Photodynamic Antimicrobial Chemotherapy (PACT), using Toluidine blue O inhibits the viability of biofilm produced by Candida albicans at different stages of development

Pinto, Ana Paula; Rosseti, Isabela Bueno; Carvalho, Moisés Lopes; Silva, Bruna Marques da; Alberto-Silva, Carlos; Costa, Maricília Silva

doi:10.1016/j.pdpdt.2017.12.001

Cited by 40 publications

(24 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Another study by Pinto et al, 23 that studied the effects of photoinactivation on the age of biofilm C. albicans (6,12,24 and 48 hours), used exogenous photosensitizer of toluidine blue O and sources light of LED (630 nm, 0.073 W, beam area 0.38 cm 2 , energy density 21.7 J/cm 2 ). The morphology of biofilm is change from yeast to hype happen at 12 hours growth with the value of metabolic activity after irradiation were 22% and 48% for the concentrations of Toluidine Blue O are 0.05 and 0.1 mg/ mL, respectively.…”

Section: Discussionmentioning

confidence: 99%

The Efficacy of Photodynamic Inactivation of the Diode Laser in Inactivation of the Candida albicans Biofilms With Exogenous Photosensitizer of Papaya Leaf Chlorophyll

et al. 2019

View full text Add to dashboard Cite

Introduction: Photodynamic inactivation has been developed to kill pathogenic microbes. In addition, some techniques have been introduced to minimize the biofilm resistance to antifungal properties in inhibiting cell growth. The principle of photodynamic inactivation different to antifungal drugs therapy which is resistant to biofilms. The presence of reactive oxygen species (ROS) that generating in photodynamic inactivation mechanisms can be damaging of biofilm cells and the principle of light transmission that could be penetrating in matrix layers of extracellular polymeric substance (EPS) until reaching the target cells at the base layers of biofilm. The present work aims to explore the potential of chlorophyll extract of papaya leaf as an exogenous photosensitizer to kill the Candida albicans biofilms after being activated by the laser. The potential of chlorophyll photosensitizer was evaluated based on the efficacy of inactivation C. albicans biofilm cell through a cell viability test and an organic compound test. Methods: The treatment of photoinactivation was administered to 12 groups of C. albicans biofilm for four days using the 445 nm laser and the 650 nm laser. The 445 nm and 650 nm lasers activated the chlorophyll extract of the papaya leaf (0.5 mg/L) at the same energy density. The energy density variation was determined as 5, 10, 20, 30 and 40 J/cm2 with the duration of exposure of each laser adjusted to the absorbance percentage of chlorophyll extract of the papaya leaf. Results: The absorbance percentage of chlorophyll extracts of the papaya leaf on wavelengths of 650 nm and 445 nm respectively were 22.26% and 60.29%, respectively. The most effective treated group was a group of the laser with the addition of chlorophyll, done by the 650 nm lasers with inactivation about 32% (P=0.001), while the 445 nm lasers only 25% (P=0.061). The maximum malondialdehyde levels by treatment of the laser 650 nm were (0.046±0.004) nmol/mg. Conclusion: The use of chlorophyll extract of the papaya leaf as a photosensitizer, resulted in the maximum spectrum of absorption at 414 nm and 668 nm, which produced a maximum reduction effect after photoinactivation up to 32% (with chlorophyll) and 25% (without chlorophyll). The utilization of chlorophyll extract of the papaya leaf would increase the antifungal effects with the activation by the diode laser in the biofilm of C. albicans

show abstract

Section: Discussionmentioning

confidence: 99%

The Efficacy of Photodynamic Inactivation of the Diode Laser in Inactivation of the Candida albicans Biofilms With Exogenous Photosensitizer of Papaya Leaf Chlorophyll

et al. 2019

View full text Add to dashboard Cite

show abstract

“…The technique led to photoinactivation of the biofilm and reduced colony count and metabolic activity [44]. Similar effects were obtained using hypericin-glucamine [45], Rose Bengal in α-cyclodextrin [46], curcumin [44,[47][48][49][50], methylene blue [52], toluidine blue O [51], erythrosine with green light [55], or Photodithazine ® obtained from the cyanobacterium Spirulina platensis [53,54]. Another study conducted by Soria-Lozano et al [80], using Rose Bengal, methylene blue, and curcumin with white light, showed positive effects against the S. mutans and S. sanguis strains.…”

Section: Chemical Compounds With Activity Against Streptococcus Mutanmentioning

confidence: 67%

Management of Streptococcus mutans-Candida spp. Oral Biofilms’ Infections: Paving the Way for Effective Clinical Interventions

