Physico-chemical characterization and biological response of Labeo rohita-derived hydroxyapatite scaffold

Abstract

The chemically treated Labeo rohita scale is used for synthesizing hydroxyapatite (HAp) biomaterials. Thermogravimetric and differential thermal analyses of fish scale materials reveal the different phase changes with temperature and find out the suitable calcination temperatures. The composition and structures of wet ball-milled calcined HAp powders are characterized by Fourier transform infrared spectroscopy, X-ray diffraction, field emission scanning electron microscopy, transmission electron microscopy, energy dispersive X-ray analysis (EDX). The EDX as well as chemical analysis of fish scale-derived apatite materials confirms that the Ca/P ratio is 1.71. The compressive stress, hardness and porosity have been evaluated on sintered HAp biomaterials. The cell attachment on HAp surfaces, cytotoxicity evaluation and MTT assay, which are carried out in RAW macrophage-like cell line media demonstrate good biocompatibility. The histological analysis also supports the bioaffinity of processed HAp biomaterials in Wistar rat model for investigating the contact reaction and stability at the artificial or natural prosthesis interface.

Production, characterization, and cytotoxicity of calcium phosphate ceramics derived from the bone of meagre fish, Argyrosomus regius

“…The total mass loss at this temperature range was about 59% for the fish bone. Thermal analysis of the sample exhibited a three major weight change stage and similar observations have been previously reported in the literature [38,42,43]. At the first stage, an initial weight loss of approximately < 3% was observed corresponding to the temperature range of 20-200°C and that was attributed to the release of the adsorbed water on the surface of the fish bones.…”

Improvement of Drug-Loading Properties of Hydroxyapatite Particles Using Triethylamine as a Capping Agent: A Novel Approach

“…In the past decade, the development of particle drug-delivery systems has been the main focus of drug-loading research. Among these systems, bioceramic particles have attracted more and more attention due to their excellent biocompatibility [1], bioaffinity [2], biological activity [3], high stability [4], and sufficient mechanical properties [5]. Hydroxyapatite is a calcium phosphate bioceramic [6] that is widely used as a bone substitute because nanohydroxyapatite particles (n-HAPs) are one of the components of natural bone [7].…”

“…Furthermore, the low density of mesoporous nanostructures allows the oral drug to float in the gastrointestinal tract, thus prolonging the gastric retention time [11]. In addition to the above advantages, HAPs have the following advantages: (1) HAPs usually have a long biodegradation time and can be retained for a long time after administration without the transient release of the drug, so that the drug release can be controlled at the lowest effective concentration for a long time, avoiding the issues of repeated administration and high-dose side effects [12]; (2) the crystal structure of a HAP is hexagonal and it has two different binding sites, namely the negatively-charged P site and the positively-charged Ca site. These characteristics make HAPs a good adsorbent that can bind to many substances, including proteins, antitoxins, and growth factors [13]; (3) by applying doping techniques, HAPs can be prepared as particles with good electrical properties (such as ferroelectric and dielectric properties) [14], mechanical properties (such as piezoelectric property, ultrahigh hardness) [15], magnetic properties (such as superparamagnetism) [16] and optical properties (such as photothermal effect, electroluminescence) [17].…”

Evaluation of physical and mechanical properties of B-tri-calcium phosphate/poly-3-hydroxybutyrate nanocomposite scaffold for bone tissue engineering application

“…According to the results of porosity measurements, it may be concluded that the samples immersed for 30 sec in P3HB had higher porosity than the samples immersed for 1 min in P3HB. Mondal et al [28] revealed that the porosity of Labeo rohita sh scale-derived sca old was about 30-33%. Kang et al [37] determined that the porosity of -TCP sca old was about 82.49% and slightly declined up to 80.65% after PLGA in ltration.…”

“…Consequently, the optimal sca old design and fabrication techniques must be able to create porous structures convenient for attaining the desired mechanical function and mass transport properties [28]. Polymer replication methods enable the sca olds formation with uniform and adjustable porosity [29][30][31].…”