equatorial direction it varied from 20 μm to 40 μm. The exospore thickness ranges from 1.2 μm to 2.4 μm. Reticulate, laevigate with microgranules, cristate, and coarsely echinate surface ornamentation are observed among the species. Multivariate analysis including unweighted pair group method with arithmetic mean and principal component analysis was used for the grouping and discrimination of species and genera.