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Laboratory bench scaling was done and an average of 1.85 fold increase by Response Surface Methodology (RSM) optimization was obtained. It was found that the predicted value (4.96 IU/ml) obtained by RSM is in close accordance with observed activity 5.14 IU/ml. Endoglucanases are mainly induced by CMC while Wheat bran (natural substrate) exoglucanase is more active when induced by avicel and cellulose. Addition of substrate beyond a level caused inhibition of cellulase production. The molecular weight of protein as determined by SDS-PAGE is very similar to molecular weight of cellulase of Trichoderma viride (T. viride) cellulase and Trichoderma reesei (T. reesei) endoglucanase. T. reesei β-glucosidase has high enzymatic activity on CMC substrate when compared with T. viride β-glucosidase. Secondary structure analysed by using Circular Dichroism confirmed that composition of celluase system is very similar to other analysed species. The cellulase was found to be active in pH range of 4.8-5.5; while temperature range varied from 50°C to 70°C. Although the enzymatic activity produced by mutants were lesser than the parent, but in one case mutants of Trichoderma reesei’s BGL has shown higher activity on cellulose.

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iZVleČeK Živorodnost pri ajdi (Fagopyrum esculentum)Navadna ajda (Fagopyrum esculentum Moench) je gospodarsko pomembna poljščina, pri kateri je opazen edinstven pojav živorodnosti (viviparija), ki je običajno lastnost rastlin, ki rastejo na mokrih, suhih in poplavnih območjih. Živorodnost so opazili pri zrelih in nezrelih (zelenih) semenih na socvetju. Izražanje te lastnosti pri ajdi zmanjša uporabno vrednost pridelka.Ključne besede: Fagopyrum, ajda, živorodnost, kalitev abStRact Vivipary in Fagopyrum esculentum Common buckwheat (Fagopyrum esculentum Moench) is an economically important crop, exhibiting a unique phenomenon of vivipary, which is usually a trait shown by plants growing in wet, arid and flodded conditions. Vivipary was observed in both ripe and un-ripe (green) seeds on the inflorescence. The expression of this trait in buckwheat is detrimental for the crop yield.

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