Photobiomodulation with single and combination laser wavelengths on bone marrow mesenchymal stem cells: proliferation and differentiation to bone or cartilage

Abstract

Background: Tissue engineering aims to take advantage of the ability of undifferentiated stem cells to differentiate into multiple cell types to repair damaged tissue. Photobiomodulation uses either lasers or light-emitting diodes to promote stem cell proliferation and differentiation. The present study aimed to investigate single and dual combinations of laser wavelengths on mesenchymal stem cells (MSCs). Materials and Methods: MSCs were derived from rabbit iliac bone marrow. One control and 8 laser irradiated groups were designated as: Infra-red (IR, 810nm), Red (R, 660 nm), Green (G, 532 nm), Blue (B, 485 nm), IR-R, IR-B, R-G, B-G. Irradiation was repeated daily for 21 days and cell proliferation, osseous or cartilaginous differentiation was then measured. RT-PCR biomarkers were SOX9, aggrecan, COL 2 and COL 10 expression for cartilage and ALP, COL 1 and osteocalcin expression for bone. Results: Cellular proliferation was increased in all irradiated groups except G. All cartilage markers were significantly increased by IR and IR-B except COL 10 which was suppressed by IR-B combination. ALP expression was highest in R and IR groups during osseous differentiation.

Effects of photobiomodulation on bone defects grafted with bone substitutes: A systematic review of in vivo animal studies

“…Interestingly, in this context, a study by Soares et al [62] has shown that combined therapy of PBM laser (λ 780 nm) and LED (λ 850 ± 10 nm) has synergistically enhanced regeneration of bone defects grafted with or with biphasic synthetic HA + β-TCA. Hence, a recent in vitro study by Fekrazad et al [97] evaluated the effects single and combination laser wavelengths at the same fluence of 4 J/cm 2 (λ 810, 660, 485, 532nm, 810 + 485 nm, 660 + 532 nm, 810 + 660 nm, 532 + 485 nm) on bone marrow MSCs (BMSCs). It concluded that single wavelength of λ 810nm or λ 660nm has stimulated proliferation of BMSCs and modulated into new bone, however, λ 532nm laser inhibited the cells proliferation.…”

Under the spotlight: mechanisms of photobiomodulation concentrating on blue and green light

“…A second Scopus database search was undertaken to evaluate the effects of green light PBM using the terms described in Figure 3b. An initial search resulted in 236 articles being identified and these articles were subsequently screened for suitability, which then identified 32 relevant articles for further review (18,32,33,64,68,69,79,164,168,171,176,177,186,197,205,209,(212)(213)(214)(215)(216)(217)(218)(219)(220)(221)(222)(223)(224)(225)(226)(227).…”

Comparative Effect of Low‐intensity Laser Radiation in Green and Red Spectral Regions on Functional Characteristics of Sturgeon Sperm

“…It also follows from the data presented that, when in comparative studies the equality in the energy doses of the acting radiation in red and green spectral regions was provided, the stimulatory effect of radiation in red spectral region was more pronounced (72)(73)(74)(75)(76)(77)(78)(79).…”