Phenotypic Characterization of Adherent Cells Population CD34+ CD90+ CD105+ Derived from Wharton’s Jelly

Abstract

BackgroundMesenchymal stromal cells, MSCs, show expression of specific antigens on their surface. The aim of the study is to assess the phenotype of stem cells like isolated from the umbilical cord with respect to the presence of surface antigens CD34, CD90, and CD105 and differences in the expression of surface antigens in cells isolated from freshly sampled material in comparison with the phenotype of cells from in vitro culture.Material/MethodsStem cells collected from the umbilical cord from healthy patients and then cultured in vitro. To assess the phenotype of stem cells, cytometric analysis was carried out. To assess the phenotype of cells we used fluorescently labelled monoclonal antibodies: APC Mouse anti-human CD34, PC5 Mouse anti-human CD90 and PE Mouse anti-human CD105.ResultsIn the case of cells from the umbilical cord and then cultured in vitro for the period of 10–14 days CD34 expression is lower (69,5%) in comparison with the group of cells not cultured. Not cultured cells were demonstrated 37% of cells co-expression of antigens CD34 and CD105, over 21% of CD34/CD90 cells and over 24% of CD105/CD90. Cultured cells group was showed higher percentage of CD90, CD105, CD34/CD105, CD34/CD90, CD105/CD90 in comparison with not cultured cells.ConclusionsOur reults suggested that adherent cells population from umbilical cord, demonstrate CD34 expression In vivo. Moreover, the phenotype of MSCs, mainly in the context of CD34 expression, may vary depending on the place of collection of cells and the length of growing the cell culture.

Potential Involvement of BIRC5 in Maintaining Pluripotency and Cell Differentiation of Human Stem Cells

“…Isolation of stem cells from Wharton's jelly from the umbilical cord (from 21 patients-WJC group) was performed by enzymatic digestion using the enzyme collagenase type I (Sigma, USA) [45]. Some of the stem cells from Wharton's jelly (from 39 patients) were isolated using the explant method.…”

Aldehyde dehydrogenase activity of Wharton jelly mesenchymal stromal cells: isolation and characterization

“…The phenotype of a cellular product is an important issue for optimal cell-based therapy (New et al 2015) and the phenotype of WJ-MSCs was often investigated (Van Pham et al 2016;Walecka et al 2017). Progenitors cells may display difference for CD54, 58, 106, 146 and CD200 depending of their origins (Najar et al 2010a;Najar et al 2012;Vishnubalaji et al 2012;Huang et al 2010).…”

Influence of the Type of Delivery, Use of Oxytocin, and Maternal Age on POU5F1 Gene Expression in Stem Cells Derived from Wharton’s Jelly within the Umbilical Cord

“…The study included 36 Mesenchymal stem cells were isolated from Wharton's jelly within the umbilical cords of pregnant women. The isolation was performed by enzymatic digestion using the enzyme collagenase type I (Sigma, USA); then, the stem cells were cultured for 14 days in adherent conditions [12]. After this period, the cultured cells were removed mechanically using a cellular scraper, and then, they were prepared for cytometric analysis and RNA isolation.…”