Influence of the Type of Delivery, Use of Oxytocin, and Maternal Age on <i>POU5F1</i> Gene Expression in Stem Cells Derived from Wharton’s Jelly within the Umbilical Cord

Gil-Kulik, Paulina; Chomik, Piotr; Krzyżanowski, Arkadiusz; Radzikowska-Büchner, Elżbieta; Maciejewski, Ryszard; Kwaśniewska, Anna; Rahnama-Hezavah, Mansur; Kocki, Janusz

doi:10.1155/2019/1027106

Cited by 8 publications

(8 citation statements)

References 55 publications

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“…It was shown that in the group of patients giving birth naturally, the expression of the BIRC2 gene is almost 3 times statistically significantly higher, the expression of the BIRC5 gene is more than 3.5 times higher, and the expression of the BIRC3 gene tends to have higher values in WJSC compared to patients giving birth by cesarean section. Moreover, in our previous studies, we have shown that the expression of the POU5F1 gene, which is responsible for cell stemness, is significantly higher in Wharton's jelly stem cells from women after natural delivery, in comparison with women giving birth by caesarean section [37].…”

Section: The Effect Of the Methods Of Delivery On The Expression Of Gementioning

confidence: 86%

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Increased Expression of BIRC2, BIRC3, and BIRC5 from the IAP Family in Mesenchymal Stem Cells of the Umbilical Cord Wharton’s Jelly (WJSC) in Younger Women Giving Birth Naturally

Gil-Kulik

Świstowska

Kondracka

et al. 2020

Oxidative Medicine and Cellular Longevity

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The knowledge of factors affecting the viability as well as proliferation and therapeutic potential of perinatal stem cells is of great importance for the decisions concerning their collection, multiplication, and storing. The aim of this work is to evaluate the expression of the BIRC2, BIRC3, and BIRC5 genes at the level of transcription in mesenchymal stem cells derived from the umbilical cord Wharton's jelly. The study examined the relationship between the expression level of the studied genes and selected biophysical parameters of umbilical blood: pH, pCO 2 , pO 2 , and cHCO 3 . Moreover, the relationship between the pregnant age, the type of delivery (natural delivery or cesarean section), and the level of expression of the BIRC2, BIRC3, and BIRC5 genes was assessed. The research was carried out on mesenchymal stem cells derived from the umbilical cord Wharton's jelly (WJSC) taken from 55 women immediately after delivery. Expression of the examined genes was assessed with the qPCR method using commercially available reagent kits. On the basis of the conducted research, it was demonstrated that WJSCs collected from younger women giving birth naturally, and in the acidic environment of the umbilical cord blood, are characterized by a higher expression of the BIRC2, BIRC3, and BIRC5 genes. It was shown that the expression of the BIRC2 and BIRC3 genes in Wharton's jelly mesenchymal stem cells declines with the mother's age. Our research suggests that stem cells collected from younger women giving birth naturally can be more resistant to apoptosis and show a more stem cell-like character, which can increase their therapeutic potential and clinical utility, but this conclusion needs to be approved in the next studies.

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Section: The Effect Of the Methods Of Delivery On The Expression Of Gementioning

confidence: 86%

“…During cell culture, the fibroblast-like shape of the cells and their ability to adhere to plastic walls were confirmed. The expression of SOX2 [35] and POU5F1 [37] genes was demonstrated in the examined cells; also, the expression of the SOX9 gene, characteristic of cell differentiation towards chondrocytes, was examined in cells (unpublished own study).…”

mentioning

confidence: 99%

Increased Expression of BIRC2, BIRC3, and BIRC5 from the IAP Family in Mesenchymal Stem Cells of the Umbilical Cord Wharton’s Jelly (WJSC) in Younger Women Giving Birth Naturally

Gil-Kulik

Świstowska

Kondracka

et al. 2020

Oxidative Medicine and Cellular Longevity

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“…No studies have been found with regard to the influence of the parturient’s age on the SOX2 expression level in WJSC. In the authors’ previous research, it was shown that the expression of the POU5F1 gene [ 58 ] and the expression of the BIRC2 , BIRC3 and BIRC5 genes [ 59 ] decrease with maternal age. It was noted that the expression of the SOX2 gene is statistically significantly higher in WJSC of babies born prematurely, and the level of the SOX2 expression correlated positively with the length of pregnancy.…”

Section: Discussionmentioning

confidence: 99%

“…In the author’s previous work, it was demonstrated that the expression of the second key factor responsible for maintaining the state of pluripotency, POU5F1 in stem cells of Wharton’s jelly cord, is dependent on the age of the gravida, the manner of delivery, the method of delivery and the use of oxytocin. MSCs derived from Wharton’s jelly (WJSC) taken from younger women and during their first childbirth as well as from patients who received oxytocin showed higher expression of POU5F1 [ 58 ]. In the authors’ previous studies, it was also observed that MSCs derived from Wharton’s jelly (WJSC) collected from younger women who were giving birth naturally and in an acidic umbilical cord blood environment are characterized by higher expression of inhibitor of apoptosis protein (IAP): BIRC2, BIRC3 and BIRC5 genes, making them more resistant to apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Impact of Pregnancy-Associated Factors on the Quality of Wharton’s Jelly-Derived Stem Cells Using SOX2 Gene Expression as a Marker