Salehi

Kręgiel

Mahady

et al. 2020

JCM

View full text Add to dashboard Cite

Oral diseases are considered the most common noncommunicable diseases and are related to serious local and systemic disorders. Oral pathogens can grow and spread in the oral mucosae and frequently in biomaterials (e.g., dentures or prostheses) under polymicrobial biofilms, leading to several disorders such as dental caries and periodontal disease. Biofilms harbor a complex array of interacting microbes, increasingly unapproachable to antimicrobials and with dynamic processes key to disease pathogenicity, which partially explain the gradual loss of response towards conventional therapeutic regimens. New drugs (synthesized and natural) and other therapies that have revealed promising results for the treatment or control of these mixed biofilms are presented and discussed here. A structured search of bibliographic databases was applied to include recent research. There are several promising new approaches in the treatment of Candida spp.–Streptococcus mutans oral mixed biofilms that could be clinically applied in the near future. These findings confirm the importance of developing effective therapies for oral Candida–bacterial infections.

show abstract

“…Studies show that aPDT treatment of biofilms requires longer incubation times and higher TBO concentrations, which results in improved efficacy visible as a reduction of the number of cells in both the yeast and filamentous forms [35,36] and a significant reduction in the mass of EPS (LED 635 nm, fluence 50 J/cm 2 , concentration of TBO 2.5 mM, PIT 30 min) [37]. In other studies, using planktonic solutions and biofilms of Candida strains with TBO-mediated aPDT, the PIT was not a variable of the protocol; the authors used 30 s to 180 min with the most common of 5 min (nine studies) [25,32,33,35,36,[38][39][40][41] or 30 min (seven studies) [24,33,37,[42][43][44][45]. Other incubation times used in that study were 1 min [46,47], 10 min [48] and 20 min [29].…”

Section: Discussionmentioning

confidence: 99%

Influence of Incubation Time on Ortho-Toluidine Blue Mediated Antimicrobial Photodynamic Therapy Directed against Selected Candida Strains—An In Vitro Study

Wiench

Nowicka

Pajączkowska

et al. 2021

IJMS

View full text Add to dashboard Cite

(1) Background and the aim: The appropriate incubation time in the antimicrobial photodynamic therapy protocol seems to have a huge impact on the efficacy of this process. This is particularly important in relation to Candida strains, due to the size of these cells and the presence of the cell wall. The aims of this study were to determine the optimal incubation time needed for the absorption of toluidine blue by cells of C. albicans, C. glabrata, C. krusei and C. parapsilosis using direct observation by optical microscopy, and to evaluate the efficacy of TBO-mediated aPDT on planktonic cells of these strains. (2) Methods: The microscopic evaluation consisted of taking a series of images at a magnification of 600× and counting the % of stained cells. The in vitro effect of TBO-mediated aPDT combined with a diode laser (635 nm, 400mW, 12 J/cm2, CW) on the viability of yeast cells with different incubation times was evaluated. (3) Results: The presence of TBO within the cytoplasm was observed in all tested Candida strains and at all microscopic evaluation times. However, the highest percentages of cells were stained at 7 and 10 min. The highest % reduction of CFU/mL after TBO-mediated aPDT against Candida was obtained for the strain C. albicans ATCC 10,231 and it was 78.55%. (4) Conclusions: TBO-mediated aPDT against Candida was effective in reducing the number of CFU/mL at all assessed incubation times. However, the most efficient period for almost all strains was 7–10 min.

show abstract

Photodynamic Antimicrobial Chemotherapy (PACT), using Toluidine blue O inhibits the viability of biofilm produced by Candida albicans at different stages of development

Cited by 40 publications

References 64 publications

The Efficacy of Photodynamic Inactivation of the Diode Laser in Inactivation of the Candida albicans Biofilms With Exogenous Photosensitizer of Papaya Leaf Chlorophyll

The Efficacy of Photodynamic Inactivation of the Diode Laser in Inactivation of the Candida albicans Biofilms With Exogenous Photosensitizer of Papaya Leaf Chlorophyll

Management of Streptococcus mutans-Candida spp. Oral Biofilms’ Infections: Paving the Way for Effective Clinical Interventions

Influence of Incubation Time on Ortho-Toluidine Blue Mediated Antimicrobial Photodynamic Therapy Directed against Selected Candida Strains—An In Vitro Study

Contact Info

Product

Resources

About