miR-124 Is Downregulated in Serum of Acute Cerebral Infarct Patients and Shows Diagnostic and Prognostic Value

Abstract

Acute cerebral infarct (ACI) is a severe subtype of ischemic stroke. microRNAs (miRNAs) are implicated in the pathogenesis of ACI. This study investigated the expression pattern and clinical implication of miR-124 in ACI patients. Serum samples were collected from 108 healthy people and 108 ACI patients at 24 h, 48 h, and 72 h. Serum miR-124 expression was tested using qRT-PCR. The levels of interleukin (IL)-6, IL-8, and C-reactive protein (CRP) were detected using ELISA kits. The correlations between miR-124 expression and infarct classification, infarct size, risk factors, and inflammatory factors were analyzed. The diagnostic efficacy of miR-124 in ACI was analyzed by the ROC curve. ACI patients were assigned to the miR-124 high/low expression group and the incidence of poor prognosis was compared between the two groups. miR-124 expression was poorly expressed in the serum of ACI patients. The area under the ROC curve of miR-124 in the diagnosis of ACI was 0.9527, the specificity was 91.67%, and the sensitivity was 93.52%. miR-124 expression in ACI patients was not affected by infarct classification, infarct size, low-density lipoprotein level, and homocysteine level. miR-124 expression was negatively correlated with IL-6, IL-8, and CRP in ACI patients. Low expression of miR-124 was positively correlated with the poor prognosis of ACI. miR-124 was poorly expressed in the serum of ACI patients and served as a biomarker for the diagnosis and prognosis. This study shall confer a promising novel target for the diagnosis and treatment of ACI.

Fluid biomarkers in stroke: From animal models to clinical care

“…Serum miR‐134 was significantly correlated with increased expression of CRP and IL‐6 in AIS patients, suggesting that overexpression might be related to the modulation of downstream inflammation pathways. Zhou et al 142 described a positive correlation between low expression of miR‐124 and poor prognosis of AIS and a negative correlation with IL‐6, IL‐8, and CRP. However, the prognosis was defined according to the Glasgow coma scale on 30th day, which might undermine this result.…”

“…Zhou et al 142 described a positive correlation between low expression of miR-124 and poor prognosis of AIS and a negative correlation with IL-6, IL-8, and CRP. However, the prognosis was defined according to the Glasgow coma scale on 30th day, which might undermine this result.…”

Serum microRNA miR-491-5p/miR-206 Is Correlated with Poor Outcomes/Spontaneous Hemorrhagic Transformation after Ischemic Stroke: A Case Control Study

“…However, few studies have investigated the correlation between serum miRNAs and functional outcome or spontaneous HT. Only five studies performed functional prognostic analysis of the expression of six microRNAs in ischemic stroke patients [ 14 , 15 , 16 , 17 , 18 ]. However, the sample sizes were small, among which only one study obtained a sample over 100 patients [ 18 ].…”

Circulating MicroRNAs and Extracellular Vesicle-Derived MicroRNAs as Predictors of Functional Recovery in Ischemic Stroke Patients: A Systematic Review and Meta-Analysis

“…NIHSS was used as a short-term prognostic scale within 24, 48, and 72 h after stroke onset in 43 studies, and four studies assessed the long-term prognosis of IS patients with the NIHSS score at 7 days [62,84] and 3 months [70,88]. GOS score was measured in two studies at 30 days [49,59], whereas in one study physical outcomes within 24 h were evaluated by GCS [48]. mRS and BI were used to evaluate long-term prognosis.…”

“…A total of 63 miR-NAs were associated with short-term prognosis of cognitive and motor function. A total of 5 miRNAs were associated with long-term prognosis of motor function, assessed by the GOS [49,59], mRS [84], and NIHSS scores [70,88] at 1-3 months. Moreover, cognitive recovery evaluated by the MMSA, MoCA, and HAMD within 1 year was related to four miRNAs [55,[99][100][101].…”