Physical and bioactive properties of multi-layered PCL/silica composite scaffolds for bone tissue regeneration

Lee, Hyeongjin; Hwang, Hyun-Suk; Kim, YeSeul; Jeon, Hyerin; Kim, GeunHyung

doi:10.1016/j.cej.2014.04.009

Cited by 35 publications

(14 citation statements)

References 40 publications

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“…The first reports of silicon physiological role have shown that it is involved in the early stage of bone calcification [23,32]. Silicate bioactive glasses is osteoinductive and bonds with the surrounding tissue because of the interaction of the Si-OH groups with calcium and phosphate ions, resulting in the crystallization of apatite crystals, cell adhesion and collagen formation [32][33][34]. The excellent biocompatibility and the positive biological properties of silicate bioactive glasses have maintained them in the center of biomaterials research for many decades [35,36].…”

Section: Introductionmentioning

confidence: 99%

A Comparison of the Effects of Silica and Hydroxyapatite Nanoparticles on Poly(ε-caprolactone)-Poly(ethylene glycol)-Poly(ε-caprolactone)/Chitosan Nanofibrous Scaffolds for Bone Tissue Engineering

Hokmabad

Davaran

Aghazadeh

et al. 2018

Tissue Eng Regen Med

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BACKGROUND: The major challenge of tissue engineering is to develop constructions with suitable properties which would mimic the natural extracellular matrix to induce the proliferation and differentiation of cells. Poly(e-caprolactone)poly(ethylene glycol)-poly(e-caprolactone) (PCL-PEG-PCL, PCEC), chitosan (CS), nano-silica (n-SiO 2) and nano-hydroxyapatite (n-HA) are biomaterials successfully applied for the preparation of 3D structures appropriate for tissue engineering. METHODS: We evaluated the effect of n-HA and n-SiO 2 incorporated PCEC-CS nanofibers on physical properties and osteogenic differentiation of human dental pulp stem cells (hDPSCs). Fourier transform infrared spectroscopy, field emission scanning electron microscope, transmission electron microscope, thermogravimetric analysis, contact angle and mechanical test were applied to evaluate the physicochemical properties of nanofibers. Cell adhesion and proliferation of hDPSCs and their osteoblastic differentiation on nanofibers were assessed using MTT assay, DAPI staining, alizarin red S staining, and QRT-PCR assay. RESULTS: All the samples demonstrated bead-less morphologies with an average diameter in the range of 190-260 nm. The mechanical test studies showed that scaffolds incorporated with n-HA had a higher tensile strength than ones incorporated with n-SiO 2. While the hydrophilicity of n-SiO 2 incorporated PCEC-CS nanofibers was higher than that of samples enriched with n-HA. Cell adhesion and proliferation studies showed that n-HA incorporated nanofibers were slightly superior to n-SiO 2 incorporated ones. Alizarin red S staining and QRT-PCR analysis confirmed the osteogenic differentiation of hDPSCs on PCEC-CS nanofibers incorporated with n-HA and n-SiO 2. CONCLUSION: Compared to other groups, PCEC-CS nanofibers incorporated with 15 wt% n-HA were able to support more cell adhesion and differentiation, thus are better candidates for bone tissue engineering applications.

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Section: Introductionmentioning

confidence: 99%

A Comparison of the Effects of Silica and Hydroxyapatite Nanoparticles on Poly(ε-caprolactone)-Poly(ethylene glycol)-Poly(ε-caprolactone)/Chitosan Nanofibrous Scaffolds for Bone Tissue Engineering

Hokmabad

Davaran

Aghazadeh

et al. 2018

Tissue Eng Regen Med

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“…Calcium phosphate and calcium hydroxide are used frequently in the field of dentistry, orthopedics, and plastic surgery [ 42 – 44 ] but due to their slow degradability, not found suitable in comparison to osteoconductive HA and its derivatives [ 45 – 47 ]. The poor processibility and mechanical strength of these ceramics including silica have decreased their suitability in the fabrication of the scaffolds; hence, these bioceramics are blended with various synthetic biomaterials, such as poly(lactic acid), poly(glycolic acid), poly(lactic-co-glycolic acid), and poly(ε-caprolactone), for the fabrication of scaffolds for tissue engineering [ 48 , 49 ]. Due to the lack of cell recognition sites on synthetic polymers, the blending of collagen with synthetic polymers is found to be useful [ 50 ].…”

