Phosphate Solubilization and Gene Expression of Phosphate-Solubilizing Bacterium Burkholderia multivorans WS-FJ9 under Different Levels of Soluble Phosphate

Zeng, Qingwei; Wu, Xiao‐Qin; Wang, Jiangchuan; Ding, Xiaolei

doi:10.4014/jmb.1611.11057

Cited by 59 publications

(54 citation statements)

References 48 publications

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“…The shaping of a microbial community specialized towards P cycling when P is limited was already described for forest soils [17] and other studies related low P availability to changes in soil microbial communities [52,53]. Despite of not being responsive to P addition in terms of growth, Dor-364 [Pine cient] showed an improvement of several functions involved in P metabolism when exposed to P depleted conditions.…”

Section: Rhizosphere Taxonomical Assembly Under Phosphorus Depleted Cmentioning

confidence: 85%

Phosphorus Source and Availability Modulate the Rhizosphere Bacterial Community Assembly in Common Bean

Chiaramonte

Vargas-Hoyos

Costa

et al. 2020

Preprint

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Background Phosphorus (P) availability is the main nutritional factor that limits crops yields in tropical soils due to edaphic processes that lead to P immobilization after mineral fertilization. Considering the potential of the rhizosphere microbiome to transform insoluble P into forms readily available for plant uptake, in this study is proposed that plants with contrasting P uptake efficiency, growing under depleted amounts of P are able to shape distinct bacterial communities in the rhizosphere enriching taxa specialized in P mobilization. Methods We selected two common bean genotypes contrasting in P efficiency uptake and grew them in a soil with a gradient of two different sources of P, triple superphosphate (TSP) or rock phosphate Bayovar (RPB). The rhizosphere bacterial community was assessed by 16S rRNA amplicon sequencing. Data analyses focused in describing the structure of the bacterial communities, identification of OTUs differentially enriched in different treatments, functional metagenomic prediction and cooccurrence network. Results P sources and levels resulted in different rhizosphere bacterial community structure. A high number of differentially enriched OTUs were observed under P depleted conditions in the P-inefficient genotype, mainly belonging to Actinobacteria phylum. The P-inefficient genotype did not show significant differences in the rhizosphere bacterial community assembly growing in different P sources. Predicted metagenome profiles showed the enrichment of bacterial functions involved in P mobilization, in the rhizosphere of the P inefficient genotype cultivated in P depleted conditions. The network analysis revealed that in the rhizosphere of the P-inefficient genotype under P depleted conditions the bacterial community has a higher number of nodes and edges, higher average degree and clustering coeficient when compared to the treatment with optimal P level. Conclusion Our data showed that the uptake of exogenous input resulted in the assembly of a P-competent microbiome in the P-inefficient genotype compared to the efficient one, supporting the hypothesis that the selective pressure for the P uptake engages P-inefficient genotypes in symbiotic relationships with the soil microbiome. These results will pave the way for future experimentation aiming at explore the contribution of this P-competent microbiome to plant growth and development in a range of soil type.

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Section: Rhizosphere Taxonomical Assembly Under Phosphorus Depleted Cmentioning

confidence: 85%

Phosphorus Source and Availability Modulate the Rhizosphere Bacterial Community Assembly in Common Bean

Chiaramonte

Vargas-Hoyos

Costa

et al. 2020

Preprint

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“…On the contrary, less available P in intercropping and negative correlation with microbial abundance showed that due to nitrogen fixing microbes P uptake enhanced significantly and it reduced the soil available Li et al [80] reported that intercropping reduced the Olsen P in the soil. Moreover, higher phosphate uptake enhanced the plant growth and diazotrophic microbes like Bradyrhizobium, Burkholderia and Rhizobium are well-known phosphate solubilizer [81][82][83].…”

Section: Discussionmentioning

confidence: 99%

Linkages of Soil Nutrients and Diazotrophic Microbiome under Sugarcane-Legume Intercropping

