Physiological and ultrastructural responses during drying of recalcitrant seeds of <i>Araucaria angustifolia</i>

Gasparin, Ezequiel; Faria, José Márcio Rocha; José, Anderson Cleiton; Hilhorst, H.W.M.

doi:10.15258/sst.2017.45.1.01

Cited by 8 publications

(8 citation statements)

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“…Among the groups that persist in the area, the panel that was demonstrated up to two decades ago did not change much afterward (Figure 1). The majority of the studies are basically focused on desiccation tolerance Corsato et al, 2012;Chapell et al, 2015;Amoedo and Ferraz, 2017;Gasparin et al, 2017;Subbiah et al, 2017;Chandra and Keshavkant, 2018;Plitta-Michalak et al, 2018), identification of storage behavior (Comin et al, 2014;Joshi et al, 2015;Vicente et al, 2016; 2017; Plitta-Michalak et al, 2018), analysis of deterioration and evaluation of techniques to extend the storage period (Barbedo and Cicero, 2000;Andréo et al, 2006;Bonjovani and Barbedo, 2008;Ballesteros et al, 2014;Lan et al, 2014;Parkhey et al, 2014;Garcia et al, 2015;Ming-Yue et al, 2015;Umarani et al, 2015;Araldi et al, 2016;Hossel et al, 2016;Félix et al, 2017), and rare studying other aspects such as seed maturation (Avila et al, 2009;Lamarca et al, 2013b;Mata et al, 2013;Schultz et al, 2014;Araldi and Coelho, 2015;Shibata and Coelho, 2016;Souza et al, 2018a, b), whose importance will be discussed later, and ecological (Ramlall et al, 2015;Vaz et al, 2016;Porto et al, 2018) or germinative aspects (Moothoo-Padayachie et al, 2016). An analysis, albeit superficial, of studies developed in Brazil and in the world shows small differences.…”

Section: The Research With Recalcitrant Seedsmentioning

confidence: 99%

A new approach towards the so-called recalcitrant seeds

Barbedo

2018

J. Seed Sci.

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Water is essential, irreplaceable, and indispensable for any kind of carbon-based-life metabolic activity. Water-dependent living beings are the expected pattern in nature. However, some organisms can survive for some time at a minimum water content, such as seeds of some species (orthodox seeds). Nevertheless, the expected standard life behavior is found in seeds of another group of species, the so-called recalcitrant seeds, which are sensitive to desiccation. A huge range of different behaviors can be found between these two groups, leading authors to consider that orthodoxy and recalcitrance is not an all-or-nothing situation. Notwithstanding, we are still too far from understanding the differences and similarities between all these kinds of seeds and this has been a serious barrier to the development of plant conservation technologies. A new approach to understanding the differences between these seeds is presented here based on seed maturation, environmental influences, and evolution. From this point of view, all kinds of seed behavior are contemplated and, consequently, some new perspectives are considered for the recalcitrant seed conservation technology, the most intensely desired technology nowadays in this area.

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Section: The Research With Recalcitrant Seedsmentioning

confidence: 99%

A new approach towards the so-called recalcitrant seeds

Barbedo

2018

J. Seed Sci.

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“…These cells comprised part of the percentage of cells in the G2 phase as observed by flow cytometry (8 to 10%). There have been no previous reports regarding DNA content during A. angustifolia seed development; however, freshly harvested and germinated mature seeds have been found not to differ in 2C DNA content (Gasparin et al, 2017). These observations demonstrate that the seeds of A. angustifolia, even in the process of germination, seem to modify their DNA content late, probably after protrusion of the radicle.…”

Section: Discussionmentioning

confidence: 54%

“…The maturation period of A. angustifolia seeds is between April and August (Mattos, 2011), which are classified as recalcitrant (Gasparin, Faria, José, & Hilhorst, 2017). In previous studies during the maturation period, morphological and physiological alterations in seeds were observed (Shibata, Coelho, & Steiner, 2013).…”

Section: Introductionmentioning

confidence: 99%

Anatomical and biochemical changes associated with the development and germination of <i>Araucaria angustifolia</i> seeds

Shibata

Coelho

Schmidt

et al. 2019

Acta Sci. Biol. Sci.

