Plant regeneration from mature embryo of commercial Indian bread wheat (Triticum aestivum L.) cultivars

Parmar, Sanjay Singh; Sainger, Manish; Chaudhary, Darshna; Jaiwal, Pawan K.

doi:10.1007/s12298-012-0101-2

Cited by 32 publications

(31 citation statements)

References 28 publications

(48 reference statements)

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“…The time needed in somatic embryo based recovering of plantlets from start of culture to transfer to soil is 57-60 days. The study identifies possibilities and strategies for acclimatisation of in vitro regenerated wheat cultivars Cakmak and Kunduru for successful acclimatisation in agreement with previous studies by Parmar et al (2012). The regeneration and embryogenic ability of wheat plumule and radicle explants varied and showed clear implications of both cultivar and explants on plant ontogenesis.…”

Section: Discussionsupporting

confidence: 84%

Somatic Embryogenesis on Plumule and Radicle Explants Obtained from Warm Water Hydroprimed Wheat (Triticum aestivum L.) cv. Kunduru and cv. Cakmak Seeds

Kahriz¹,

Kahriz²

2017

JSM

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Section: Discussionsupporting

confidence: 84%

Somatic Embryogenesis on Plumule and Radicle Explants Obtained from Warm Water Hydroprimed Wheat (Triticum aestivum L.) cv. Kunduru and cv. Cakmak Seeds

Kahriz¹,

Kahriz²

2017

JSM

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“…Only the Type II embryogenic callus (EC) is highly regenerative (Burris et al 2009). This phenomenon was also found on other monocots, such as maize (Armstrong and Green 1985;Vasil and Vasil 1994;Welter et al 1995), rice (Rueb et al 1994), sugarcane (Guiderdoni and Demarly 1988), wheat (Parmar et al 2012), and various non-food grasses (Lu and Vasil 1981;Zhang et al 2007;Li and Qu 2011). In general, two types of calluses had been classified and described depending on color, regeneration ability and texture.…”

mentioning

confidence: 75%

Inside out: high-efficiency plant regeneration and Agrobacterium-mediated transformation of upland and lowland switchgrass cultivars

et al. 2015

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Selection of pre-embryogenic callus from a core structure from mature seed-derived callus is the key for high-efficiency plant regeneration and transformation of switchgrass different cultivars. Switchgrass (Panicum virgatum L.) has been identified as a dedicated biofuel crop. For its trait improvement through biotechnological approaches, we have developed a highly efficient plant regeneration and genetic transformation protocol for both lowland and upland cultivars. We identified and separated a pre-embryogenic "core" structure from the seed-derived callus, which often leads to development of highly regenerative type II calluses. From the type II callus, plant regeneration rate of lowland cultivars Alamo and Performer reaches 95%, and upland cultivars Blackwell and Dacotah, 50 and 76%, respectively. The type II callus was also amenable for Agrobacterium-mediated transformation. Transformation efficiency of 72.8% was achieved for lowland cultivar Alamo, and 8.0% for upland cultivar Dacotah. PCR, Southern blot and GUS staining assays were performed to verify the transgenic events. High regenerative callus lines could be established in 3 months, and transgenic plants could be obtained in 2 months after Agrobacterium infection. To our knowledge, this is the first report on successful plant regeneration and recovery of transgenic plants from upland switchgrass cultivars by Agrobacterium-mediated transformation. The method presented here could be helpful in breaking through the bottleneck of regeneration and transformation of lowland and upland switchgrass cultivars and probably other recalcitrant grass crops.

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“…Higher concentration of Dicamba does not have significant effect on shoot growth but it may have effect on regeneration. (Parmar et al, 2012) observed different media and cultivars effect on Organogenesis and Callogenesis on wheat. MS media proved best as compared to N6 medium but different cultivars showed different responses to growth regulators at different levels.…”

Section: Effect Of Media On Callus Induction and Regeneration In Wheatmentioning

confidence: 89%

Tissue Culture Technology is a Breeding Approach in Wheat: An Overview

Jabeen¹,

Khaliq²,

Togo³

et al. 2016

mpb

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Culturing of plant cells, tissues and organs in controlled and disinfected conditions which includes light intensity, humidity and temperature is known as "Plant Tissue Culture" . From the past several years, regeneration of different plants under lab conditions from different plant cells and tissues has been playing a very important role in examining plant genetics and their physiology. This technique is known to help the plants in propagation and increases their soil's agronomic performance. In this review, we focus on the impact of plant tissue culture techniques in wheat crop, such as embryo culture, mature and immature embryo culture, effect of media on callus induction and regeneration, media and genotype interaction on callus induction and regeneration, somatic embryogenesis, correlation of agronomic trait on callus induction, and regeneration and somaclonal variations.

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Plant regeneration from mature embryo of commercial Indian bread wheat (Triticum aestivum L.) cultivars

Cited by 32 publications

References 28 publications

Somatic Embryogenesis on Plumule and Radicle Explants Obtained from Warm Water Hydroprimed Wheat (Triticum aestivum L.) cv. Kunduru and cv. Cakmak Seeds

Somatic Embryogenesis on Plumule and Radicle Explants Obtained from Warm Water Hydroprimed Wheat (Triticum aestivum L.) cv. Kunduru and cv. Cakmak Seeds

Inside out: high-efficiency plant regeneration and Agrobacterium-mediated transformation of upland and lowland switchgrass cultivars

Tissue Culture Technology is a Breeding Approach in Wheat: An Overview

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