Microscopic investigation of pollen morphology of Brassicaceae from Central Punjab‐Pakistan

Abstract

The intention of the current study is to provide an account on the palynological features of Brassicaceae from Central Punjab‐Pakistan as a basis for future studies. Different morpho‐palynological characteristics both qualitative and quantitative were analyzed during this research which includes shape of pollen, diameter of pollen, P/E ratio, exine sculpturing, thickness of exine, type of pollen, shape and size of lumens, and thickness of murus. Taxonomic keys were also constructed based on pollen morphological characters for correct identification of species. This study aims to provide detailed information of pollen diversity and their exine structure based on both qualitative and quantitative characters by using Light microscopy and Scanning electron microscopy. Shape of pollen is mostly prolate, but some species also have sub‐prolate to spheroidal prolate types. Exine ornamentation in most species was reticulate, whereas micro reticulate (one species) and coarsely reticulate (one species) exine also observed in some pollen. All the pollen mentioned in this study have tricolpate apertures. Variation found in thickness of exine and other characters proved to be helpful at generic and specific level. The results reinforced the significance of pollen morphological features of family Brassicaceae and aid for valuable taxonomic tool in plant systematics.

Quality assurance and authentication of herbal drug (Argyrolobium roseum and Viola stocksii) through comparative light and scanning electron microscopy

“…In such situation, pollen characterization seems to be very powerful trait for discrimination of species. Scanning electron microscopy have been used as a tool to study the micromorphology of plant materials, that is, foliar epidermal anatomy (Ashfaq et al, 2019b; Bahadur et al, 2019, 2020; Gul et al, 2019a, 2019b) and pollen micromorphology (Amina et al, 2020; Ashfaq et al, 2018; Bahadur et al, 2018; Gul et al, 2019a, 2019b; Naz et al, 2019; Sufyan et al, 2018).…”

Morpho‐palynological assessment of some species of family Asteraceae and Lamiaceae of District Bannu, Pakistan on the bases of light microscope & scanning electron microscopy

“…The pollen morphology of medicinal plants was first carried out in Pakistan (Perveen & Qaiser, 1999). Amina et al (2020); Sardar, Perveen, and Khan (2013), investigate various aspects of plants such as morphology, palynology, and medicinal properties. Pollen morphology has provided an approach to the systematic relationships among the genera of Asteraceae and Lamiaceae.…”

Systematics of medicinally important weeds of genus Convolvulus: Convolvulaceae

“…Palynological characteristics are very useful. Some previous studies also show the use of scanning electron microscope for the identification of different groups of plants (Ahmad et al, 2018;Ahmed et al, 2019;Ashfaq et al, 2018;Ashfaq et al, 2019b;Amina et al, 2020;Bahadur et al, 2018b;Bahadur et al, 2019a;Bahadur et al, 2019b;Gul et al, 2019a;Gul et al, 2019b;Gul et al, 2019c;Sufyan et al, 2018;Bano et al, 2019;Qureshi et al, 2019;Arshad et al, 2019;Rashid et al, 2018).…”