Phenotypic and genotypic characterization of Salmonella Typhimurium isolates from humans and foods in Brazil

Seribelli, Amanda Aparecida; Cruz, Marcelo Ferreira da; Vilela, Felipe Pinheiro; Frazão, Miliane Rodrigues; Paziani, Mário Henrique; Almeida, Fernanda Martins de; Medeiros, Marta Inês Cazentini; Rodrigues, Dália dos Prazeres; Kress, Márcia Regina von Zeska; Allard, Marc W.; Falcão, Juliana Pfrimer

doi:10.1371/journal.pone.0237886

Cited by 13 publications

(9 citation statements)

References 37 publications

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“…Of note, high isolation rates of S. typhimurium were also noticed. S. typhimurium remains one of the main serotype that can lead to sever human and animal diseases ( Amanda et al., 2020 ). Therefore, it is necessary to continuously monitor the local serovar variation of Salmonella and formulate a reasonable prevention and control strategy accordingly.…”

Section: Discussionmentioning

confidence: 99%

Characterization of integrons and antimicrobial resistance in Salmonella from broilers in Shandong, China

Zhao

Zhang

et al. 2020

Poultry Science

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Salmonella spp. are one of the most important foodborne bacterial pathogens in human beings and animals. This study aimed to analyze the prevalence and characterization of Salmonella from broilers in Shandong, China. A total of 67 Salmonella were recovered from 600 rectal swabs collected from 3 large-scale intensive broiler farms (67/600, 11.2%) between May and October 2018. Among Salmonella isolates, the most common serovars were S. enteritidis and S. typhimurium . The highest occurrence of resistance observed was for polymyxin (100%), followed by ampicillin (68.7%). The multidrug-resistant Salmonella isolation rate was observed to be 53.7%. Four β-lactamase genes were detected among the isolates, and all the isolates carried bla TEM (67/67, 100%), followed by bla OXA (19/67, 28.4%), bla CTX-M (17/67, 25.4%), and bla PSE (7/67, 10.4%). Four plasmid-mediated quinolone resistance gene were detected among the isolates; the prevalent resistance genes was aac(6′)-Ib-cr (18/67, 26.9%), followed by oqxB (9/67, 13.4%), qnrB (6/67, 9.0%), and qnrD (1/67, 1.5%). The prevalent rate of mcr -1 was 6.0% (4/67). Class 1 integrons were detected in 26.9% of these isolates and contained 7 groups of resistance gene cassettes. Multilocus sequence typing analysis revealed 7 sequence types, and ST11 was the most frequent sequence types. This study indicated that reduction of Salmonella and strict control on the use of antibiotics in more than 5,000 million broilers in Shandong are the vitally important measures to keep public health.

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Section: Discussionmentioning

confidence: 99%

Characterization of integrons and antimicrobial resistance in Salmonella from broilers in Shandong, China

Zhao

Zhang

et al. 2020

Poultry Science

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“…While it is thought that there is no discernible difference in the incidence or virulence of plasmids in isolated from either food and human-associated S. enterica ser. Typhimurium isolates, there is evidence that the virulence of pathogens can be enhanced through the selective processes encountered within the meat production chain [113]. Biofilm communities which can form in processing environments can facilitate the exchange of virulence-related genes such as fimbriae or toxin production through MGEs, and signals exchanged in these settings can induce the enhanced expression of virulence and survival factors [9,31,50].…”

Section: Consequences Of Bacterial Adaptive Pathwaysmentioning

confidence: 99%

“… Salmonella Type three secretion systems (T3SS), for example, are upregulated in cells within biofilms. These systems are associated with the Salmonella Pathogenicity Island (SPI)−1 virulence gene locus [50, 113]. Autoinducer-2 (AI-2) signalling can also enhance virulence gene expression, particularly in biofilms [88].…”

Section: Bacterial Responses To Stimulimentioning

confidence: 99%

“…Plasmid carriage of virulence determinants is important for their spread in close environments, leading to a shared core genome. S. enterica serovars Typhimurium, Enteritidis and Dublin have been found to carry hybrid virulence-resistance plasmids [102,105,113,120]. Virulence determinants carried by virulence and hybrid plasmids like pUO-SeVR1 and pSLT influence the severity of disease caused by these serovars (Table 3), and it is important to consider the spread of these MGEs to the human population from foods [92,105,120].…”

Section: Consequences Of Bacterial Adaptive Pathwaysmentioning

confidence: 99%

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Stress response modulation: the key to survival of pathogenic and spoilage bacteria during poultry processing

et al. 2022

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The control of bacterial contaminants on meat is a key area of interest in the food industry. Bacteria are exposed to a variety of stresses during broiler processing which challenge bacterial structures and metabolic pathways causing death or sublethal injury. To counter these stresses, bacteria possess robust response systems that can induce shifts in the transcriptome and proteome to enable survival. Effective adaptive responses, such as biofilm formation, shock protein production and metabolic flexibility, require rapid induction and implementation at a cellular and community level to facilitate bacterial survival in adverse conditions. This review aims to provide an overview of the scientific literature pertaining to the regulation of complex adaptive processes used by bacteria to survive the processing environment, with particular focus on species that impact the quality and safety of poultry products like Campylobacter spp., Salmonella enterica and Pseudomonas spp.

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“…It is found to be in the transmembrane of the bacteria. The gene ssaD encodes for the proteins related to the basal body, cytoplasmic rings and export apparatus and it is also involved in the ATPase complex, regulation and translocation of T3SS [81]. A study shows that there is an important defect in the intercellular survival with the mutant ssaD strains on comparing with the wild-type Salmonella [82].…”

Section: Features Of the Twenty Virulent Proteinsmentioning

confidence: 99%

Computational Identification of the Plausible Molecular Vaccine Candidates of Multidrug-Resistant Salmonella enterica

Mishra¹,

Tan²,

El-Aal³

et al. 2021

Salmonella Spp. - A Global Challenge

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Salmonella enterica serovars are responsible for the life-threatening, fatal, invasive diseases that are common in children and young adults. According to the most recent estimates, globally, there are approximately 11–20 million cases of morbidity and between 128,000 and 161,000 mortality per year. The high incidence rates of diseases like typhoid, caused by the serovars Typhi and Paratyphi, and gastroenteritis, caused by the non-typhoidal Salmonellae, have become worse, with the ever-increasing pathogenic strains being resistant to fluoroquinolones or almost even the third generation cephalosporins, such as ciprofloxacin and ceftriaxone. With vaccination still being one of the chosen methods of eradicating this disease, identification of candidate proteins, to be utilized for effective molecular vaccines, has probably remained a challenging issue. In our study here, we portray the usage of computational tools to analyze and predict potential vaccine candidate(s) for the multi-drug resistant serovars of S. enterica.

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Phenotypic and genotypic characterization of Salmonella Typhimurium isolates from humans and foods in Brazil

Cited by 13 publications

References 37 publications

Characterization of integrons and antimicrobial resistance in Salmonella from broilers in Shandong, China

Characterization of integrons and antimicrobial resistance in Salmonella from broilers in Shandong, China

Stress response modulation: the key to survival of pathogenic and spoilage bacteria during poultry processing

Computational Identification of the Plausible Molecular Vaccine Candidates of Multidrug-Resistant Salmonella enterica

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