Physicochemical characterization of albumin immobilized on different TiO2 surfaces for use in implant materials

Bronze‐Uhle, Erika S.; Dias, Leonardo Francisco Gonçalves; Trino, Luciana D.; Matos, Adriana Arruda; Oliveira, Rodrigo Cardoso de; Lisboa‐Filho, Paulo Noronha

doi:10.1016/j.colsurfa.2018.12.028

Cited by 23 publications

(6 citation statements)

References 91 publications

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“…HSA is in general a soft protein. Experimentally it was found that after immobilization or adsorption on TiO 2 surfaces [49] this soft blood protein undergoes conformational changes induced by the surface topography affecting also its tertiary structure, then the functionality [50][51][52][53][54][55][56][57][58]. These studies confirm the importance not only of the similar surface chemistry of TiO 2 surfaces but also the influence of the surface curvature on the protein-biomaterial interaction process.…”

Section: Introductionmentioning

confidence: 68%

Surface Chemistry, Crystal Structure, Size and Topography Role in the Albumin Adsorption Process on TiO2 Anatase Crystallographic Faces and Its 3D-Nanocrystal: A Molecular Dynamics Study

Raffaini

2021

Coatings

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TiO2 is widely used in biomaterial implants. The topography, chemical and structural properties of titania surfaces are an important aspect to study. The size of TiO2 nanoparticles synthetized by sol–gel method can influence the responses in the biological environment, and by using appropriate heat treatments different contents of different polymorphs can be formed. Protein adsorption is a crucial step for the biological responses, involving, in particular, albumin, the most abundant blood protein. In this theoretical work, using molecular mechanics and molecular dynamics methods, the adsorption process of an albumin subdomain is reported both onto specific different crystallographic faces of TiO2 anatase and also on its ideal three-dimensional nanosized crystal, using the simulation protocol proposed in my previous theoretical studies about the adsorption process on hydrophobic ordered graphene-like or hydrophilic amorphous polymeric surfaces. The different surface chemistry of anatase crystalline faces and the nanocrystal topography influence the adsorption process, in particular the interaction strength and protein fragment conformation, then its biological activity. This theoretical study can be a useful tool to better understand how the surface chemistry, crystal structure, size and topography play a key role in protein adsorption process onto anatase surface so widely used as biomaterial.

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Section: Introductionmentioning

confidence: 68%

Surface Chemistry, Crystal Structure, Size and Topography Role in the Albumin Adsorption Process on TiO2 Anatase Crystallographic Faces and Its 3D-Nanocrystal: A Molecular Dynamics Study

Raffaini

2021

Coatings

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“…The reason for the low hemolysis rate of the sample might be as follows: For Ti substrate, it had a flat surface and did not exert mechanical force on the red blood cells. For TiO 2 /Ti, it had good hydrophilicity and could preferentially adsorb albumin in blood after the construction of the TiO 2 nanorod array on the surface of substrate. , The albumin on the material surface could serve as a shielding layer for red blood cells to adhere to the material surface. Therefore, the contact of red blood cells with the material was reduced, thereby effectively preventing the rupture of red blood cells.…”

Section: Results and Discussionmentioning

confidence: 99%

Fabrication of a TiO₂@Cu Core–Shell Nanorod Array as Coating for Titanium Substrate with Mechanical and Chemical Dual Antibacterial Property

Zhang

Chen

Lin

et al. 2022

ACS Appl. Bio Mater.

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Titanium (Ti) is an excellent medical metal material, but the absence of good antibacterial property restricts its widespread application. To overcome this, we thus conducted a series of modifications for Ti. First, a titanium dioxide (TiO2) nanorod array was generated on the Ti surface by hydrothermal treatment (TiO2/Ti). With the polymer-mediated self-assembly method, a continuous copper (Cu) shell structure on the surface of the nanorod was then generated to form a TiO2@Cu core–shell nanorod array as coating for Ti (TiO2@Cu/Ti). Using pure Ti as the control group, the antibacterial properties of TiO2/Ti and TiO2@Cu/Ti were appraised. The results manifested that the mechanical and chemical dual function of the released Cu2+ and TiO2 nanorod array could effectively kill bacteria on the surface of Ti. Besides, the obtained coating exhibited no cytotoxicity and favorable biocompatibility. In this work, we found an antibacterial strategy based on multiple sterilization pathways, which made Ti have good antibacterial property and further improved its biocompatibility.

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“…Particularly, titania coatings are widely applied for improving the biocompatibility and bioactivity of implant materials or for the biosensing purposes, where a wide range of biomolecules can be immobilized to its surface and provide specific sensing functions [8]. In fact, the excellent biocompatibility of titanium itself is owed to a thin, several nm thick amorphous TiO 2 film naturally formed on its surface, protecting titania from various external environments, which could deteriorate the properties of the resulting surface [9].…”

Section: Introductionmentioning

confidence: 99%

Effect of Various Surface Treatments on Wettability and Morphological Properties of Titanium Oxide Thin Films

et al. 2022

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The effect of three popular surface activation methods for a titanium oxide (titania) surface was thoroughly investigated to identify the most effective protocol for the enhancement of hydrophilicity. All the methods, namely H2O2 activation, UV irradiation and oxygen plasma treatment resulted in an enhanced hydrophilic titania surface, which was evidenced by the reduced contact angle values. To study in detail the chemical and morphological features responsible for the increased hydrophilicity, the treated surfaces were submitted to inspection with atomic force microscopy and X-ray photoelectron spectroscopy. The correlation between the treatment and titania surface hydroxylation as well as hydrophilic behavior have been discussed.

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Physicochemical characterization of albumin immobilized on different TiO2 surfaces for use in implant materials

Cited by 23 publications

References 91 publications

Surface Chemistry, Crystal Structure, Size and Topography Role in the Albumin Adsorption Process on TiO2 Anatase Crystallographic Faces and Its 3D-Nanocrystal: A Molecular Dynamics Study

Surface Chemistry, Crystal Structure, Size and Topography Role in the Albumin Adsorption Process on TiO2 Anatase Crystallographic Faces and Its 3D-Nanocrystal: A Molecular Dynamics Study

Fabrication of a TiO₂@Cu Core–Shell Nanorod Array as Coating for Titanium Substrate with Mechanical and Chemical Dual Antibacterial Property

Effect of Various Surface Treatments on Wettability and Morphological Properties of Titanium Oxide Thin Films

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Physicochemical characterization of albumin immobilized on different TiO2 surfaces for use in implant materials

Cited by 23 publications

References 91 publications

Surface Chemistry, Crystal Structure, Size and Topography Role in the Albumin Adsorption Process on TiO2 Anatase Crystallographic Faces and Its 3D-Nanocrystal: A Molecular Dynamics Study

Surface Chemistry, Crystal Structure, Size and Topography Role in the Albumin Adsorption Process on TiO2 Anatase Crystallographic Faces and Its 3D-Nanocrystal: A Molecular Dynamics Study

Fabrication of a TiO2@Cu Core–Shell Nanorod Array as Coating for Titanium Substrate with Mechanical and Chemical Dual Antibacterial Property

Effect of Various Surface Treatments on Wettability and Morphological Properties of Titanium Oxide Thin Films

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Fabrication of a TiO₂@Cu Core–Shell Nanorod Array as Coating for Titanium Substrate with Mechanical and Chemical Dual Antibacterial Property