Phytochemical and antibacterial evaluation of essential oils from Ottonia martiana miq. (Piperaceae)

Abstract

Três óleos essenciais extraídos das folhas, frutos e raízes de Ottonia martiana Miq. (Piperaceae), espécie comum da floresta Atlântica brasileira, e conhecida popularmente por "anestésia", foram analisados por CG-EM e submetidos a um ensaio antibacteriano bioautográfico frente a Staphylococcus aureus (ATCC 25923), Staphylococcus epidermidis (ATCC 12228), Pseudomonas aerogenes (ATCC 27853) e Escherichia coli (ATCC 25922). Setenta e sete compostos foram identificados e submetidos a uma análise comparativa, a qual revelou uma variabilidade no teor dos componentes majoritários desses óleos (espatulenol, óxido de cariofileno, (E)-nerolidol, viridiflorol, β-cariofileno, δ cadineno e aloaromadendreno). A presença de zonas de inibição de crescimento bacteriano nos bioautogramas analisados (Rfs 0,29 e 0,34) revelou o potencial antibacteriano dos óleos analisados frente às bactérias Gram-positivas testadas e permitiu identificar alguns dos componentes bioativos.Three essential oils extracted from leaves, fruits and roots of Ottonia martiana Miq. (Piperaceae), common species in Brazilian Rain Forest, known as "anestésia", were analyzed by GC-MS and tested in an antibacterial assay against Staphylococcus aureus (ATCC 25923), Staphylococcus epidermidis (ATCC 12228), Pseudomonas aerogenes (ATCC 27853) and Escherichia coli (ATCC 25922). Seventy-seven compounds were identified and submitted to a comparative analysis, which revealed variability on the amount of principal components of these oils (spathulenol, caryophyllene oxide, (E)-nerolidol, viridiflorol, β-caryophyllene, δ cadinene and alloaromadendrene). Inhibition zones of bacterial growth in the bioautograms (Rfs 0.29 and 0.34) showed antimicrobial activity of essential oils against tested Gram-positive bacteria and permitted to identify some bioactive components.

Exploration of Flesh Dammar (Shorea Leprosula Miq) and Cat Eye Dammar (Shorea Javanica K.Et.V.) As Antibacterial Agent

“…Those compounds were also present in Ottonia martiana which had similar antibacterial activity spectrum. Essential oil of leaves, fruits, and roots of O. martiana could inhibit the growth of S. aureus and S. epidermidis, but did not inhibit P. aeruginosa and E. coli [8]. In comparison, Vukovic et al [9] reported that trans-caryophyllene was present in essential oil from Teucrium montanum which effectively inhibited the growth of some Gram-negative bacteria (Agrobacterium tumefaciens, Azotobacter chlorococcum, Enterobacter cloaceae, Erwinia carotovora, Klebsiella pneumonia, P. glycinea, P.…”

Investigation of local anesthetic and antimycobacterial activity of Ottonia martiana Miq. (Piperaceae)

“…Isolated compounds must also be considered to determine if there is any synergistic effect of the compounds present in the crude extract. It has been reported that EE of O. martiana contains b-caryophyllene, a sesquiterpene known as a local anesthetic (Cunico et al 2007, Ghelardini et al 2001.…”

“…Seventy-seven compounds were identified as components of three essential oils extracted from leaves, fruits and roots of this plant (Cunico et al 2007). These chemical compounds were previously isolated from a number of closely related Piperaceae species (Giesbrecht et al 1981, Makapugay et al 1983, McFerren and Rodrigues 1998, Price and Pinder 1970, Pring 1982, Costa and Mors 1981.…”

“…Biological studies with the crude extracts of roots and aerial parts of O. martiana showed antimicrobial activity against both human and plant pathogens (Cunico et al 2006(Cunico et al , 2007. However, to date, there are no records of related pharmacological studies.…”

AVALIAÇÃO DO REAL POTENCIAL INIBIDOR DE EXTRATOS ETANÓLICOS DE Ottonia martiana SOBRE Cylindrocladium spathulatum E Botrytis cinerea

“…Estudos fitoquímicos resultaram no isolamento e identificação das amidas piperovatina, isopiperovatina, piperlonguminina e isopiperlonguminina (CUNICO et al, 2005). Sua ação antifúngica foi posteriormente relatada por Cunico et al (2007).…”