Gil-Kulik

Świstowska

Krzyżanowski

et al. 2022

IJMS

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SOX2 is a recognized pluripotent transcription factor involved in stem cell homeostasis, self-renewal and reprogramming. It belongs to, one of the SRY-related HMG-box (SOX) family of transcription factors, taking part in the regulation of embryonic development and determination of cell fate. Among other functions, SOX2 promotes proliferation, survival, invasion, metastasis, cancer stemness, and drug resistance. SOX2 interacts with other transcription factors in multiple signaling pathways to control growth and survival. The aim of the study was to determine the effect of a parturient’s age, umbilical cord blood pH and length of pregnancy on the quality of stem cells derived from Wharton’s jelly (WJSC) by looking at birth weight and using SOX2 gene expression as a marker. Using qPCR the authors, evaluated the expression of SOX2 in WJSC acquired from the umbilical cords of 30 women right after the delivery. The results showed a significant correlation between the birth weight and the expression of SOX2 in WJSC in relation to maternal age, umbilical cord blood pH, and the length of pregnancy. The authors observed that the younger the woman and the lower the umbilical cord blood pH, the earlier the delivery occurs, the lower the birth weight and the higher SOX2 gene expression in WJSC. In research studies and clinical applications of regenerative medicine utilizing mesenchymal stem cells derived from Wharton’s Jelly of the umbilical cord, assessment of maternal and embryonic factors influencing the quality of cells is critical.

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“…The biological activity of isolated MSCs depends on local, systemic and environmental factors. Recent research has shown that the ability of umbilical cord–derived stem cells to self-renew and survive depends on the age of the mother [ 30 ]. Therefore, the origin of the isolated MSCs may determine the success of using MSCs in research and therapy.…”

Section: General Properties Of Mscsmentioning

confidence: 99%

MSCs as Tumor-Specific Vectors for the Delivery of Anticancer Agents—A Potential Therapeutic Strategy in Cancer Diseases: Perspectives for Quinazoline Derivatives

Szewc

Radzikowska-Büchner

Wdowiak

et al. 2022

IJMS

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Mesenchymal stem cells (MSCs) are considered to be a powerful tool in the treatment of various diseases. Scientists are particularly interested in the possibility of using MSCs in cancer therapy. The research carried out so far has shown that MSCs possess both potential pro-oncogenic and anti-oncogenic properties. It has been confirmed that MSCs can regulate tumor cell growth through a paracrine mechanism, and molecules secreted by MSCs can promote or block a variety of signaling pathways. These findings may be crucial in the development of new MSC-based cell therapeutic strategies. The abilities of MSCs such as tumor tropism, deep migration and immune evasion have evoked considerable interest in their use as tumor-specific vectors for small-molecule anticancer agents. Studies have shown that MSCs can be successfully loaded with chemotherapeutic drugs such as gemcitabine and paclitaxel, and can release them at the site of primary and metastatic neoplasms. The inhibitory effect of MSCs loaded with anti-cancer agents on the proliferation of cancer cells has also been observed. However, not all known chemotherapeutic agents can be used in this approach, mainly due to their cytotoxicity towards MSCs and insufficient loading and release capacity. Quinazoline derivatives appear to be an attractive choice for this therapeutic solution due to their biological and pharmacological properties. There are several quinazolines that have been approved for clinical use as anticancer drugs by the US Food and Drug Administration (FDA). It gives hope that the synthesis of new quinazoline derivatives and the development of methods of their application may contribute to the establishment of highly effective therapies for oncological patients. However, a deeper understanding of interactions between MSCs and tumor cells, and the exploration of the possibilities of using quinazoline derivatives in MSC-based therapy is necessary to achieve this goal. The aim of this review is to discuss the prospects for using MSC-based cell therapy in cancer treatment and the potential use of quinazolines in this procedure.

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Influence of the Type of Delivery, Use of Oxytocin, and Maternal Age on POU5F1 Gene Expression in Stem Cells Derived from Wharton’s Jelly within the Umbilical Cord

Cited by 8 publications

References 55 publications

Increased Expression of BIRC2, BIRC3, and BIRC5 from the IAP Family in Mesenchymal Stem Cells of the Umbilical Cord Wharton’s Jelly (WJSC) in Younger Women Giving Birth Naturally

Increased Expression of BIRC2, BIRC3, and BIRC5 from the IAP Family in Mesenchymal Stem Cells of the Umbilical Cord Wharton’s Jelly (WJSC) in Younger Women Giving Birth Naturally

Evaluation of the Impact of Pregnancy-Associated Factors on the Quality of Wharton’s Jelly-Derived Stem Cells Using SOX2 Gene Expression as a Marker

MSCs as Tumor-Specific Vectors for the Delivery of Anticancer Agents—A Potential Therapeutic Strategy in Cancer Diseases: Perspectives for Quinazoline Derivatives

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