Characterization of integrons and antimicrobial resistance in Salmonella from broilers in Shandong, China

Abstract

Salmonella spp. are one of the most important foodborne bacterial pathogens in human beings and animals. This study aimed to analyze the prevalence and characterization of Salmonella from broilers in Shandong, China. A total of 67 Salmonella were recovered from 600 rectal swabs collected from 3 large-scale intensive broiler farms (67/600, 11.2%) between May and October 2018. Among Salmonella isolates, the most common serovars were S. enteritidis and S. typhimurium . The highest occurrence of resistance observed was for polymyxin (100%), followed by ampicillin (68.7%). The multidrug-resistant Salmonella isolation rate was observed to be 53.7%. Four β-lactamase genes were detected among the isolates, and all the isolates carried bla TEM (67/67, 100%), followed by bla OXA (19/67, 28.4%), bla CTX-M (17/67, 25.4%), and bla PSE (7/67, 10.4%). Four plasmid-mediated quinolone resistance gene were detected among the isolates; the prevalent resistance genes was aac(6′)-Ib-cr (18/67, 26.9%), followed by oqxB (9/67, 13.4%), qnrB (6/67, 9.0%), and qnrD (1/67, 1.5%). The prevalent rate of mcr -1 was 6.0% (4/67). Class 1 integrons were detected in 26.9% of these isolates and contained 7 groups of resistance gene cassettes. Multilocus sequence typing analysis revealed 7 sequence types, and ST11 was the most frequent sequence types. This study indicated that reduction of Salmonella and strict control on the use of antibiotics in more than 5,000 million broilers in Shandong are the vitally important measures to keep public health.

Rapid Detection and Differentiating of the Predominant Salmonella Serovars in Chicken Farm by TaqMan Multiplex Real-Time PCR Assay

“…Gallinarum, S . Enteritidis, and S. Typhimurium were the prevalent pathogens of salmonellosis in chicken farms ( Medalla et al., 2016 ; Wang et al., 2020 ; Xu et al., 2020 ; Zhao et al., 2020 ; Yu et al., 2021 ). In addition, S .…”

“…Enteritidis), and Salmonella Typhimurium ( S . Typhimurium) ( Medalla et al., 2016 ; Wang et al., 2020 ; Xu et al., 2020 ; Zhao et al., 2020 ; Yu et al., 2021 ). These Salmonella serovars can lead to serious avian salmonellosis, which causes economic losses in the poultry industry.…”

Prevalence of <i>Salmonella</i> and Antimicrobial Resistance in Isolates from Food Animals — Six PLADs, China, 2019

“…High prevalence of resistance to ampicillin is due to this antibiotic has been widely used in animal husbandry in China ( 2 ). At present, clinics have reported high resistance of Salmonella to ampicillin (53%) ( 3 ) and high resistance in food animals (68.7%) in China ( 4 ). We should carry out regular disinfection and sterilization of the breeding environment and avoid eating meat, eggs, and other dairy products that have not been treated with high temperatures.…”

Salmonelose Aviária

“…No entanto, as diferenças entre as ganjas não foram associadas ao uso de antibióticos (Liljebjelke et al, 2017). Entretanto, Zhao et al (2020) analisando a prevalência de Salmonellas em criações intensivas de frangos de corte na China, relatam uma grande quantidade de isolados multirresistentes portadores de genes relacionados a resistência aos antibióticos beta-lactâmicos (blaTEM, blaOXA, blaCTX-M e blaPSE) e as quinolones (aac(6')-Ib-cr, oqxB, qnrB e qnrD), enfatizando que o controle rigoroso do uso de antibióticos nas granjas, bem como o controle da presença de Salmonella nas aves são medidas de vital importância para a saúde pública.…”