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This study aimed at assessing the cell cycle, and anatomical and biochemical changes that the embryonic axis of Araucaria angustifolia undergoes during development, focusing on the maturation stage. During all development, cells exhibited intense metabolic activity with an abundance of mitochondria, lipid bodies, and vacuolated cells. The continued accumulation of starch and protein was observed by LM and TEM and indicated by spectra of FTIR. Cell differentiation of the procambium was observed with a thickening of the cell wall and the formation of resiniferous ducts. At Stage III and IV, cells exhibited structural changes such as altered or elongated mitochondria and presence of plastoglobules. These results suggest that there is a gradual transition from developmental metabolism to germination metabolism. Such changes can contribute to the rapid germination of seeds right after their dispersion, making it an ecological strategy to reduce post-dispersal exposure to predators and to avoid damage from reduced moisture.

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“…In Brazil, the haphazard exploitation of the Ombrophilous Mixed Forest (Araucaria Forest) resulted in a drastic reduction in Araucaria angustifolia population, a species which dominates this type of forest (Maran et al, 2016;Pelissari et al, 2016;Gasparin et al, 2017). A. angustifolia (commonly called araucaria) is the only species of Araucaria genus that occurs naturally in Brazil and it has significant economic and ecological values.…”

Section: Introductionmentioning

confidence: 99%

“…Araucaria seeds are recalcitrant and therefore difficult to preserve (Gasparin et al, 2017;Zanette et al, 2017). Despite its quick loss of viability after desiccation, they remain metabolically active (Berjak & Pammenter, 2013;Pammenter & Berjak, 2014).…”

Section: Introductionmentioning

confidence: 99%

Viability of embryonic axes of Araucaria angustifolia after freezing using two cryopreservation methods

Frizzo

Quoirin

2018

Pesq. flor. bras.

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Araucaria angustifolia (Bertol.) O. Kuntze é uma das espécies nativas do Brasil com maior importância na Região Sul. No entanto, suas sementes são recalcitrantes, constituindo um obstáculo para a sua conservação em longo prazo, sendo a criopreservação uma alternativa viável para o armazenamento de seu germoplasma. Os eixos embrionários (EE) excisados das sementes de araucária foram encapsulados, desidratados e submetidos a dois métodos de criopreservação: o resfriamento rápido, congelando em nitrogênio líquido (NL) durante 2 h, e o pré-resfriamento a - 40 °C, seguido do congelamento em nitrogênio líquido durante 2 h. Posteriormente, os EE foram descongelados rapidamente e avaliados quanto à integridade do DNA pelo teste bioquímico do tetrazólio, germinação in vitro e ocorrência de oxidação. Tanto o DNA dos EE que não foram criopreservados quanto o daqueles que foram criopreservados mantiveram sua integridade. Os resultados do teste de tetrazólio indicaram que a maioria dos EE desidratados pelo congelamento rápido permaneceu viável. Após 15 dias de cultivo in vitro, os EE não germinaram e apresentaram sinais de oxidação. Os métodos de encapsulamento-desidratação seguidos de congelamento rápido são promissores para a criopreservação de EE de araucária, como demonstrado pelo teste de tetrazólio e da manutenção da integridade total do DNA.

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Physiological and ultrastructural responses during drying of recalcitrant seeds of Araucaria angustifolia

Cited by 8 publications

References 0 publications

A new approach towards the so-called recalcitrant seeds

A new approach towards the so-called recalcitrant seeds

Anatomical and biochemical changes associated with the development and germination of <i>Araucaria angustifolia</i> seeds

Viability of embryonic axes of Araucaria angustifolia after freezing using two cryopreservation methods

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