Phytochemical analysis, antimicrobial and radical-scavenging properties of Acalypha manniana leaves

Abstract

Acalypha manniana (Euphorbiaceae) is a plant popularly used in Cameroon and in several parts of Africa for the treatment of various microbial diseases like diarrhea and skin infections.The present study was designed to evaluate the phytochemical composition, antimicrobial and radical-scavenging activities of A. manniana methanol leaf extract and its fractions.The methanol extract was partitioned into hexane, ethyl acetate and residual fractions and phytochemical analysis was conducted using standard methods. The broth microdilution method was used to evaluate the antimicrobial activity against nine bacterial species and four dermatophyte species. The free radical scavenging activities of the methanol extract and its fractions were evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay.The results obtained showed that A. manniana contains alkaloids, tannins, anthocyanins, flavonoids, phenols and steroids. The methanol extract as well as the hexane, ethyl acetate and residual fractions exhibited both antibacterial and antidermatophytic activities that varied between the microbial species (MIC = 0.12 – 2.04 mg/mL). These tested samples also showed high radical-scavenging activities (RaS50 = 3.34 – 4.80 μg/mL) when compared with vitamin C used as reference antioxidant (RaS50 = 1.74 μg/mL).These findings provide evidence that the studied plant possesses antimicrobial and antioxidant properties and may act as potential antioxidant for biological systems susceptible to free radical-mediated reactions.

Metabolite Fingerprinting and Antioxidant Potential of Tartary Buckwheat- an Underutilized Pseudocereal crop from Kashmir Region

“…Our results are in accordance with the studies on Phodopyyllum hexandrum 36 and Acalypha manniana. 37 Metabolite profiling of tartary buckwheat leaf and groat methanolic extract was done by GC-MS and a single metabolic profile can be thought of as a snapshot of the metabolic state of an organism at a given moment. In medicinal chemistry, metabolite profiling is of paramount importance to ascertain the chemo-typing of natural products that will not only allow us to scientifically determine but validate their traditional uses, pharmacological activities and therapeutic potential.…”

Medicinal plants from the genus Acalypha (Euphorbiaceae)–A review of their ethnopharmacology and phytochemistry

“…The leaf extract showed the presence of alkaloids, phenols, flavonoids, anthraquinones, anthocyanins, tannins and steroids (Noumedem et al, 2013). ϰϯ Anticancer 9-(3,6-dimethyl-hepta-2,6-dienyl)-hypoxantine Svaþinova, 2011 ϰϰ respectively (Moussa et al, 2011).…”

“…In the western region of Cameroon, leaf decoction of the plant is used to treat mycosis and skin diseases (Noumedem et al, 2013). A leafy stem decoction is taken against diarrhea in some African countries namely Ivory Coast, Ghana, Uganga, Rwanda, Burundi and Cameroon.…”

Antioxidant, antimicrobial, and GC-MS profiling of Saussurea obvallata (Brahma Kamal) from Uttarakhand Himalaya

“…The bacterial strains included three Gram-negative strains namely, Pseudomonas aeruginosa (MTCC 4306), Escherichia coli (MTCC 1698), and Klebsiella pneumoniae (MTCC 9544) and one Gram-positive strain namely, Staphylococcus aureus (MTCC 6908). The antibacterial activity of the extracts was estimated using the well diffusion method [17,18]. Briefly, the extracts (methanolic or aqueous) were tested on Luria Bertani agar (LB agar; Himedia, Delhi, India) plates to detect antibacterial activity, if any, against P. aeruginosa, E. coli, K. pneumoniae and S. aureus.…”

“…The well diffusion method was also used to screen the antifungal activity of S. obvallata extracts against three fungal strains of Candida namely, C. albicans (MTCC 3017), C. glabrata (MTCC 3019) and C. tropicalis (MTCC 3416) according to the same protocol [17,18] giving in the previous section (antibacterial activity). These strains were also procured from the Institute of Microbial Technology (IMTECH), Chandigarh, India.…”