Phytohormonal Regulation of in Vitro Formation of Wheat Androgenic Structures

Seldimirova, O.A.; D.Yu., Zaytsev; Galin, Ilshat; Круглова, Н.Н.

doi:10.18413/2409-0298-2016-2-1-3-8

Cited by 4 publications

(4 citation statements)

References 10 publications

(13 reference statements)

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“…Stress and hormones influence chromatin reorganisation, which is a consequence of reprogramming transcription, and translation profiles in cells [22] play a key role in microspore embryogenesis induction [23]. Activation of the reprogramming process should result in initiation of embryogenic program and not development of non-morphogenic calli or apoptosis of microspores [24]. Moreover, in microspore embryogenesis, stress treatment initiates a rearrangement of the cytoskeleton and re-localisation of the nucleus.…”

Section: Discussionmentioning

confidence: 99%

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Effect of Zearalenone and Hormone Regulators on Microspore Embryogenesis in Anther Culture of Wheat

Weigt

Niemann

Siatkowski

et al. 2019

Plants

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The purpose of this work was to assess the impact of zearalenone (ZEN) and selected hormone regulators on the effectiveness of microspore embryogenesis in anther culture of wheat. The plant material comprised F1 hybrids of winter and spring wheat. Six combinations of media inducing microspore proliferation and formation of embryogenic structures were investigated: two combinations of growth regulators (D - 2,4-D + dicamba, K - 2,4-D + kinetin), each with three ZEN concentrations (0 mL/L, 0.1 mL/L, 0.2 mL/L). A significant increase in microspore embryogenesis effectiveness on media with the addition of ZEN was observed both at the stages of its induction and the formation of green plants in some genotypes. In case of both combinations of growth regulators, an increased concentration of ZEN resulted in more effective induction of microspore embryogenesis. The most effective induction medium was the D medium supplemented with 0.2 mL/L ZEN. As a result of the use of zearalenone together with two combinations of growth regulators, all genotypes tested produced androgenic structures, which indicates the breakdown of genotypic recalcitrant in the analysed hybrids. In addition, green plants were obtained from 18 out of 19 tested hybrids. The addition of ZEN to the medium did not affect the number of regenerated albino plants nor the number of spontaneous genome doublings proportion.

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Section: Discussionmentioning

confidence: 99%

“…Auxinic herbicide 2,4-D (2,4-dichlorophenoxyacetic acid) is the most widely applied microspore embryogenesis inducer [24,25]. According to literature, this synthetic hormone when added to an induction medium has auxin-like effects and in addition acts as a stress factor [26].…”

Section: Discussionmentioning

confidence: 99%

Effect of Zearalenone and Hormone Regulators on Microspore Embryogenesis in Anther Culture of Wheat

Weigt

Niemann

Siatkowski

et al. 2019

Plants

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“…It is known that endogenous phytohormones are released when plants are exposed to stress. The amount and type of these hormones determine the demand of cells for exogenous hormones provided in the substrate [30]. Additionally, it is necessary to remember that winter wheat is more tolerant of low temperatures.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the Androgenic Response of Spring and Winter Wheat (Triticum aestivum L.)

Weigt

Kiel

Siatkowski

et al. 2019

Plants

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Androgenesis is potentially the most effective technique for doubled haploid production of wheat. It is not however widely used in breeding programmes due to its main limitation: the genotype dependence. Due to genetic differences between spring and winter wheat, it was assumed that both phenotypes are different in their capacity to conduct androgenesis. And so, the aim of this investigation was to verify the effectiveness of androgenesis induction and plant regeneration of spring and winter wheat genotypes while considering varying amounts of growth hormones in the induction medium. Fifteen genotypes of spring wheat and fifteen of winter wheat were used in the experiment. Six hundred anthers of each of the 30 genotypes were plated and analysed. Previous studies have allowed selection of the best medium for wheat androgenesis and a combination of growth hormones that are the most effective in stimulating microspore proliferation. Therefore, C17 induction media with two combinations of growth hormones were used: I—supplemented only by auxins (2,4-D and dicamba), and II—supplemented by auxin and cytokinin (2,4-D and kinetin). Data was recorded according to the efficiency of androgenic structure formation (ASF), green plant regeneration (GPR), and albino plant regeneration (APR). The results showed that the induction and regeneration of androgenesis in the spring wheat were more efficient than in the winter ones. The spring genotypes formed more androgenic structures and green plants on anthers plated on the medium supplemented only by auxins, in contrast to the winter genotypes which were better induced and regenerated on the medium supplemented by auxin and cytokinin. The study showed that to increase the efficiency of androgenesis, it is necessary to select appropriate factors such as concentration and type of hormones in medium composition, affecting the course of the culturing procedure according to the winter or spring phenotype of donor plants.

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“…The auxin herbicide 2,4-D is widely used as growth hormone to induce embryo-like structures (Przetakiewicz et al, 2003;Seldimirova et al, 2016). Previous studies showed that this synthetic hormone added to the induction medium behaved as a stress factor and had auxin-like effects (Fehér, 2005).…”

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In vitro anther culture as efficiently applied technique for doubled haploid production of wheat (Triticum aestivum L.)

Zuhair¹,

Lantos²,

Pauk³

2021

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In vitro anther culture is the most commonly-used technology for doubled haploid production in wheat (Triticum aestivum L.) and various cereals. The technology application allows the genetic attainment of homozygous pure lines from heterozygous breeding material in a single generation, and has become a rapid alternative to the conventional breeding methods. This technology also contributes to more accurately assess QTL × environmental interactions and has been applied in: genetic researches for marker-trait association study, genomics and as a target for transformation, genetic engineering, gene mapping, and mapping of quantitative trait loci (QTLs). This paper reviews the most important factors that limit the efficiency of winter wheat (Triticum aestivum L.) anther culture, such as genotype dependency and albinism, in addition to other affecting factors including the collection time of tillers and physiological growth environments of plants. Factors that improve the efficiency of wheat anther culture in mitigating both genotypic dependency and albinism have been included in this review, too, for example, genetic improvements by following a strategy to realize this purpose through using responsive breeding material for crossing, application of convenient stress pre-treatments (cold pre-treatments, heat shock treatment, colchicine, hormones, and chemicals) to induce androgenesis, and improvement of the composition of anther culture media and culture conditions. We have also highlighted the methods of chromosome doubling for the haploid wheat produced by anther culture androgenesis, such as spontaneous chromosome doubling or use of colchicine and anti-mitotic chemicals at an early stage of anther culture or as root immersion treatment.

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Phytohormonal Regulation of in Vitro Formation of Wheat Androgenic Structures

Cited by 4 publications

References 10 publications

Effect of Zearalenone and Hormone Regulators on Microspore Embryogenesis in Anther Culture of Wheat

Effect of Zearalenone and Hormone Regulators on Microspore Embryogenesis in Anther Culture of Wheat

Comparison of the Androgenic Response of Spring and Winter Wheat (Triticum aestivum L.)

In vitro anther culture as efficiently applied technique for doubled haploid production of wheat (Triticum aestivum L.)

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