Polypodium leucotomos Extract Decreases UV-Induced Cox-2 Expression and Inflammation, Enhances DNA Repair, and Decreases Mutagenesis in Hairless Mice

Abstract

mice were fed for 10 days with PL (300 mg/kg) or vehicle then UV-irradiated, once. By 24 hours, UVinduced Cox-2 levels were increased in vehicle-fed and PL-fed mice, whereas by 48 and 72 hours, Cox-2 levels were four-to fivefold lower in PL-fed mice (P < 0.05). p53 expression/activity was increased in PL-fed versus vehicle-fed then UV-irradiated mice. UV-induced inflammation was decreased in PL-fed mice, as shown by ϳ60% decrease (P < 0.001) in neutrophil infiltration at 24 hours, and macrophages by ϳ50% (<0.02) at 24 and 48 hours. By 72 hours, 54 ؎ 5% cyclobutane pyrimidine dimers remained in vehiclefed versus 31 ؎ 5% in PL-fed skin (P < 0.003). The number of 8-hydroxy-2-deoxyguanosine-positive cells were decreased before UV irradiation by ϳ36% (P < 0.01), suggesting that PL reduces constitutive oxidative DNA damage. By 6 and 24 hours, the number of 8-hydroxy-2-deoxyguanosine-positive cells were ϳ59% (P < 0.01) and ϳ79% (P < 0.03) lower in PL-fed versus vehicle-fed mice. Finally, UV-induced mutations in PL-fed-mice were decreased by ϳ25% when assessed 2 weeks after the single UV exposure. These data demonstrate that PL extract supplementation affords the following photoprotective effects: p53 activation and reduction of acute inflammation via Cox-2 enzyme inhibition, increased cyclobutane pyrimidine dimer removal, and reduction of oxidative DNA damage.

PROTECTIVE EFFECT ETHANOLIC EXTRACT OF Boesenbergia pandurata (ROXB.) Schlecht. AGAINST UVB-INDUCED DNA DAMAGES IN BALB/C MICE

“…These CPDs + cells persisted up to 72h post-UVB. This finding supported the previously reported studies on UV inducedhairless mice (Zattra et al, 2009).…”

“…This result were in accordance with previous studies, which indicated that natural diet agents including fruits, vegetables, and spices had ability to repress UV-induced skin cancer through remove or decrease the DNA damage. Acceleration of UV-induced CPDs removal were demonstrated by other natural compounds from extracts of Polypodium leucotomos (Zattra et al, 2009), Punica granatum fruits (Afag et al, 2010), green tea polyphenol Roche et al, 2010), and luteolin (Wolfle et al, 2011). The mechanism of decreasing CPDs might be through antioxidant activity, increasing IL-12 (cytokine interfere formation of CPDs), p53 and p21 protein (Katiyar, 2007;Zattra et al, 2009;Afag et al, 2010;Wolfle et al, 2011).…”

“…Acceleration of UV-induced CPDs removal were demonstrated by other natural compounds from extracts of Polypodium leucotomos (Zattra et al, 2009), Punica granatum fruits (Afag et al, 2010), green tea polyphenol Roche et al, 2010), and luteolin (Wolfle et al, 2011). The mechanism of decreasing CPDs might be through antioxidant activity, increasing IL-12 (cytokine interfere formation of CPDs), p53 and p21 protein (Katiyar, 2007;Zattra et al, 2009;Afag et al, 2010;Wolfle et al, 2011). B. pandurata rhizome has been reported contain essential oil, pinostrobin, alpinetin, pinocembrin, cardamonin, boesenbergin, panduratin, and others (Ching et al, 2007), they might be contribute and affect on signaling pathway of DNA repair activity and therefore could decrease, inhibit and remove formation of CPDs.…”

Scoping systematic review of treatments for eczema

“…Laboratory studies have demonstrated that P. leucotomos extracts have antioxidant and photoprotective properties. 445 …”

Sunscreens and Antioxidants as Photo-protective Measures: An update

“…* Polypodium leucotomos extract (PL) can also be administered orally with very low toxicity, in addition to its topical use as already described.Oral PL scavenges free radicals and reactive oxygen species such as superoxide anion, singlet oxygen, hydroxyl radical and hydrogen peroxide, and prevents lipid peroxidation [47,48]. Besides the effects already mentioned, PL also prevents oxidative DNA damage (8-hydroxyguanine) and accelerates repair of thymine dimers [24,49]. In addition, it also inhibitstrans-urocanic acid photo-induced isomerization and inactivation, as well as UVA-induced cyclobutane pyrimidine dimer deletions and mitochondrial DNA damage [50,51].…”