Section: Introductionmentioning

confidence: 99%

Micro/Nano Multilayered Scaffolds of PLGA and Collagen by Alternately Electrospinning for Bone Tissue Engineering

et al. 2016

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The dual extrusion electrospinning technique was used to fabricate multilayered 3D scaffolds by stacking microfibrous meshes of poly(lactic acid-co-glycolic acid) (PLGA) in alternate fashion to micro/nano mixed fibrous meshes of PLGA and collagen. To fabricate the multilayered scaffold, 35 wt% solution of PLGA in THF-DMF binary solvent (3:1) and 5 wt% solution of collagen in hexafluoroisopropanol (HFIP) with and without hydroxyapatite nanorods (nHA) were used. The dual and individual electrospinning of PLGA and collagen were carried out at flow rates of 1.0 and 0.5 mL/h, respectively, at an applied voltage of 20 kV. The density of collagen fibers in multilayered scaffolds has controlled the adhesion, proliferation, and osteogenic differentiation of MC3T3-E1 cells. The homogeneous dispersion of glutamic acid-modified hydroxyapatite nanorods (nHA-GA) in collagen solution has improved the osteogenic properties of fabricated multilayered scaffolds. The fabricated multilayered scaffolds were characterized using FT-IR, X-ray photoelectron spectroscopy, and transmission electron microscopy (TEM). The scanning electron microscopy (FE-SEM) was used to evaluate the adhesion and spreads of MC3T3-E1 cells on multilayered scaffolds. The activity of MC3T3-E1 cells on the multilayered scaffolds was evaluated by applying MTT, alkaline phosphatase, Alizarin Red, von Kossa, and cytoskeleton F-actin assaying protocols. The micro/nano fibrous PLGA-Col-HA scaffolds were found to be highly bioactive in comparison to pristine microfibrous PLGA and micro/nano mixed fibrous PLGA and Col scaffolds.

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“…ALP catalyzes the cleavage of organic phosphate esters and plays an important role during the formation process of bone nodule [ 1 , 2 ]. ALP activity is considered as an early marker of osteoblastic differentiation because it significantly increases during the differentiation step.…”

Section: Resultsmentioning

confidence: 99%

“…The mineralized bone nodules are usually formed via three main stages such as cell proliferation, ECM development, and mineralization. Therefore, the osteogenesis capability of the cells as a late-stage marker for osteogenic differentiation can be estimated by the determination of bone-like calcium deposits produced on different PVDF composite nanofibers using ARS staining [ 1 , 4 ]. After the incubation for 3, 5, 7, and 14 days, the absorbance changes of ARS in the red-stained composite nanofibers were used to quantify the calcium mineralization.…”

Section: Resultsmentioning

confidence: 99%

“…A variety of techniques have been tried for the regeneration of bone defects, including bone grafting, distraction osteogenesis, and guided bone regeneration (GBR) [ 1 , 2 , 3 ]. Among them, GBR has been used as an efficient method for both the reconstruction of the structure of, and reestablishment of the function in, bone defects and damaged tissues.…”

Section: Introductionmentioning

confidence: 99%

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Poly(vinylidene fluoride) Composite Nanofibers Containing Polyhedral Oligomeric Silsesquioxane–Epigallocatechin Gallate Conjugate for Bone Tissue Regeneration

et al. 2019

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To provide adequate conditions for the regeneration of damaged bone, it is necessary to develop piezoelectric porous membranes with antioxidant and anti-inflammatory activities. In this study, composite nanofibers comprising poly(vinylidene fluoride) (PVDF) and a polyhedral oligomeric silsesquioxane–epigallocatechin gallate (POSS–EGCG) conjugate were fabricated by electrospinning methods. The resulting composite nanofibers showed three-dimensionally interconnected porous structures. Their average diameters, ranging from 936 ± 223 nm to 1094 ± 394 nm, were hardly affected by the addition of the POSS–EGCG conjugate. On the other hand, the piezoelectric β-phase increased significantly from 77.4% to 88.1% after adding the POSS–EGCG conjugate. The mechanical strength of the composite nanofibers was ameliorated by the addition of the POSS–EGCG conjugate. The results of in vitro bioactivity tests exhibited that the proliferation and differentiation of osteoblasts (MC3T3-E1) on the nanofibers increased with the content of POSS–EGCG conjugate because of the improved piezoelectricity and antioxidant and anti-inflammatory properties of the nanofibers. All results could suggest that the PVDF composite nanofibers were effective for guided bone regeneration.

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Physical and bioactive properties of multi-layered PCL/silica composite scaffolds for bone tissue regeneration

Cited by 35 publications

References 40 publications

A Comparison of the Effects of Silica and Hydroxyapatite Nanoparticles on Poly(ε-caprolactone)-Poly(ethylene glycol)-Poly(ε-caprolactone)/Chitosan Nanofibrous Scaffolds for Bone Tissue Engineering

A Comparison of the Effects of Silica and Hydroxyapatite Nanoparticles on Poly(ε-caprolactone)-Poly(ethylene glycol)-Poly(ε-caprolactone)/Chitosan Nanofibrous Scaffolds for Bone Tissue Engineering

Micro/Nano Multilayered Scaffolds of PLGA and Collagen by Alternately Electrospinning for Bone Tissue Engineering

Poly(vinylidene fluoride) Composite Nanofibers Containing Polyhedral Oligomeric Silsesquioxane–Epigallocatechin Gallate Conjugate for Bone Tissue Regeneration

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