Aldehyde dehydrogenase activity of Wharton jelly mesenchymal stromal cells: isolation and characterization

Najar, Mehdi; Crompot, Emerence; Grunsven, Leo A. van; Dollé, Laurent; Lagneaux, Laurence

doi:10.1007/s10616-018-0283-8

Cited by 6 publications

(6 citation statements)

References 85 publications

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“…Transcriptomic analysis demonstrated that PAFAH1B1 was downregulated by osteogenic BMPs in MSCs [ 31 ]. Besides that, ALDH + WJ-MSCs were found to express higher osteogenic genes such as RUNX2 , OSX and OPN [ 32 ]. Nallamshetty et al [ 33 ] demonstrated that during primary MSCs’ osteoblastogenesis, ALDH1A1 was also expressed besides bone marker alkaline phosphatase (ALP).…”

Section: Discussionmentioning

confidence: 99%

Gene Expression Profiles of Human Mesenchymal Stromal Cells Derived from Wharton’s Jelly and Amniotic Membrane before and after Osteo-Induction Using NanoString Platform

Hiew

Akhir

Teoh

2022

CIMB

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The use of perinatal mesenchymal stem cells (MSCs) in bone tissue regeneration and engineering to substitute bone marrow MSCs has drawn great interest due to their high yield, ease of procurement, multilineage differentiation potential and lack of ethical concerns. Although amniotic membrane (AM) and Wharton’s jelly (WJ)-derived MSCs have been widely shown to possess osteogenic differentiation potential, the intrinsic properties determining their osteogenic capacity remain unclear. Here, we compared gene expression profiles of AM- and WJ-MSCs at basal and osteogenic conditions by using the NanoString Stem Cell Panel containing regulatory genes associated with stemness, self-renewal, Wnt, Notch and Hedgehog signalling pathways. At basal condition, WJ-MSCs displayed higher expression in most genes regardless of their functional roles in self-renewal, adhesion, or differentiation signalling pathways. After osteo-induction, elevated expression of self-renewal genes ADAR and PAFAH1B1 was observed in AM-MSCs, while stemness genes MME and ALDH1A1 were upregulated in WJ-MSC. Both MSCs showed differences in genes associated with ligands, receptors and ubiquitin ligases of the Notch pathway. In addition, further evidence was demonstrated in some signalling molecules including CTBPs, protein kinases, phosphatases, RHOA, RAC1. Downstream targets HES1 and JUN especially showed higher expression in non-induced WJ-MSCs. Hedgehog genes initially expressed in both MSCs were downregulated in WJ-MSCs during osteogenesis. This study has provided insights into the intrinsic biological differences that may lead to their discrimination in therapeutic intervention.

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Section: Discussionmentioning

confidence: 99%

Gene Expression Profiles of Human Mesenchymal Stromal Cells Derived from Wharton’s Jelly and Amniotic Membrane before and after Osteo-Induction Using NanoString Platform

Hiew

Akhir

Teoh

2022

CIMB

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“…It was shown that ALDH + sorted stem cells displayed a major hypoxia response compared to ALDH − stem cells, increasing of HIF-1α and HIF-2α. Moreover, ALDH + stem cells exhibited an increased mRNA expression of stemness correlated genes Oct-4, Nanog, Sox-2, and Rex-1 compared to ALDHcells [166].…”

Section: Hif Role In Stemnessmentioning

confidence: 94%

“…HIF capability to preserve stemness could be even correlated with aldehyde dehydrogenase (ALDH) activity as observed in Wharton's Jelly stem cells [166]. Adipose-derived stem cells [167], foreskin-derived mesenchymal stromal cells [168], and for bone marrow mesenchymal stromal cell [169].…”

Section: Hif Role In Stemnessmentioning

confidence: 99%

Insight into Hypoxia Stemness Control

Mattia

Mauro

Citeroni

et al. 2021

Cells

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Recently, the research on stemness and multilineage differentiation mechanisms has greatly increased its value due to the potential therapeutic impact of stem cell-based approaches. Stem cells modulate their self-renewing and differentiation capacities in response to endogenous and/or extrinsic factors that can control stem cell fate. One key factor controlling stem cell phenotype is oxygen (O2). Several pieces of evidence demonstrated that the complexity of reproducing O2 physiological tensions and gradients in culture is responsible for defective stem cell behavior in vitro and after transplantation. This evidence is still worsened by considering that stem cells are conventionally incubated under non-physiological air O2 tension (21%). Therefore, the study of mechanisms and signaling activated at lower O2 tension, such as those existing under native microenvironments (referred to as hypoxia), represent an effective strategy to define if O2 is essential in preserving naïve stemness potential as well as in modulating their differentiation. Starting from this premise, the goal of the present review is to report the status of the art about the link existing between hypoxia and stemness providing insight into the factors/molecules involved, to design targeted strategies that, recapitulating naïve O2 signals, enable towards the therapeutic use of stem cell for tissue engineering and regenerative medicine.

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“…Экспрессия ALDH высокая в жизнеспособных клетках-предшественницах различных линий, включая гемопоэтические, эндотелиальные, мезенхимные и нейральные [9][10][11][12]. В исследованиях последних лет показана стволовая природа мезенхимных клеток жировой ткани [13], вартонова студня пуповины [14], кожи [15], экспрессирующих ALDH. В то же время практически отсутствуют данные о количестве и динамике содержания ALDH-позитивных (ALDH+) элементов в культуре МСК костного мозга доноров и возможной взаимосвязи с показателями скорости роста культуры.…”

Section: Introductionunclassified

Aldehyde Dehydrogenase as a Marker of Early Mesenchymal Progenitor Cells in Donor Bone Marrow Stroma

Vetoshkin¹,

Исаева²,

Butolina³

et al. 2020

Клиническая онкогематология

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Aim. To analyze the growth rate of mesenchymal stromal cell (MSC) culture depending on the aldehyde dehydrogenase-positive (ALDH+) cell count. Materials & Methods. The study involved bone marrow mesenchymal cell cultures of 10 donors (5 men and 5 women) with median age of 34.5 years (range 14-38 years). Nucleated cells were obtained by density gradient centrifugation. MSCs were cultivated according to the conventional protocol using platelet-rich donor plasma. Stromal cell identification and ALDH+ cell counting were performed by laser flow cytometry according to the criteria of the International Society for Cell Therapy. Results. The growth rate of MSC cultures and ALDH+ cell counts are maximum at primary and passage No. 1, becoming significantly lower by passage No. 3. The relationship between MSC culture growth rate and ALDH+ cell count was revealed. The older the donor, the lower MSC culture growth rate and ALDH+ cell count in bone marrow stroma. Conclusion. The data obtained indicate the relationship between bone marrow MSC culture growth rate, donor’s age, and ALDH+ cell count. ALDH-expressing cells proved to confer MSC population renewal. Based on the results acquired, we assume that the studied ALDH marker can serve as an objective criterion for placing mesenchymal cell elements into the category of early progenitor cells.

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Aldehyde dehydrogenase activity of Wharton jelly mesenchymal stromal cells: isolation and characterization

Cited by 6 publications

References 85 publications

Gene Expression Profiles of Human Mesenchymal Stromal Cells Derived from Wharton’s Jelly and Amniotic Membrane before and after Osteo-Induction Using NanoString Platform

Gene Expression Profiles of Human Mesenchymal Stromal Cells Derived from Wharton’s Jelly and Amniotic Membrane before and after Osteo-Induction Using NanoString Platform

Insight into Hypoxia Stemness Control

Aldehyde Dehydrogenase as a Marker of Early Mesenchymal Progenitor Cells in Donor Bone Marrow Stroma

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