Potential Involvement of BIRC5 in Maintaining Pluripotency and Cell Differentiation of Human Stem Cells

Abstract

The BIRC5 gene encodes a survivin protein belonging to class III of inhibitors of apoptosis, IAP. This protein serves a dual role. First, it regulates cell death, and second, it is an important regulator of mitosis progression, although its physiological regulatory function has not been fully understood. Many studies have shown and confirmed that survivin is practically absent in mature tissues in nature, while its overexpression has been reported in many cancerous tissues. There is little information about the significance of BIRC5 expression in normal adult human stem cells. This paper presents the study and analysis of survivin expression at the transcription level using qPCR method, in hematopoietic stem cells from peripheral blood mobilized with a granulocyte growth factor, adherent cells derived from the umbilical cord, and normal bone marrow stem cells. The expression of this gene was also examined in the blood of normal healthy individuals. The results of the analysis have shown that the more mature the cells are, the lower the expression of the BIRC5 gene is. The lowest expression has been found in peripheral blood cells, while the highest in normal bone marrow cells. The more the CD34+ and CD105 cells in the tested material are, the higher the BIRC5 expression is. Stem cells from cell culture show higher BIRC5 expression. The study confirms the involvement of BIRC5 from the IAP family in many physiological processes apart from apoptosis inhibition. The possible effect of BIRC5 on cell proliferation; involvement in cell cycle, cell differentiation, survival, and maintenance of stem cells; and the possible effect of IAP on the antineoplastic properties of mesenchymal stem cells have been demonstrated. Our research suggests that BIRC5 may be responsible for the condition of stem cell pluripotency and its high expression may also be responsible for the dedifferentiation of tumor cells.

Different regulation of PARP1, PARP2, PARP3 and TRPM2 genes expression in acute myeloid leukemia cells

“…(Life Technologies). Detailed procedures for the cells isolation, reverse transcription and real time PCR reactions have been described in our previous work, where we showed that the studied hematopoietic stem cells of peripheral blood and healthy bone marrow cells express the high level of BIRC5 gene expression, which confirms native nature of the tested hematopoetic stem cells [27].…”

Increased Expression of BIRC2, BIRC3, and BIRC5 from the IAP Family in Mesenchymal Stem Cells of the Umbilical Cord Wharton’s Jelly (WJSC) in Younger Women Giving Birth Naturally

“…Moreover, in the light of our previous research [34] in which we suggested that BIRC5 is involved in maintaining the state of pluripotency and stem cell differentiation, the higher BIRC5 expression demonstrated in the current study may indicate a more stem cell-like character of the isolated cells, their greater potential for differentiation, and thus better clinical utility of stem cells obtained from the umbilical cord from women after a natural childbirth.…”

“…Mesenchymal stem cells were isolated from the umbilical cord Wharton's jelly using the explant method [34]. To demonstrate the stem cell character of the isolated cells, cytometric analysis was performed and the presence of CD90 and CD105 surface antigens was demonstrated in the majority of the population studied [35].…”

“…The expression of the BIRC2, BIRC3, and BIRC5 genes was assessed using the qPCR method. For the study, synthesized cDNA (1 μL per sample) was used, as well as reagents from Applied Biosystems: the Gene Expression Master Mix buffer and TaqMan probes (for the BIRC2 gene: Hs_ 00357350_mL; for the BIRC3 gene: Hs_00154109_mL; for the BIRC5 gene: Hs_00153353_mL; and for the endogenous control GAPDH: Hs_99999905_mL [34].…”

Influence of the Type of Delivery, Use of Oxytocin, and Maternal Age on POU5F1 Gene Expression in Stem Cells Derived from Wharton’s Jelly within the Umbilical Cord

“…Monoclonal fluorescently labeled mouse anti-human CD90 PC5-labeled by Beckman Coulter and mouse anti-human CD105 PE-labeled by Beckman Coulter were used to assess the phenotype of the [14]. Detailed procedures for the isolation of reverse PCR transcription and real-time PCR reactions have been described in our work [15]. The statistical analysis was performed using the Statistica v. 13.0 software.…”