Phytochemistry, antioxidant capacity, total phenolic content and anti-inflammatory activity of Hibiscus sabdariffa leaves

Jing, Zhen; Villani, Thomas S.; Guo, Yue; Qi, Yadong; Chin, Kit Ling; Pan, Min‐Hsiung; Ho, Chi‐Tang; Simon, James E.; Wu, Qingli

doi:10.1016/j.foodchem.2015.06.006

Cited by 176 publications

(122 citation statements)

References 29 publications

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“…In 2015, Tabe and colleagues administered Hibiscus sabdariffa (Linnaeus) juice to HIV/AIDS patients on ARV therapy and reported an increase in white blood cells compared to the control group. H. sabdariffa is a plant with high antioxidant capacity and is consumed as a leafy vegetable and herbal tea in many countries [14, 15]. This study investigated the antioxidant and anti-HIV potential of H. gordonii , a popular plant which has been commercialized as a diet suppressant to aid with weight loss [2].…”

Section: Introductionmentioning

confidence: 99%

In vitro anti-HIV and antioxidant activity of Hoodia gordonii (Apocynaceae), a commercial plant product

Kapewangolo

Knott

Shithigona

et al. 2016

BMC Complement Altern Med

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Background Hoodia gordonii products are widely commercialized for anti-obesity purposes; however, minimal research is available on the other health properties demonstrated by this popular herbal plant.Methods H. gordonii crude extracts (ethanol and ethyl acetate) were assayed for in vitro anti-HIV-1 protease (PR), reverse transcriptase (RT) and integrase activity. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and reducing power assays were used for the antioxidant analysis. In addition, qualitative and quantitative phytochemical analyses of the extracts were determined using standard methods.Results H. gordonii extract demonstrated good inhibition against HIV RT with IC50 values of 73.55 ± 0.04 and 69.81 ± 9.45 μg/mL for ethanol and ethyl acetate extracts, respectively. Both extracts also demonstrated inhibitory activity against HIV PR with IC50 values of 97.29 ± 0.01 and 63.76 ± 9.01 μg/mL for ethanol and ethyl acetate extracts. In addition, H. gordonii also showed good antioxidant activity with IC50 values of 124.6 ± 11.3 and 126.2 ± 3.15 μg/mL obtained for ethanol and ethyl acetate extracts, respectively. The reducing power of H. gordonii extracts increased as the concentration increased which confirmed the presence of antioxidants (reductants) in the extracts. Phytochemical screening of H. gordonii revealed the presence of phenolics, alkaloids, terpenes, steroids, cardiac glycosides and tannins in the ethanolic extract, while the ethyl acetate extract only showed the presence of phenolics, cardiac glycosides and steroids. The total phenolic content was 420 ± 0.17 and 319.9 ± 0.2 mg GAE/g for the ethanol and ethyl acetate extracts, respectively. The ethanol extract, which revealed the presence of tannins, had a tannin content of 330 ± 0.2 mg TAE/g extract.ConclusionThis data suggests that H. gordonii has good in vitro inhibition against selected HIV-1 enzymes as well as antioxidant properties, suggesting new potential uses for this commercial plant.

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Section: Introductionmentioning

confidence: 99%

In vitro anti-HIV and antioxidant activity of Hoodia gordonii (Apocynaceae), a commercial plant product

Kapewangolo

Knott

Shithigona

et al. 2016

BMC Complement Altern Med

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“…Substances with the potential to donate electron will convert blue-green colored ABTS radical solution into a colorless neutral form. The ABTS scavenging activity is widely used to evaluate radical scavenging potential of various plants [17,33,[41][42][43][44][45][46][47]. In the present study, we determined the antiradical activity of N. plumbaginifolia by ABTS assay, and the result is shown in Fig.…”

Section: Abts Radical Scavenging Activity Of N Plumbaginifoliamentioning

confidence: 90%

Insecticidal, Antibacterial, and Antiradical Activity of Nicotiana Plumbaginifolia Viv. (Solanaceae)

