Pilot plant study on nitrogen and phosphorus removal in marine wastewater by marine sediment with sequencing batch reactor

Abstract

Effective biological treatment of marine wastewater is not well-known. Accumulation of nitrogen and phosphorus from land-based effluent is a crucial cause of red-tide in marine systems. The purpose of the study is to reduce nitrogen and phosphorus in marine wastewater with a pilot plant-scale sequencing batch reactor (SBR) system by using marine sediment as eco-friendly and effective biological materials, and elucidate which bacterial strains in sludge from marine sediment influence the performance of SBR. By applying eco-friendly high efficiency marine sludge (eco-HEMS), the treatment performance was 15 m 3 d -1 of treatment amount in 4.5 m 3 of the reactor with the average removal efficiency of 89.3% for total nitrogen and 94.9% for total phosphorus at the optimal operation condition in summer. Moreover, the average removal efficiency was 84.0% for total nitrogen and 88.3% for total phosphorus in winter although biological treatment efficiency in winter is generally lower due to bacterial lower activity. These results were revealed by the DNA barcoding analysis of 16s rRNA amplicon sequencing of samples from the sludge in winter. The comparative analysis of the bacterial community composition in sludge at the high efficiency of the system showed the predominant genera Psychromonas (significantly increased to 45.6% relative abundance), Vibrio (13.3%), Gaetbulibacter (5.7%), and Psychroserpens (4.3%) in the 4 week adaptation after adding marine sediment, suggesting that those predominant bacteria influenced the treatment performance in winter.

In-situ remediation of nitrogen and phosphorus of beverage industry by potential strains Bacillus sp. (BK1) and Aspergillus sp. (BK2)

“…Ye et al found that the phosphorus accumulating fungi Mucor circinelloides have maximum utilization of 40.1% for phosphorus from the waste streams 47 whereas Dalecka et al found the removal efficiency of phosphorus 99.9% by Trametes versicolor after 6-h of incubation period whereas 99.9% of phosphorus removed by Aspergillus luchuensis after an incubation of 24 h at an initial concentration of 2600 mg/L whereas they found that the concentration of nitrogen increases from 250 to 2300 mg/L by Trametes versicolor and 200 mg/L to 1400 mg/L by Aspergillus luchuensis correspondingly from non-sterile municipal wastewater 33 . Posadas et al found that 70 ± 8% of nitrogen and 85 ± 9% of phosphorus were removed in the algal–bacterial bioreactor while the treatment of domestic wastewater at 10 day of hydraulic retention time and also observed that no phosphorus were removed while treated with bacterial biofilm 48 whereas Kim et al found that the removal efficiency of total nitrogen (89.3%) and total phosphorus (94.9%) at the optimal condition in summer whereas 84% of total nitrogen and 88.3% of total phosphorus removed in winter at an initial concentration of 7.1 ± 0.5 mg/L of total nitrogen and 1.1 ± 0.1 mg/L of total phosphorus correspondingly in marine wastewater with a pilot plant-scale sequencing batch reactor system 49 .…”

Psychroserpens Luteus Sp. Nov., Isolated fFrom Red Algae

“…So far, there are only four recognized members of the genus, P. burtonensis, P. damuponensis (Lee et al 2013), P. jangbogonensis (Baek et al 2015), P. mesophilus (Kown et al 2006). In winter, Psychroserpens helps reduce N and P in marine wastewater, thereby reducing the harmful algal blooms in marine coastal regions (Kim et al 2020). The genus Psychroserpens comprises Gram-negative, obligately aerobic, rod-shaped, oxidase-positive, yellow-to yellowish-orange-pigmented bacteria.…”