Identification of monocot flora using pollen features through scanning electron microscopy

Zafar

et al. 2019

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Foliar epidermal features were based on the micromorphology of trichomes types, epidermal cells and stomatal complex. Even though each feature has its own limited taxonomic value but collectively these characteristics may be systematically important especially for the discrimination and identification of complex and problematic taxa. The systematics significance of nonglandular (NGTs) and glandular trichomes (GTs), stomatal complex and epidermal cells of Lamiaceous flora were analyzed by using the light microscopy (LM) and scanning electron microscopy (SEM). Variations on the observed epidermal appendages were divided into two basic types: glandular and nonglandular. GTs can be divided into subtypes: sessile capitate, subsessile capitate, and barrel and sunken. NGTs were also divided into subtypes: dendritic, stellate, conical, falcate, simple and 1–6 cells long having granulate and smooth surface ornamentation. NGTs were the most dominant features of both adaxial and abaxial surfaces of all observed taxa. Vitex negundo, Isodon rugosus, Colebrookea oppositifolia, and Marrubium vulgare could be demarked because of their twisted like appearance of NGTs at the abaxial surface. The Lamiaceae had both hypostomatic and amphistomatic leaf. Stomata were observed as diacytic, anisocytic, and anomocytic. Epidermal cells were found to be irregular, isodiametric, and rectangular. Based on these characters a taxonomic key was developed to delimit the closely related taxa. Distribution and morphology of the foliar epidermal trichomes through SEM highlight an important taxonomic tool used by the taxonomists as an aid to the correct identification of problematic Lamiaceae taxa.

Section: Discussionmentioning

confidence: 90%

Foliar epidermal anatomy of Lamiaceae with special emphasis on their trichomes diversity using scanning electron microscopy

Gul

Zafar

et al. 2019

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“…Photographs of pollen taken using SEM (Model JEOL JSM‐5910). The terminologies of Khalik Abdel et al () and Bahadur et al () were adopted for the ornamentation of exine of pollen.…”

Section: Methodsmentioning

confidence: 99%

Microscopic investigation of pollen morphology of Brassicaceae from Central Punjab‐Pakistan

Amina

Bhatti

et al. 2020

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The intention of the current study is to provide an account on the palynological features of Brassicaceae from Central Punjab‐Pakistan as a basis for future studies. Different morpho‐palynological characteristics both qualitative and quantitative were analyzed during this research which includes shape of pollen, diameter of pollen, P/E ratio, exine sculpturing, thickness of exine, type of pollen, shape and size of lumens, and thickness of murus. Taxonomic keys were also constructed based on pollen morphological characters for correct identification of species. This study aims to provide detailed information of pollen diversity and their exine structure based on both qualitative and quantitative characters by using Light microscopy and Scanning electron microscopy. Shape of pollen is mostly prolate, but some species also have sub‐prolate to spheroidal prolate types. Exine ornamentation in most species was reticulate, whereas micro reticulate (one species) and coarsely reticulate (one species) exine also observed in some pollen. All the pollen mentioned in this study have tricolpate apertures. Variation found in thickness of exine and other characters proved to be helpful at generic and specific level. The results reinforced the significance of pollen morphological features of family Brassicaceae and aid for valuable taxonomic tool in plant systematics.

“…The SEM has proven invaluable for observations of the surface morphology of plant materials, primarily due to the improved depth of field and high resolution, which are unobtainable with the light microscope. This tool along with light microscope (LM) allows the use of more micro‐morphological data in taxonomic and morphogenetic studies of many plant families (Ahmad et al, ; Ashfaq et al, ; Bahadur et al, ; Esfandani‐Bozchaloyi & Zaman, ; Rashid, Zafar, Ahmad, Khan, et al, ; Rashid, Zafar, Ahmad, Malik, et al, ; Sufyan et al, ; Ullah, Zafar, Ahmad, Dilbar, et al, ; Ullah, Zafar, Ahmad, Shah, et al, ; Ullah, Zafar, Amhad, Sultana, et al, ). Nowadays, SEM is widely used to analyze leaf epidermal characteristics for taxonomic studies in many fern species (Bondada, Tu, & Ma, ; Chuang & Liu, ; Lee, ; Sen & Mukhopadhay, ).…”

Section: Introductionmentioning

confidence: 99%

Leaf epidermal micromorphology and its implications in systematics of certain taxa of the fern family Pteridaceae from Northern Pakistan

Shah

Çelik

et al. 2018

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The present study is insights into foliar epidermal anatomy for characterizing clades, and their utility in taxonomic segregation of certain species of Pteridaceae from Northern Pakistan. The leaf epidermal anatomy of 10 species of Pteridaceae representing four genera were examined using light and scanning electron microscope. A micromorphological matrix was constructed for eight qualitative and 12 quantitative characters. unweighted pair group method with arithmetic means and principal components analysis statistical analysis were performed to test the validity of foliar epidermal anatomical features as method of separating species and genera, and phylogenetic clusters among species are constructed using qualitative and quantitative traits. The qualitative characters described here are shape of epidermal cells, stomata, guard cell and subsidiary cells, anticlinal wall pattern, and trichomes types which is helpful in defining groups within Pteridaceae. In addition, the size of stomata, guard cells, subsidiary cells, stomatal pore epidermal cells, and trichomes are quantitatively analyzed. All species have hypostomatic leaves. Two types of stomata were observed in studied species, anomocytic and polocytic. Anomocytic stomata were observed in three genera namely: Adiantum, Onychium, and Chielanthes whereas Pteris can be discriminated from other genera by its polocytic stomata. On the basis of multivariate analysis present study does provides sufficient information on the taxonomic importance of foliar anatomy which validate its efficacy in species and genera discrimination. From result obtained here it is further possible to use leaf micromorphologic data in ferns phylogeny and providing basis for future taxonomic delimitation in other taxa.