Tr¹,

Mr²,

Hc³

2017

Asian J Pharm Clin Res

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Objectives: Nicotiana plumbaginifolia Viv. belongs to the family Solanaceae. The present study was carried out to determine insecticidal, antibacterial, and antiradical activity of aerial parts of N. plumbaginifolia.Methods: Extraction was carried out by maceration process using methanol. Insecticidal activity was assessed, in terms of larvicidal effect, against II and IV instar larvae of Aedes aegypti. Antibacterial activity was evaluated against a panel of 7 bacteria by agar well diffusion assay. Antiradical activity was determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azinobis 3-ethylbenzothiazoline 6-sulfonate (ABTS) free radical scavenging assays. Results:The extract was found to cause dose-dependent mortality of larvae of A. aegypti. The lethal concentration 50 value of extract for II and IV instar larvae was found to be 0.41 and 0.99 mg/ml, respectively. Extract was effective in inhibiting all bacteria. Gram-positive bacteria displayed marked susceptibility when compared to Gram-negative bacteria. Bacillus cereus and Escherichia coli were susceptible to highest and least extent, respectively. Extract scavenged both DPPH radicals and ABTS radicals dose dependently. Extract scavenged ABTS radicals more efficiently (inhibitory concentration [IC 50 ] value 13.51 µg/ml) when compared to DPPH radicals (IC 50 value 17.43 µg/ml). Conclusions:The plant N. plumbaginifolia appears to be a promising resource for developing agents with insecticidal, antibacterial, and antiradical activity. The observed bioactivities could be attributed to the presence of phytochemicals which are to be isolated, characterized, and subjected for bioactivity determinations. The plant can be used to prevent arboviral diseases, infectious diseases, and oxidative damage.

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“…In a recent study, Zhen et al (2016) showed the inhibitory capacity of the H. sabdariffa leaf extract on the nitric oxide synthase activity by LPS stimulated murine macrophage. In the present study, the lucigenin-dependent chemiluminescence (CL) and the intracellular fluorescent probe DCFH-DA were used to evaluate the extra- and intracellular ROS production resulting mainly from NADPH oxidase activity by stimulated neutrophil and HL-60 cells (Derochette et al 2013).…”

Section: 2 Antioxidant Activitymentioning

confidence: 99%

Comparison of metabolic profiles and bioactivities of the leaves of three edible CongoleseHibiscusspecies

Kapepula

Ngombe

Tshibangu

et al. 2017

Natural Product Research

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Methanolic and dichloromethane extracts from the leaves of Congolese Hibiscus species were characterised by chromatographic and spectroscopic techniques and their in vitro biochemical activities against ROS production were evaluated in cellular models and on an enzyme, myeloperoxidase (MPO), involved in inflammation. Hibiscus acetosella has a chemical fingerprint different from Hibiscus cannabinus and Hibiscus sabdariffa both having similar fingerprints. Major compounds were polyphenols, represented mainly by caffeoyl-hydroxycitric acid for H. acetosella and neochlorogenic acid for the two other spiecies. All extracts displayed high cellular antioxidant activity with IC50 values ranging from 0.5 to 3 μg mL−1 using lucigenm on neutrophils. Dichloromethane extracts showed more efficient effects on extracellular ROS production and MPO activity. Antioxidant and anti-inflammatory activities of caffeoyl-hydroxycitric acid were significantly higher than those of neochlorogenic acid. The bioactivities of Hibiscus species were positively correlated with their phytochemical content and could justify the use as local nutraceutical resources and medicines.

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Phytochemistry, antioxidant capacity, total phenolic content and anti-inflammatory activity of Hibiscus sabdariffa leaves

Cited by 176 publications

References 29 publications

In vitro anti-HIV and antioxidant activity of Hoodia gordonii (Apocynaceae), a commercial plant product

In vitro anti-HIV and antioxidant activity of Hoodia gordonii (Apocynaceae), a commercial plant product

Insecticidal, Antibacterial, and Antiradical Activity of Nicotiana Plumbaginifolia Viv. (Solanaceae)

Comparison of metabolic profiles and bioactivities of the leaves of three edible CongoleseHibiscusspecies

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