Micro/Nano Multilayered Scaffolds of PLGA and Collagen by Alternately Electrospinning for Bone Tissue Engineering

Kwak, Sanghwa; Haider, Adnan; Gupta, K. C.; Kim, Sukyoung; Kang, Inn‐Kyu

doi:10.1186/s11671-016-1532-4

Cited by 74 publications

(41 citation statements)

References 74 publications

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“…Composites made of collagen and hydroxyapatite (HA) are attractive biocompatible materials as they possess the organic and mineral constituents of bone [46][47][48]. Scaffolds made of collagen and HA with/without synthetic polymers have been widely produced by electrospinning (ES) [49][50][51][52] and conventional scaffold fabrication techniques [53][54][55][56][57]. The flexibility of generating broad pore size ranges and mechanical properties by additive manufacturing (AM) makes it a superior alternative to ES and conventional techniques [58][59][60][61].…”

Section: Discussionmentioning

confidence: 99%

Validation of scaffold design optimization in bone tissue engineering: finite element modeling versus designed experiments

et al. 2017

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This study reports the development of biological/synthetic scaffolds for bone tissue engineering (TE) via 3D bioplotting. These scaffolds were composed of poly(L-lactic-co-glycolic acid) (PLGA), type I collagen, and nano-hydroxyapatite (nHA) in an attempt to mimic the extracellular matrix of bone. The solvent used for processing the scaffolds was 1,1,1,3,3,3-hexafluoro-2-propanol. The produced scaffolds were characterized by scanning electron microscopy, microcomputed tomography, thermogravimetric analysis, and unconfined compression test. This study also sought to validate the use of finite-element optimization in COMSOL Multiphysics for scaffold design. Scaffold topology was simplified to three factors: nHA content, strand diameter, and strand spacing. These factors affect the ability of the scaffold to bear mechanical loads and how porous the structure can be. Twenty four scaffolds were constructed according to an I-optimal, split-plot designed experiment (DE) in order to generate experimental models of the factor-response relationships. Within the design region, the DE and COMSOL models agreed in their recommended optimal nHA (30%) and strand diameter (460 μm). However, the two methods disagreed by more than 30% in strand spacing (908 μm for DE; 601 μm for COMSOL). Seven scaffolds were 3D-bioplotted to validate the predictions of DE and COMSOL models (4.5-9.9 MPa measured moduli). The predictions for these scaffolds showed relative agreement for scaffold porosity (mean absolute percentage error of 4% for DE and 13% for COMSOL), but were substantially poorer for scaffold modulus (51% for DE; 21% for COMSOL), partly due to some simplifying assumptions made by the models. Expanding the design region in future experiments (e.g., higher nHA content and strand diameter), developing an efficient solvent evaporation method, and exerting a greater control over layer overlap could allow developing PLGA-nHA-collagen scaffolds to meet the mechanical requirements for bone TE.

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Section: Discussionmentioning

confidence: 99%

Validation of scaffold design optimization in bone tissue engineering: finite element modeling versus designed experiments

et al. 2017

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“…Demonstrating that the homogeneous dispersion of glutamic acid-modified hydroxyapatite nanoparticles (nHA-GA) in the COL solution improved the osteogenic properties of the multilayer scaffolds fabricated. In addition, it found that PLGA-COL-HA micro-nano fibrous scaffolds were highly bioactive compared to pristine microfibrous PLGA and PLGA and COL micro/nano-mixed fibrous platforms [26].…”

Section: Collagen Electrospinningmentioning

confidence: 97%

Gelatin and Collagen Nanofiber Scaffolds for Tissue Engineering

Monroy¹,

Bravo²,

Mercado³

et al. 2018

Tissue Regeneration

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One of the main complications that can present a person with second and third degree burns is the possibility of being infected by opportunistic bacteria or viruses that are present in the environment. Nowadays, the majority of the burn injuries are treated with conventional gauze, which involves a high probability of infection and pain for the patient being treated with this method. In order to obtain low-cost scaffolds, natural and abundant polymers were used such as gelatin (GEL) and collagen (COL). The GEL functions as a base scaffold, stable and flexible, and also biocompatible because it is a byproduct of the partial hydrolysis of COL, which is an indispensable component for the stability of the cell membrane and it is present in great extent in the human epithelium.

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“…Designing the hierarchical micro/nanoscaled scaffolds for tissue generation could (1) modulate the functional response of cell; (2) induce structural and functional integration of cells and tissues; (3) control the process of tissue remodeling by the structure and component modulation of hierarchical network. Recently, considerable research efforts have been made to develop various hierarchical micro/nanostructured scaffolds for bone repair, Achilles tendons, rotator cuffs, and skin tissue . Dura mater is a connective tissue membrane composed mainly of hierarchical collagen fibers with various diameters, multilayers, and orientations .…”

Section: Introductionmentioning

confidence: 99%

Hierarchical Micro/Nanofibrous Bioscaffolds for Structural Tissue Regeneration

Cui

Zhang

et al. 2017

Adv Healthcare Materials

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Various biomimetic scaffolds with hierarchical micro/nanostructures are designed to closely mimic native extracellular matrix network and to guide cell behavior to promote structural tissue generation. However, it remains a challenge to fabricate hierarchical micro/nanoscaled fibrous scaffolds with different functional components that endow the scaffolds with both biochemical and physical features to exert different biological roles during the process of tissue healing. In this study, a biomimetic designed micro/nanoscaled scaffold with integrated hierarchical dual fibrillar components is fabricated in order to repair dura mater and prevent the formation of epidural scars via collagen molecule self-assembly, electrospinning, and biological interface crosslinking strategies. The fabricated biomimetic scaffolds display micro/nanofibers staggered hierarchical architecture with good mechanical properties and biocompatibility, and it has a more profound effect on attachment, proliferation, and differentiation of fibroblasts. Using a rabbit duraplasty model in vivo, the authors find that dural defects repaired with hierarchical micro/nanoscaled scaffold form a continuous neodura tissue similar to native dura mater; furthermore, the number of scar tissues decreases significantly in the laminectomy sites compared with conventional electrospun microfibrous scaffold. Taken together, these data suggest that the hierarchical micro/nanoscaled fibrous scaffolds with dual fibrillar components may act as a "true" dural substitutes for dual repair.

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Micro/Nano Multilayered Scaffolds of PLGA and Collagen by Alternately Electrospinning for Bone Tissue Engineering

Cited by 74 publications

References 74 publications

Validation of scaffold design optimization in bone tissue engineering: finite element modeling versus designed experiments

Validation of scaffold design optimization in bone tissue engineering: finite element modeling versus designed experiments

Gelatin and Collagen Nanofiber Scaffolds for Tissue Engineering

Hierarchical Micro/Nanofibrous Bioscaffolds for Structural Tissue Regeneration

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