Solanki¹,

Li²,

Wang³

et al. 2018

Preprint

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Intercropping significantly improves land use efficiency and soil fertility. This study examines the impact of three cultivation systems (monoculture sugarcane, peanut-sugarcane and soybean-sugarcane intercropping) on soil properties and diazotrophs. Sugarcane rhizosphere soil was sampled from the farmers’ field. Soil properties and nifH gene abundance were analyzed by high throughput sequencing. Moreover, a total of 436,458 nifH gene sequences were obtained and classified into the 3201 unique operational taxonomic units (OTUs). Maximum unique OTUs resulted with soybean-sugarcane intercropping (<375). The dominant groups across all cultivation were Alpha-proteobacteria and Beta-proteobacteria. On the basis of microbial community structure, intercropping systems were more diverse than monoculture sugarcane. In the genus level, Bradyrhizobium, Burkholderia, Pelomonas, and Sphingomonas were predominant in the intercropping systems. Moreover, diazotrophic bacterial communities of these cultivation systems were positively correlated to the soil pH and soil enzyme protease. Moreover, low available P recovered from intercropping system showed a strong correlation with higher nutrient uptake activity of soil microbes. Based on the results, our investigation concluded that intercropping system caused a positive effect on the growth of diazotrophic bacterial communities and it might boost the soil fertility and this kind of study helps to develop an eco-friendly technology for sustainable sugarcane production.

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“…Studies have shown that the phosphate-solubilizing ability of PSB is mostly regulated by the concentration of exogenous soluble phosphate, and most of the organic acids, enzymes and other substances involved in phosphate solubilization are secreted externally to degrade insoluble phosphate (Zeng et al 2017;Geng et al 2019). On the basis of the above, this study has increased our understanding of two phosphate solubilization systems that "communicate" between the strain and the outside environment, namely, the two-component system and the bacterial secretion system.…”

Section: Fj9 Strains Under Different Exogenous Soluble Phosphate Condmentioning

confidence: 99%

“…A high-efficiency PSB from the rhizosphere of pine trees, B. multivorans WS-FJ9, was screened in our laboratory. Previous studies have shown that WS-FJ9 has good ability to promote plant growth, dissolve phosphate and antagonize a variety of plant pathogenic bacteria (Hou et al 2012), and preliminary studies have explored its solubilization mechanism of inorganic phosphate by transcriptome analysis (Zeng et al 2017). However, the ability of this strain to degrade organic phosphate and the expression levels of phosphate solubilization genes under different soluble phosphate levels are not clear.…”

Section: Introductionmentioning

confidence: 99%

Identification, cloning and expression patterns of the genes related to phosphate solubilization in Burkholderia multivorans WS-FJ9 under different soluble phosphate levels

Liu

Wang

Kong

et al. 2020

Preprint

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As important plant growth-promoting rhizobacteria, phosphate-solubilizing bacteria (PSB) fix nitrogen, dissolve potassium, promote growth, improve the soil micro-environment, and enhance soil fertility. A high-efficiency PSB strain from the pine tree rhizosphere, Burkholderia multivorans WS-FJ9, was screened in our laboratory. In this study, using a Bio Screener fully automatic microbial growth curve meter to determine the growth of the WS-FJ9 strain in phosphate-removing medium, the growth and mineral phosphate solubilization of WS-FJ9 were measured by Mo-Sb colorimetry and organophosphate-solubilization plate assays. Second-generation sequencing technology was used to obtain genomic information and to analyze possible phosphorus decomposition genes. The related expression levels of these genes under different phosphorus levels were determined by quantitative real-time PCR. The results showed that WS-FJ9 had strong adaptability and capacity for mineral phosphate solubilization at low phosphorus levels, which is characterized by its low phosphorus induction and high phosphorus inhibition. The amount of solubilized mineral phosphate could exceed 140 mg/L. The total length of the WS-FJ9 genome was 7,497,552 bp after splicing, and the GC content was 67.37%. Eight phosphate-related genes were selected to determine their expression patterns at different phosphorus levels. Among them, AP-2, GspE and GspF were only related to organic phosphorus, HlyB was only related to inorganic phosphorus, and PhoR, PhoA, AP-1 and AP-3 were related to both. The WS-FJ9 strain utilizes multiple pathways for mineral phosphate solubilization, and the solubilization processes of different phosphorus sources are interrelated and independent, indicating that the WS-FJ9 strain can adapt to different phosphorus source environments and has good potential for future applications.

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Phosphate Solubilization and Gene Expression of Phosphate-Solubilizing Bacterium Burkholderia multivorans WS-FJ9 under Different Levels of Soluble Phosphate

Cited by 59 publications

References 48 publications

Phosphorus Source and Availability Modulate the Rhizosphere Bacterial Community Assembly in Common Bean

Phosphorus Source and Availability Modulate the Rhizosphere Bacterial Community Assembly in Common Bean

Linkages of Soil Nutrients and Diazotrophic Microbiome under Sugarcane-Legume Intercropping

Identification, cloning and expression patterns of the genes related to phosphate solubilization in Burkholderia multivorans WS-FJ9 under different soluble phosphate levels

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