Plasma level of miR‐99b may serve as potential diagnostic and short‐term prognostic markers in patients with acute cerebral infarction

Abstract

Objective The aim of the present study is to explore the potential diagnostic and prognostic value of plasma levels of miR‐99 family for patients with acute cerebral infarction (ACI). Methods A total of 112 patients who have been diagnosed with ACI were enrolled in this study, and 112 healthy volunteers were served as the controls. The plasma of the patients and controls were collected, and total RNAs were isolated, and the expression levels of miR‐99a, miR‐99b, and miR‐100 in the plasma of patients and controls were compared determined by RT‐qPCR methods; moreover, the receiver operating characteristic (ROC) curve has been drawn to determine whether the plasma levels of miR‐99b can distinguish patients with ACI from the controls; furthermore, the short‐term prognosis of the patients was evaluated by glasgow outcome scale (GOS), and the correlation between the plasma levels of miR‐99b and the GOS of the patients was evaluated. Finally, the correlation between the plasma level of miR‐99 and VEGF of ACI patients was analyzed. Results It was observed that miR‐99b was significantly decreased in the plasma of ACI patients compared with the healthy controls (P < .01), while the plasma levels of miR‐99a and miR‐100 showed no significant differences between the patients with ACI and the healthy controls; moreover, the area under the curve (AUC) of miR‐99b for the diagnosis of ACI was 0.8882 (95% confidence interval (CI), 0.8451‐0.9313), suggesting that plasma level of miR‐99b is a sensitive marker to distinguish patients with ACI from the healthy volunteers; furthermore, the serum level of miR‐99b was negatively correlated with GOS score of the patients (r = −.56, P < .001); finally, the plasma level of miR‐99b was negatively correlated with the levels of VEGF (r = −.3013, P = .0012). Conclusion miR‐99b was down‐regulated in plasma of patients with ACI, and plasma level of miR‐99b may be a potential diagnostic and prognostic marker for the diagnosis and treatment of ACI.

Acupuncture-Moxibustion Combined with Rehabilitation Training Is Conducive to Improving the Curative Effect, Cognitive Function, and Daily Activities of Patients with Cerebral Infarction

“…Patients with CI will suffer varying degrees of damage to their cognitive function (CF) and activities of daily living (ADL) due to the development of the disease, which will impose heavy economic and psychological burden to patients and their families [ 5 ]. Previous studies have shown that CI can lead to severe sequelae and even disability and death if optimal clinical decisions are not made promptly [ 6 ]. Therefore, designing a more effective and reliable treatment scheme for CI is of great significance for reducing the high mortality, disability, and recurrence rates of the disease.…”

miR-124 Is Downregulated in Serum of Acute Cerebral Infarct Patients and Shows Diagnostic and Prognostic Value

“…1 Without rapid clinical decision-making, ACI may result in severe outcomes, such as disability or even death; consequently, early diagnosis of ACI is crucial for the improvement of curative effects and the reduction of potential sequelae and mortality. 2 Currently, ACI diagnosis mainly relies on neuroimaging techniques, such as magnetic resonance imaging (MRI) and computed tomography (CT), nevertheless, the availability of this expensive device is restricted and image interpretation may be inconsistent in the early stage of stroke, which limits the efficiency of decision-making in ACI. 3 The lack of rapid and effective clinical decision-making in ACI could result in serious consequences.…”

“…6 Accumulating studies have unveiled the aberrant expression of miRNAs in the brain tissues and peripheral blood of ACI patients, implying that miRNAs are implicated in the pathogenesis, diagnosis, and prognosis of ACI. 2,7,8 miR-124 is identified as one of the most abundant miRNAs in the human brain that modulates variant biological functions of the central nervous system (CNS), and dysregulated miR-124 is tightly associated with stroke. 9 The elevated miR-124 expression has the property to reduce the infarct size and facilitate the recovery of neurological functions post-ischemic stroke.…”

Plasma miR‐409‐3p promotes acute cerebral infarction via suppressing CTRP3

“…21 Increasing evidence suggests that circulating microRNAs may be potential biomarkers for the diagnosis of ACI. [21][22][23] For instance, miR-124 levels are shown to be reduced in ACI patients and the dynamic changes of miR-124 may be useful for the monitor of ischemic stroke. 24 In the present study, we found that the expression of plasma miR-409-3p in the ACI group was higher than that in healthy controls.…”

“…ACI is a major cause of death and an important contributor to brain‐related mortality worldwide 21 . Increasing evidence suggests that circulating microRNAs may be potential biomarkers for the diagnosis of ACI 21–23 . For instance, miR‐124 levels are shown to be reduced in ACI patients and the dynamic changes of miR‐124 may be useful for the monitor of ischemic stroke 24 …”