Photoprotective properties of a hydrophilic extract of the fern Polypodium leucotomos on human skin cells

Alonso-Lebrero, José Luis; Domínguez‐Jiménez, Carmen; Tejedor, Reyes; Brieva, Aurora; Pivel, J.P.

doi:10.1016/s1011-1344(03)00051-4

Cited by 51 publications

(36 citation statements)

References 23 publications

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“…Moreover, exposure to both these types of radiation also increased cell death. Previous studies have revealed the ability of P. leucotomos to preserve human fibroblasts after UVA light exposure [25] and to prevent the epidermal cellular damage caused by UVB light [26]. In this work we wanted to explore whether P. leucotomos extract was also able to prevent cell damage caused by VIS and wIRA radiation.…”

Section: Discussionmentioning

confidence: 99%

Fernblock Prevents Dermal Cell Damage Induced by Visible and Infrared A Radiation

Zamarrón

Lorrio

González

et al. 2018

IJMS

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Sun overexposure leads to higher risk of photoaging and skin cancer. The contribution of infrared (IR) and visible light (VIS) radiation is currently being taken into account in their pathogenesis. Erythema, hyperpigmentation, genotoxicity or the increase of matrix metalloproteinases (MMPs) expression are some of the effects induced by these types of radiation. Extracts of various botanicals endowed with antioxidant activity are emerging as new photoprotective compounds. A natural extract from Polypodium leucotomos (Fernblock®, FB) has antioxidant and photoprotective properties and exhibits a strong anti-aging effect. In this study, we evaluated the protective capacity of FB against the detrimental effects of infrared A (IRA) and VIS radiation in human dermal fibroblasts. We analyzed the effects of FB on the morphology, viability, cell cycle and expression of extracellular matrix components of fibroblasts subjected to VIS and IRA. Our results indicate that FB prevents cell damage caused by VIS and IRA. Moreover, it reduces the increase in MMP-1 and cathepsin K expression induced by both VIS and IRA radiation, and curbs alterations in fibrillin 1, fibrillin 2 and elastin expression. All these findings support FB as a feasible approach to prevent or treat skin damage caused by IRA or VIS exposure.

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Section: Discussionmentioning

confidence: 99%

Fernblock Prevents Dermal Cell Damage Induced by Visible and Infrared A Radiation

Zamarrón

Lorrio

González

et al. 2018

IJMS

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show abstract

“…35,36 In addition, PL has been reported to prevent the loss of cell viability (apoptosis) and proliferation induced by UVA. 37 Moreover, PL has also been proven to reduce inflammation through down-regulation of tumor necrosis factor-␣. 38 Our present findings support and significantly extend the current knowledge of PL's photoprotective effects.…”

Section: Discussionmentioning

confidence: 99%

Polypodium leucotomos Extract Decreases UV-Induced Cox-2 Expression and Inflammation, Enhances DNA Repair, and Decreases Mutagenesis in Hairless Mice

Zattra

Coleman

Arad

et al. 2009

The American Journal of Pathology

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mice were fed for 10 days with PL (300 mg/kg) or vehicle then UV-irradiated, once. By 24 hours, UVinduced Cox-2 levels were increased in vehicle-fed and PL-fed mice, whereas by 48 and 72 hours, Cox-2 levels were four-to fivefold lower in PL-fed mice (P < 0.05). p53 expression/activity was increased in PL-fed versus vehicle-fed then UV-irradiated mice. UV-induced inflammation was decreased in PL-fed mice, as shown by ϳ60% decrease (P < 0.001) in neutrophil infiltration at 24 hours, and macrophages by ϳ50% (<0.02) at 24 and 48 hours. By 72 hours, 54 ؎ 5% cyclobutane pyrimidine dimers remained in vehiclefed versus 31 ؎ 5% in PL-fed skin (P < 0.003). The number of 8-hydroxy-2-deoxyguanosine-positive cells were decreased before UV irradiation by ϳ36% (P < 0.01), suggesting that PL reduces constitutive oxidative DNA damage. By 6 and 24 hours, the number of 8-hydroxy-2-deoxyguanosine-positive cells were ϳ59% (P < 0.01) and ϳ79% (P < 0.03) lower in PL-fed versus vehicle-fed mice. Finally, UV-induced mutations in PL-fed-mice were decreased by ϳ25% when assessed 2 weeks after the single UV exposure. These data demonstrate that PL extract supplementation affords the following photoprotective effects: p53 activation and reduction of acute inflammation via Cox-2 enzyme inhibition, increased cyclobutane pyrimidine dimer removal, and reduction of oxidative DNA damage.

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“…As a whole, oral pretreatment with PL demonstrated a protective effect against UVR‐mediated, ROS‐dependent phenomena, both in epidermis and in blood. Previous studies have underscored the efficacy of PL in counteracting the deleterious effects of UVR in in vitro cell culture systems (10,20) and after topical application in in vivo models (21,22). Most interestingly, oral administration of PL in humans significantly decreased erythema formation and the number of sunburn cells, and also reduced pyrimidine dimers formation upon exposure to UVR (23).…”

Section: Discussionmentioning

confidence: 99%

Polypodium leucotomos extract inhibits glutathione oxidation and prevents Langerhans cell depletion induced by UVB/UVA radiation in a hairless rat model

Mulero¹,

Rodríguez-Yanes²,

Nogués³

et al. 2008

Experimental Dermatology

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In this report, we have addressed the effect of oral administration of a hydrophilic extract of the fern Polypodium leucotomos (PL) on the deleterious effects of ultraviolet radiation (UVR) on the levels of epidermal and plasmatic antioxidants in hairless rats. We have found that pretreatment with PL effectively reduced glutathione oxidation in both blood and epidermis, suggesting a potent systemic antioxidant effect. In addition, PL inhibited UVR-mediated Langerhans cell (LC) depletion. Our results demonstrate the efficacy of PL as an oral antioxidant and photoimmunoprotective agent and support its employment as a complement to topical sunscreens.

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Photoprotective properties of a hydrophilic extract of the fern Polypodium leucotomos on human skin cells

Cited by 51 publications

References 23 publications

Fernblock Prevents Dermal Cell Damage Induced by Visible and Infrared A Radiation

Fernblock Prevents Dermal Cell Damage Induced by Visible and Infrared A Radiation

Polypodium leucotomos Extract Decreases UV-Induced Cox-2 Expression and Inflammation, Enhances DNA Repair, and Decreases Mutagenesis in Hairless Mice

Polypodium leucotomos extract inhibits glutathione oxidation and prevents Langerhans cell depletion induced by UVB/UVA radiation in a hairless rat model

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